DNA analysis in forensics, disease and animal/plant identification

In some legal proceedings, the species identification of animal on the basis of fragments of biological material is extremely difficult. This applies both to closely-related and to distant species characterized by similar morphological features. In such circumstances, methods of molecular biology are used, whose evidential value is definitely not in doubt. Histopathological scraps may also have to be used for identifying tests. The aim of the present study was to verify the possibility of using DNA analysis in determining the species of animals on the basis of biological material contained in archival histopathological samples. The examined material consisted of twenty-eight histopathological preparations stained with hematoxylin and eosin. The samples had been prepared from the liver, kidney, spleen, and skeletal muscles. Their age varied from one to seventeen years. Specimens (from twelve species) were identified by inputting sequences in the Barcode of Life Database species identification tool on the basis of the similarity percentage figure from the BOLD report. It was found that genetic tests can effectively identify animal species through the analysis of biological material from histopathological samples.

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“…Then, molecular biology methods should be used. Their evidential value is not in doubt (1,6,7,21,23,24).…”

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confidence: 92%

Use of DNA analysis of histopathological specimens in species identification for purposes of forensic veterinary medicine

Malewski

Sołtyszewski

Karaźniewicz

et al. 2019

Medycyna Weterynaryjna

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“…Short tandem repeats (STRs) are markers widely used for genetic linkage mapping, medical testing and forensic applications (1,5,7). The majority of these markers are analyzed as DNA fragments in the size range of 100-350 bp with repeating units of 2, 3, 4 or 5 bases.…”

Section: Introductionmentioning

confidence: 99%

Agarose-Based System for Separation of Short Tandem Repeat Loci

White¹,

Kusukawa²

1997

BioTechniques

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“…They are the most useful type of sequence variation for use as genetic markers because they are highly polymorphic,12,13 abundant in the genome (1 every 10–20 kb),10 and relatively easy to isolate using the polymerase chain reaction (PCR) 9,14,15. Additionally, they are directly linked to a class of primarily neurodegenerative genetic diseases (i.e., Huntington's disease, myotonic dystrophy)16 and are used for human identification 17. They vary in complexity from simple repeats to complex repeats with numerous base insertions 18.…”

mentioning

confidence: 99%

Determination of a correction to improve mass measurement accuracy of isotopically unresolved polymerase chain reaction amplicons by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry

Null

Muddiman

2003

Rapid Comm Mass Spectrometry

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The experimental determination of average mass by mass spectrometry is limited for large molecules due to the negative bias introduced by the natural distribution of isotopic abundances. This results in the measurement of the top-of-centroid (ToC) as opposed to the true centroid. We have developed a practical correction factor that is applied to the ToC measurement to largely remove the systematic bias introduced by nature. The correction factor is calculated easily using the average molecular mass (<100 kDa) of the analyte molecule and the full-width half maximum resolving power (<3,500) of the measurement. In addition, an approach to calculating resolving power is described that accurately predicts resolving power achievable for Fourier transform ion cyclotron resonance (FT-ICR) mass analysis of large molecules. A combination of internal calibration with a dual-electrospray source and application of the correction factor to average mass measurements improved the mass error from 192.5 to -35.0 ppm for a 44 kDa PCR amplicon.

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DNA analysis in forensics, disease and animal/plant identification

Cited by 26 publications

References 37 publications

Use of DNA analysis of histopathological specimens in species identification for purposes of forensic veterinary medicine

Use of DNA analysis of histopathological specimens in species identification for purposes of forensic veterinary medicine

Agarose-Based System for Separation of Short Tandem Repeat Loci

Determination of a correction to improve mass measurement accuracy of isotopically unresolved polymerase chain reaction amplicons by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry

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