Detection of Genomic Instability in Renal Cancer by Random Amplified Polymorphic DNA Analysis from Urine Sample as a Non-invasive Method: Potential Use in Diagnosis

El‐Far, Mohamed

doi:10.15226/csroa.2013.00102

Cited by 1 publication

(1 citation statement)

References 16 publications

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“…The upper portion of the aqueous layer was shifted into a sterilized fresh microcentrifuge tube. 1 M sodium chloride solution (NaCl) was added and mixed well, then six-tenth volume of chilled isopropanol [8].…”

Section: Phenol-chloroform Dna Extractionmentioning

confidence: 99%

Detection of Gene Mutation Associated with Bladder Cancer through DNA Polymorphism

Bera,

Dasgupta

2024

Int. J. Res. Publ. Rev.

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The periodic statistic defines encyclopaedically, urothelial bladder melanoma ranks the fifth most common cancer in men and the fifteenth in women, and the prevalence rate increases in people aged than 60 (1). Oncologists and pathologists in Egypt have suggested that Egypt has one of the loftiest prevalence rates of bladder cancer among men in the world, estimated prevalence rate of37.7 cases per 100,000 males (2). The histopathological profile of bladder cancer has changed significantly over the once 26 times. Historically, scaled cell melanoma but now a days, transitional cell melanoma has come the most frequent type and this shows the change in the epidemiology of the complaint (3). Individual variation in the inheritable constitution of humans may affect the host responses to constant assaults from exogenous and endogenous carcinogens, which will ultimately impact cancer threat, complaint prognostic and clinical outgrowth. Then we're agitating about a number of associations and limitations of current inheritable vulnerability exploration and suggest unborn directions in molecular epidemiology study. DNA polymorphism, a condition in which one of two different but normal nucleotide sequences can live at a particular point in DNA, the appearance of heritable mutation in a population at high frequency. Since heritable mutations eventually lead to elaboration, hence study of DNA polymorphism is important from the evolutionary perspective for the progression of bladder cancer. Different alleles of a gene produce different phenotypes which can be detected by making crosses between parents with different alleles of two or further genes. Also, by determining recombinants in the get, a inheritable chart can be derived. DNA polymorphisms constitute molecularly defined differences between individual mortal beings; hence the dominant homozygous, heterozygous and sheepish homozygous alleles can be helpful for the identification of heritable bladder cancer normal, carrier and diseased independently. The present study aimed to find out the association of polymorphic variations in the glutathione S transferase gene (GSTP1) with the bladder cancer in an Eastern Indian Population. From worldwide and civil studies, it was hypothecated that the GSTP1 gene polymorphism is a common event in people suffering from bladder cancer. It was hypothecated that the PCR-RFLP fashion is a good and robust fashion for discovery of this polymorphism. Consequently, the present study was designed and executed.

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Section: Phenol-chloroform Dna Extractionmentioning

confidence: 99%

Detection of Gene Mutation Associated with Bladder Cancer through DNA Polymorphism

Bera,

Dasgupta

2024

Int. J. Res. Publ. Rev.

View full text Add to dashboard Cite

show abstract

Detection of Genomic Instability in Renal Cancer by Random Amplified Polymorphic DNA Analysis from Urine Sample as a Non-invasive Method: Potential Use in Diagnosis

Abstract: novel genomic markers and proving the role of RAPD-PCR in diagnosis of tumor development generally.

Cited by 1 publication

References 16 publications

Detection of Gene Mutation Associated with Bladder Cancer through DNA Polymorphism

Detection of Gene Mutation Associated with Bladder Cancer through DNA Polymorphism

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