DNA vaccine containing the mycobacterial hsp65 gene prevented insulitis in MLD-STZ diabetes

Abstract

Background: Our group previously demonstrated that a DNA plasmid encoding the mycobacterial 65-kDa heat shock protein (DNA-HSP65) displayed prophylactic and therapeutic effect in a mice model for tuberculosis. This protection was attributed to induction of a strong cellular immunity against HSP65. As specific immunity to HSP60 family has been detected in arthritis, multiple sclerosis and diabetes, the vaccination procedure with DNA-HSP65 could induce a cross-reactive immune response that could trigger or worsen these autoimmune diseases.

Selecting probiotics with the abilities of enhancing GLP-1 to mitigate the progression of type 1 diabetes in vitro and in vivo

“…Previous studies have revealed that the preponderance of the Th2-type cytokines IL-4 and IL-10 prevented the destruction of β cells (Müller, Schott-Ohly, Dohle, & Gleichmann, 2002;Santos et al, 2009). Increases in the production of IL-10 are generally considered an antiinflammatory cytokine that acts mostly on antigen-presenting cells by inhibiting antigen presentation and the production of inflammatory cytokines.…”

Filarial Abundant Larval Transcript Protein ALT-2: An Immunomodulatory Therapeutic Agent for Type 1 Diabetes

“…within 15 min. of preparation) for five consecutive days [19]. The mice were monitored for fasting glucose levels in blood samples (obtained from the tail vein of mice after 6 h of fasting) once in a week starting from 7 days after the last dose of STZ.…”

Prevention of experimental diabetes by Uncaria tomentosa extract: Th2 polarization, regulatory T cell preservation or both?

“…The result was expressed as the percentage of islet area showing mononuclear cell infiltration, according to the formula: mononuclear infiltrate area (m 2 ) × 100/islet area (m 2 ). Finally, the results were categorized into the following score: 0 = intact islet; 1 = peri-insulitis (≤10% of the islet infiltrated); 2 = moderate insulitis (≤50% of the islet infiltrated); 3 = severe insulitis (≥50% of the islet infiltrated); and 4 = destructive insulitis (Santos et al, 2009). At least 20 islets per pancreas were analyzed in non-consecutive sections.…”

“…Peripheral changes such as downregulation of Th2-derived cytokines and inhibition of Treg cells were also demonstrated (Zdravkovic et al, 2009). One of the advantages of this model is the fact that hyperglycemia and insulitis are observed in a relatively short period of time (2-3 weeks after the last STZ injection) in a high percentage of animals (Paik et al, 1980;Santos et al, 2009).…”