DNA-induced synthesis of biomimetic enzyme for sensitive detection of superoxide anions released from live cell

Abstract

In this work, we successfully fabricate a rapid, sensitive sensor for the detection of superoxide anions O2˙− based on graphene/DNA/Mn3(PO4)2 biomimetic enzyme.

Recent advances of graphene–biomacromolecule nanocomposites in medical applications

“…155 Xing et al fabricated a biomimetic enzymatic sensor based on graphene/ DNA/Mn 3 (PO 4 ) 2 nanosheets to detect superoxide anion. 55 The obtained nanocomposites exhibited significant biomimetic enzymatic activity, and can detect superoxide anions quickly and sensitively. This method has broad application prospects in biomedical research and clinical testing.…”

“…In another work, Ding and co-workers prepared a graphene/DNA/Mn 3 (PO 4 ) 2 composite with good electrochemical activity. 55 Graphene as a carrier improved the catalytic performance of Mn 3 (PO 4 ) 2 nanoparticles. DNA was adsorbed on graphene through p-p stacking without destroying the complete structure of the carbon material.…”

“…4 (a) Schematic illustration of the preparation procedure for the graphene/DNA/Mn 3 (PO 4 ) 2 . 55 Copyright 2018, Royal Society of Chemistry. (b) The schematic of the laser induced graphene biosensor for miRNA detection.…”

Detection Technologies for Reactive Oxygen Species: Fluorescence and Electrochemical Methods and Their Applications

“…Electrochemical techniques employ the same principle as the fluorescence method as they combine electrochemical reactions with specific sensing elements to selectively detect . Several publications between 2016 to 2020 showed the detection of in cancerous cells [ 89 , 90 ], tumorous cells [ 91 , 92 ], macrophages [ 93 , 94 ], skeletal muscle cells, human glioblastoma cells, and human keratinocyte cells [ 47 ]. However, there are still several limitations that need to be considered when implementing electrochemical techniques for detection, especially in tissues.…”