DNA fingerprinting of renal cell carcinoma with special reference to tumor heterogeneity

Abstract

Genomic alterations in renal cell carcinoma were investigated by DNA fingerprinting using the simple repetitive oligonucleotide probe (CAC)s. Nine of ten tumors showed somatic changes in the fingerprint pattern compared with constitutional DNA. The most consistent changes were deletions and/or decrease in intensity of a band. When using two or three samples from different parts within the tumor, up to three different cell clones could be detected. These results indicate that DNA fingerprinting analysis can be a useful technique for the study of genomic alterations and tumor heterogeneity in renal cell carcinoma.

Heterogeneity in renal cell carcinoma and its impact on prognosis - a flow cytometric study

“…Discussion frequency of metachronous metastases in patients with aneuploid tumours (Table IV) (Mehle et al. 1993;Nordenson et al.…”

“…However, owing to dilution a small aneuploid clone could be undetected using the mixture approach. The evaluation of molecular genetic characteristics might also be improved by separate analysis of multiple samples as illustrated by DNA fingerprint patterns, telomere length fragment determinations (Mehle et al, 1993(Mehle et al, , 1994 and analysis of polymorphic microsatellite markers (unpublished data).…”

“…In renal cell carcinoma we have previously demonstrated heterogeneity regarding DNA ploidy, karyotype, DNA fingerprint pattern and cell kinetic properties (Larsson et al, 1993;Ljungberg et al, 1985Ljungberg et al, , 1994Mehle et al, 1993;Nordenson et al, 1988). The most powerful prognostic parameter in renal cell carcinoma is tumour stage but DNA ploidy and cell kinetic parameters have also been found to be of predictive value (Baretton et al, 1991;Larsson et al, 1993;Ljungberg et al, 1994;Skinner et al, 1971).…”

Nuclear DNA content-derived parameters correlated with heterogeneous expression of p53 andbcl-2 proteins in clear cell renal carcinomas

“…Moreover, DNA flow cytometry is an objective and quantitative technique that measures the degree of quantitative abnormalities of DNA content (i.e., ploidy) and the percentage of cells in the S‐phase of the cell cycle 3, 4. In human RCC, intratumor heterogeneity regarding the DNA content has been demonstrated by several authors,5–8 and ploidy and S‐phase fraction (SPF) have been found to be prognostic factors 7, 9, 10…”