DNA-directed capture of primary cells from a complex mixture and controlled orthogonal release monitored by SPR imaging

Bombera, Radoslaw; Leroy, Loïc; Livache, Thierry; Roupioz, Yoann

doi:10.1016/j.bios.2011.11.034

Cited by 27 publications

(30 citation statements)

References 40 publications

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“…However, SPR allows you to identify cell type in a label-free manner, whereas FACS requires labeling surface proteins prior to analysis. In addition, the SPR-based construct by Bombera et al discussed above offers a promising potential for developing a new cell sorting device at the individual cell level and a simple way to monitor both molecular and cellular interactions simultaneously using the same device [39].…”

Section: Application Of Spr In the Analysis Of Cell Receptor-ligand Imentioning

confidence: 98%

“…Conventional SPR, SPRi and long-range SPR (LRSPR) have each been used for assessing these cellular responses [15,17,[42][43][44]. Reproduced with permission from [39].…”

Section: Application Of Spr In the Analysis Of Cellular Behavior Undementioning

confidence: 98%

“…The purpose was to study the capture of specific immune cells and their controlled release through the injection of specific cleavage enzymes to the on-chip construction [36]. The controlled release was further exploited for cell sorting applications [39]; in which, the construction of the DNA-protein complex was monitored by the SPRi and then applied to capture live primary cells and ultimately allow their release ( Figure 3). In comparison to traditional methods like fluorescent-assisted cell sorting (FACS) [40] that allows you to separate and identify different types of cells, SPR does not offer the high-throughput FACS provide.…”

Section: Application Of Spr In the Analysis Of Cell Receptor-ligand Imentioning

confidence: 99%

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Surface Plasmon Resonance: a Label-Free Tool for Cellular Analysis

Zeidan

Kepley

Sayes

et al. 2015

Nanomedicine (Lond.)

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Surface plasmon resonance (SPR) is a popular technique that allows for sensitive, specific, label-free and real-time assessment of biomolecular interactions. SPR is a nondestructive, modular and flexible tool for various applications in biomedical sciences ranging from cell sorting, cell surface characterization and drug discovery. In this review, we will discuss more specifically how SPR is used to monitor the dynamics of various types of cellular binding events and morphological adherence changes in response to external stimuli.

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Section: Application Of Spr In the Analysis Of Cell Receptor-ligand Imentioning

confidence: 98%

“…Conventional SPR, SPRi and long-range SPR (LRSPR) have each been used for assessing these cellular responses [15,17,[42][43][44]. Reproduced with permission from [39].…”

Section: Application Of Spr In the Analysis Of Cellular Behavior Undementioning

confidence: 98%

Section: Application Of Spr In the Analysis Of Cell Receptor-ligand Imentioning

confidence: 99%

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Surface Plasmon Resonance: a Label-Free Tool for Cellular Analysis

Zeidan

Kepley

Sayes

et al. 2015

Nanomedicine (Lond.)

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“…This approach is mainly based on the specific enzymatic cleavage of target molecules incorporated in the SAM structure [107][108][109]. Of particular interest is the use of enzyme-responsive SAMs for creating cell-based sensors [109] and devices for fast, reliable, and label-free primary cell sorting [110]. Bombera et al [110] have pursued a strategy that used DNA-biochips that could be turned into cell-capturing microarrays by functionalization with antibody-DNA conjugates ( Fig.…”

Section: Samsmentioning

confidence: 99%

Stimuli‐Responsive Surfaces in Biomedical Applications

Pranzetti¹,

Preece²,

Mendes³

2013

Intelligent Stimuli‐Responsive Materials

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Nature provides mechanisms that are able to dynamically control specific and nonspecific interactions between cells and biological surfaces [1,2]. Scientists have long tried to reproduce these dynamic biological events and have recently made an important step in that direction by creating artificial stimuli-responsive surfaces [3][4][5][6][7]. These smart substrates present modulatory surface properties that are able to respond to external chemical/biochemical [8][9][10][11][12], thermal [13][14][15], electrical [16][17][18][19][20], and optical stimuli [21][22][23][24][25][26][27][28][29][30][31]. Due to their dynamic nature such substrates are very appealing for applications in the biomedical field [32]. Progress to date has led to control over biomolecule activity [33] and immobilization of a diverse array of proteins, including enzymes [34] and antibodies [35]. These prior achievements have encouraged researchers to take the challenge of using dynamic surfaces to modulate larger and more complex systems, such as bacteria [36] and mammalian cells [37].Achieving control over surface properties could provide new insights in the understanding of cell behavior and can offer distinct benefits with regard to the development of medical devices. For instance, the modulation of cell attachment and detachment could lead to the prevention of unwanted bacteria fouling on implants, reducing the risk of infections and rejection [38][39][40][41][42]. Furthermore, dynamic surfaces able to present on demand regulatory signals to a cell could provide unprecedented opportunities in studies of cell responses in real-time. Cells in tissues adhere to and interact with their extracellular environment via specialized cell-cell and cell-extracellular

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“…Specific isolation of the cells of interest using antibodies immobilized on a solid surface has been exploited in Cell-Affinity Chromatography (CAC) devices [5]–[9] and protein arrays [10]–[14]. Affinity-based cell capture performed in miniaturized devices has been recently reported, including parallel functionalized microfluidic channels [15], [16] and single microchannels containing several antibody-coated regions [17], [18], antibody-covered micropillars [19], [20] or an antibody-coated porous membrane [21].…”

Section: Introductionmentioning

confidence: 99%

Selective Individual Primary Cell Capture Using Locally Bio-Functionalized Micropores

et al. 2013

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BackgroundSolid-state micropores have been widely employed for 6 decades to recognize and size flowing unlabeled cells. However, the resistive-pulse technique presents limitations when the cells to be differentiated have overlapping dimension ranges such as B and T lymphocytes. An alternative approach would be to specifically capture cells by solid-state micropores. Here, the inner wall of 15-µm pores made in 10 µm-thick silicon membranes was covered with antibodies specific to cell surface proteins of B or T lymphocytes. The selective trapping of individual unlabeled cells in a bio-functionalized micropore makes them recognizable just using optical microscopy.Methodology/Principal FindingsWe locally deposited oligodeoxynucleotide (ODN) and ODN-conjugated antibody probes on the inner wall of the micropores by forming thin films of polypyrrole-ODN copolymers using contactless electro-functionalization. The trapping capabilities of the bio-functionalized micropores were validated using optical microscopy and the resistive-pulse technique by selectively capturing polystyrene microbeads coated with complementary ODN. B or T lymphocytes from a mouse splenocyte suspension were specifically immobilized on micropore walls functionalized with complementary ODN-conjugated antibodies targeting cell surface proteins.Conclusions/SignificanceThe results showed that locally bio-functionalized micropores can isolate target cells from a suspension during their translocation throughout the pore, including among cells of similar dimensions in complex mixtures.

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DNA-directed capture of primary cells from a complex mixture and controlled orthogonal release monitored by SPR imaging

Cited by 27 publications

References 40 publications

Surface Plasmon Resonance: a Label-Free Tool for Cellular Analysis

Surface Plasmon Resonance: a Label-Free Tool for Cellular Analysis

Stimuli‐Responsive Surfaces in Biomedical Applications

Selective Individual Primary Cell Capture Using Locally Bio-Functionalized Micropores

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