Selecting probiotics with the abilities of enhancing GLP-1 to mitigate the progression of type 1 diabetes in vitro and in vivo

Wei, Shih-Hung; Chen, Yen‐Po; Chen, Ming‐Ju

doi:10.1016/j.jff.2015.08.016

Cited by 50 publications

(33 citation statements)

References 43 publications

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“…In our previous study, we found that oral lactic acid bacteria reduced blood glucose and prevented heart injury in rats with streptozotocin-induced diabetes mellitus (DM) 31 . Moreover, lactic acid bacteria supplementation inhibited the pro-inflammatory and inflammatory cytokines secreted in type 1 DM animal model 32 and down-regulated insulin levels in a type 2 DM animal model 33 , 34 . However, the application of probiotic for the prevention of cardiomyocyte apoptosis induced by high calorie-induced obesity and CVD have not been extensively explored 33 .…”

Section: Introductionmentioning

confidence: 96%

Heat-killed Lactobacillus Reuteri GMNL-263 Prevents Epididymal Fat Accumulation and Cardiac Injury in High-Calorie Diet-Fed Rats

Liao¹,

Kuo²,

Hsieh³

et al. 2016

Int. J. Med. Sci.

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High-calorie diet-induced obesity leads to cardiomyocyte dysfunction and apoptosis. Impaired regulation of epididymal fat content in obese patients has been known to increase the risk of cardiac injury. In our study, a lactic acid bacteria, Lactobacillus reuteri GMNL-263, was evaluated for its potential to reduce body weight and body fat ratio and to prevent heart injury in rats with high-fat diet-induced obesity. Lactic acid bacteria supplementation restored the cardiac function and decreased the physiological changes in the heart of the obese rats. In addition, the Fas/Fas-associated protein pathway-induced caspase 3/e Poly polymerase mediated apoptosis in the cardiomyocytes of the obese rats was reversed in the Lr263-treated rats. These results reveal that fed with Lr-263 reduces body fat ratio, inhibits caspase 3-mediated apoptosis and restores cardiac function in obese rats through recovery of ejection fraction and fractional shortening. Our results indicated that the administration of Lr263 lactic acid bacteria can significantly down-regulate body fat and prevent cardiomyocyte injury in obese rats.

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Section: Introductionmentioning

confidence: 96%

Heat-killed Lactobacillus Reuteri GMNL-263 Prevents Epididymal Fat Accumulation and Cardiac Injury in High-Calorie Diet-Fed Rats

Liao¹,

Kuo²,

Hsieh³

et al. 2016

Int. J. Med. Sci.

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“…The STC-1 cells used in this study were purchased from Shanghai Enzyme Research Biotechnology Co. Ltd (Shanghai, China). They were cultured in Dulbecco's modified Eagle's medium high-sugar medium containing 10% foetal bovine serum (Hyclone, UT) at 37°C with an atmosphere of 5% CO 2 and 95% air (Wei et al 2015). The HT-29 cells were obtained from Laboratory of Probiotics and Fermented Foods, Harbin Institute of Technology (Harbin, China), and were cultured in Roswell Park Memorial Institute-1640 medium containing 10% foetal bovine serum (Hyclone) under the aforementioned conditions (Wang et al 2014).…”

Section: Cell Culture Conditionsmentioning

confidence: 99%

“…The bacteria were then harvested by centrifugation at 5000 g for 5 min and washed with sterile PBS buffer three times. The bacteria cells (1 9 10 7 CFU) treated with the simulated gastric/intestinal juice were tested for the ability to promote PYY secretion by STC-1 cells according to the previous method (Wei et al 2015). The concentration of PYY were determined using an enzyme-linked immunosorbent assay (Mouse PYY ELISA KIT; Longton, Shanghai, China).…”

Section: Selecting Strains With the Abilities To Promote Pyy Secretionmentioning

confidence: 99%

Profiles of gut microbiota in children with obesity from Harbin, China and screening of strains with anti‐obesity ability in vitro and in vivo

