DNA Fingerprinting, DNA Barcoding, and Next Generation Sequencing Technology in Plants

Sucher, Nikolaus J.; Hennell, James R.; Carles, Maria

doi:10.1007/978-1-61779-609-8_2

Cited by 38 publications

(21 citation statements)

References 51 publications

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“…In addition, DNA sequences can be deposited into public databases for genetic information like GenBank® and are, therefore, available for other scientists [146].…”

Section: Dna-based Methodsmentioning

confidence: 99%

The relevance of pharmacognosy in pharmacological research on herbal medicinal products

Pferschy‐Wenzig

Bauer

2015

Epilepsy & Behavior

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“…In addition, DNA sequences can be deposited into public databases for genetic information like GenBank® and are, therefore, available for other scientists [146].…”

Section: Dna-based Methodsmentioning

confidence: 99%

The relevance of pharmacognosy in pharmacological research on herbal medicinal products

Pferschy‐Wenzig

Bauer

2015

Epilepsy & Behavior

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“…The primary application of DNA barcodes will continue to be the identification of unknown samples. The adoption of NGS technologies for DNA barcoding will lead to tremendous growth in available sequence data, which will become a powerful molecular tool for species discovery, evolution and the conservation of biodiversity (Sucher et al, 2012).…”

Section: Next-generation Sequencing and Dna Barcodingmentioning

confidence: 99%

DNA barcoding: an efficient tool to overcome authentication challenges in the herbal market

Mishra

Kumar

Nagireddy

et al. 2015

Plant Biotechnology Journal

270

181

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biological reference material herbal barcode library. SummaryThe past couple of decades have witnessed global resurgence of herbal-based health care. As a result, the trade of raw drugs has surged globally. Accurate and fast scientific identification of the plant(s) is the key to success for the herbal drug industry. The conventional approach is to engage an expert taxonomist, who uses a mix of traditional and modern techniques for precise plant identification. However, for bulk identification at industrial scale, the process is protracted and time-consuming. DNA barcoding, on the other hand, offers an alternative and feasible taxonomic tool box for rapid and robust species identification. For the success of DNA barcode, the barcode loci must have sufficient information to differentiate unambiguously between closely related plant species and discover new cryptic species. For herbal plant identification, matK, rbcL, trnH-psbA, ITS, trnL-F, 5S-rRNA and 18S-rRNA have been used as successful DNA barcodes. Emerging advances in DNA barcoding coupled with next-generation sequencing and high-resolution melting curve analysis have paved the way for successful species-level resolution recovered from finished herbal products. Further, development of multilocus strategy and its application has provided new vistas to the DNA barcode-based plant identification for herbal drug industry. For successful and acceptable identification of herbal ingredients and a holistic quality control of the drug, DNA barcoding needs to work harmoniously with other components of the systems biology approach. We suggest that for effectively resolving authentication challenges associated with the herbal market, DNA barcoding must be used in conjunction with metabolomics along with need-based transcriptomics and proteomics.

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“…Despite the development of the next-generation sequencing, the DNA fingerprinting is still the preferred way of plant genetic resources (PGR) genetic characterization [10–13]. Molecular biology provides various methods based on the DNA polymorphism which are not affected by the environment; they require only a small amounts of biological material at any growth stage that can be easily transported from the collection site to a laboratory and stored for a long time [14].…”

Section: Introductionmentioning

confidence: 99%

Promoting the Use of Common Oat Genetic Resources through Diversity Analysis and Core Collection Construction

et al. 2016

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The assessment of diversity and population structure and construction of a core collection is beneficial for the efficient use and management of germplasm. A unique collection of common oat landraces, cultivated in the temperate climate of central Europe until the end of the twentieth century, is preserved in the Polish gene bank. It consists of 91 accessions that have never been used in breeding programs. In order to optimise the use of this genetic resource, we aimed to: (1) determine genetic and agro-morphological diversity, (2) identify internal genetic variation of the tested accessions, (3) form a core collection and (4) recognise the accessions useful for breeding programs or re-release for cultivation. The collection was screened using ISSR markers (1520 loci) and eight agro-morphological traits. Uniquely, we performed molecular studies based on 24 individuals of every accession instead of bulk samples. Therefore, assessment of the degree of diversity within each population and the identification of overlapping gene pools were possible. The observed internal diversity (Nei unbiased coefficient) was in the range of 0.17–0.31. Based on combined genetic and agro-morphological data, we established the core collection composed of 21 landraces. Due to valuable compositions of important traits, some accessions were also identified as useful for breeding programs. The population structure and principal coordinate analysis revealed two major clusters. Based on the previous results, the accessions classified within the smaller one were identified as obsolete varieties instead of landraces. Our results show that the oat landraces are, in general, resistant to local races of diseases, well adapted to local conditions and, in some cases, yielding at the level of modern varieties. Therefore, in situ conservation of the landraces in the near future may be satisfactory for both farmers and researchers in terms of the genetic resources preservation.

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DNA Fingerprinting, DNA Barcoding, and Next Generation Sequencing Technology in Plants

Cited by 38 publications

References 51 publications

The relevance of pharmacognosy in pharmacological research on herbal medicinal products

The relevance of pharmacognosy in pharmacological research on herbal medicinal products

DNA barcoding: an efficient tool to overcome authentication challenges in the herbal market

Promoting the Use of Common Oat Genetic Resources through Diversity Analysis and Core Collection Construction

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