DNA Fingnerprinting of Eight Cyprinid Fish Species of Iraqi Inland Waters Using RAPD-PCR Technique

Abstract

One hundred and twenty specimens of eight cyprinid fish species included Barbus xanthopterus, B. kersin, B. barbulus, B. grypus, B. sharpeyi, B. luteus, Aspius vorax and Cyprinus carpio were collected from Shatt Al-Arab River in governorate of Basrah Southern of Iraq. The DNA fingerprints were identified using RAPD-PCR technique with seven decamere primers. A total of 223 anonymous scorable bands obtained by RAPD-PCR technique across eight cyprinid species genomes. The average of bands per primer was 32 bands, the size of bands estimated using the linear equation of standard curve plot of standard 100 bp ladder. The RAPD markers were used to obtain the similarity indices that were in high value among Barbus species since it ranged from 84.4% between B. xanthopterus and B. kersin to 52% between B. barbulus and B. sharpeyi. While the B. grypus appeared in high similarity indices value (86.9%) with luciscin A. vorax. While C. carpio exhibit high diversity indices value with the Barbus spp.. Therefore the phylogenetic relationships among the eight studied cyprinid species were clearly resolved in dendrograms, they clustered into three branches: the first one included B. xanthopterus, B. kersin, B, barbulus, while the second included B. sharpeyi and B. luteus and the third branch contained B. grypus and A. vorax. Nevertheless, when A. vorax data discarded, the B. grypus return to cluster with B. sharpeyi and B. luteus. Whereas C. carpio functioned as outgroup in both cases. The conclusion of the present study was: molecular patterns showed by RAPD bands represent specific DNA fingerprints and apparently are suitable tool to distinguish among cyprinids. The results clearly revealed that the eight cyprinid taxa are distinctive species. The studied Barbus species are divided into two groups, the first one included B. xanthopterus, B. kersin and B. barbulus which have European Luciobarbus affinities and the second contained B. grypus, B. sharpeyi and B. luteus which they diverged of Luciobarbus but still related to Barbus. So, the Barbus in Iraq is polyphyletic group.

Dna Fingerints of Tilapia Species in Shatt Al-Areb River Using Rapd Markers

“…These advantages make it more preferable than other techniques. The study agrees with local study accomplished to create DNA fingerprints of some cyprinid fish species in Iraq (15). The RAPD-PCR amplification with a single decamer primer to produce DNA fingerprint was affecting essentially with primers, DNA template and reaction conditions.…”

“…Furthermore, RAPD markers used to differentiate among Spanish Barbus species (8), some Iberian cyprinid species (9, 10), European sea bass populations (7), striped red mullet populations (20). Genetic similarity and diversity of cultured catfish (Silurus asotus) populations were analyzed (31) and Etroplus maculatus populations (18).Locally the RAPD markers were efficient to differentiate among Iraqi cyprinid fish species (15). Furthermore, it could separate the genus Barbus species from another related genus (13).…”

Detection of Genomic Instability in Renal Cancer by Random Amplified Polymorphic DNA Analysis from Urine Sample as a Non-invasive Method: Potential Use in Diagnosis

“…The randomly amplified polymorphic DNA (RAPD) protocol was reliable, simple to set up, fast and large areas of genomic DNA screened. The RAPD-PCR amplification with a single decamer primer is affected essentially with Primer, DNA template and reaction conditions [9]. So that these advantages in comparison to other DNA fingerprinting method, such as restriction fragment length polymorphism (RFLP) that need larger quantity of pure DNA, target gene or few loci of the gene and some information's about DNA restriction sites and restriction enzymes.…”

DNA Fingerprints of Two Tilapia Fish Species of Euphrates River at Governorate ofAl-Muthanna Using RAPD Markers

“…On the same side, Jini [23] used RAPD method to discriminate among Etroplus maculatus populations . Locally, RAPD genetic markers utilized to differentiate among eight cyprinid fish species of Iraqi inland waters [24]. While the same technique followed to discriminate among six Barbus species [25].…”