Liang

Guo²,

Liu

et al. 2020

J Appl Microbiol

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Aims: The purpose of this study was to investigate the characteristics of gut microbiota of children with obesity in Harbin, China and to screen antiobesity strains in vitro and in vivo. Methods and Results: The gut microbiota of children with obesity and normal-weight children were investigated by high-throughput sequencing, and based on the different composition in gut microbiota, the strains with potential anti-obesity properties were screened in vitro and in vivo. Compared with normal-weight children, the Firmicutes to Bacteroidetes ratio in children with obesity decreased. Moreover the relative abundances of Lactobacillus and Bifidobacterium in children with obesity were decreased, while the relative abundance of Akkermansia increased. After a series of screening in vitro and in vivo, nine strains were found inhibiting the body weight gain of HFD-fed mice, of which two strains showed significant effects (P < 0Á05). Conclusions: There were significant changes in gut microbiota of children with obesity from Harbin, China. The obtained strains showed obvious antiobesity effects, and the screening methods used in this study were effective. Significance and Impact of the Study: This study enriched the research results on the characteristics of gut microbiota of children with obesity in different regions of the world. Moreover we established a new and effective method for screening anti-obesity strains, and obtained effective strains.

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“…19 The influence of human lactobacilli engineered to secrete GLP-1 on hyperglycemia has been investigated by Duanet al 14 They showed that these lactobacilli reprogram intestinal cells into glucose-responsive insulin-secreting cells and that they therefore had the ability to ameliorate hyperglycemia and diabetes.…”

Section: Also Administration Of Lactobacillus Kefiranofaciens M Andmentioning

confidence: 99%

Potential mechanisms linking probiotics to diabetes: a narrative review of the literature

et al. 2017

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CONTEXT AND OBJECTIVE: Some studies have suggested a wide range of possible mechanisms through which probiotics may play a role in diabetes prevention and treatment. However, the underlying mechanisms are not fully understood. We conducted this study to review the potential mechanisms suggested for the effect of probiotics in diabetes. DESIGN AND SETTING: Narrative review conducted at the Food Security Research Center of Isfahan. METHODS: A search in the electronic databases MEDLINE (PubMed), Cochrane Library, Web of Science and Google scholar was performed up to October 2016. RESULTS:The initial search yielded 1214 reports. After removing duplicates, 704 titles and abstracts were screened. Finally, out of 83 full-text articles that were reviewed for eligibility, 30 articles were included in the final analysis. The anti-diabetic mechanisms for probiotics reported encompass intraluminal and direct effects on the intestinal mucosa and microbiota (n = 13), anti-inflammatory and immunomodulatory effects (n = 10), antioxidative effects (n = 5), effects on endoplasmic reticulum (ER) stress and expression of genes involved in glucose homeostasis and insulin resistance (n = 6), with some studies pointing to more than one mechanism. CONCLUSION: The results may throw some light on the capacity of probiotics as a novel approach towards controlling diabetes. However, further human studies are warranted to elucidate and confirm the potential role of probiotics in diabetes prevention and treatment. Also, it needs to be ascertained whether the effectiveness of probiotics in diabetes prevention and treatment is dependent on the strain of the microorganisms. Os mecanismos antidiabéticos relatados dos probióticos abrangem efeitos intraluminais e diretos na mucosa e microbiota intestinal (n = 13), efeitos anti-inflamatórios e imunomoduladores (n = 10), efeitos antioxidativos (n = 5), efeitos sobre o estresse de retículo endoplasmático (RE) e expressão de genes envolvidos na homeostase da glicose e resistência à insulina (n = 6), com alguns estudos apontando para mais de um mecanismo. CONCLUSÃO: Os resultados podem lançar alguma luz sobre os probióticos como uma nova abordagem no controle do diabetes, no entanto, mais estudos em humanos são justificados para elucidar e confirmar o papel potencial dos probióticos na prevenção e tratamento do diabetes. Além disso, deverá ser determinado se a eficácia dos probióticos na prevenção e tratamento do diabetes é dependente da cepa dos microrganismos. RESUMO IPhD. Doctoral

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Selecting probiotics with the abilities of enhancing GLP-1 to mitigate the progression of type 1 diabetes in vitro and in vivo

Cited by 50 publications

References 43 publications

Heat-killed Lactobacillus Reuteri GMNL-263 Prevents Epididymal Fat Accumulation and Cardiac Injury in High-Calorie Diet-Fed Rats

Heat-killed Lactobacillus Reuteri GMNL-263 Prevents Epididymal Fat Accumulation and Cardiac Injury in High-Calorie Diet-Fed Rats

Profiles of gut microbiota in children with obesity from Harbin, China and screening of strains with anti‐obesity ability in vitro and in vivo

Potential mechanisms linking probiotics to diabetes: a narrative review of the literature

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