Surface Plasmon Resonance: a Label-Free Tool for Cellular Analysis

Abstract

Surface plasmon resonance (SPR) is a popular technique that allows for sensitive, specific, label-free and real-time assessment of biomolecular interactions. SPR is a nondestructive, modular and flexible tool for various applications in biomedical sciences ranging from cell sorting, cell surface characterization and drug discovery. In this review, we will discuss more specifically how SPR is used to monitor the dynamics of various types of cellular binding events and morphological adherence changes in response to external stimuli.

Surface Plasmon Resonance Microscopy: From Single‐Molecule Sensing to Single‐Cell Imaging

“…This label-free optical technique has been commercialized and extensively applied in fundamental biological research, clinical diagnosis,and drug discovery. [5] Although powerful, conventional SPR spectroscopy lacks spatial resolution or imaging capability,hindering its broader applications.W henadigital camera is employed for image acquisition, acquisition of two-dimensional images (termed SPR imaging) is feasible,e ndowing this technique with visualization capability for high-throughput analyses. [6] SPR imaging has since become ar obust analytical tool for multiplexed microarray detection of biomolecular binding events,e specially for in vitro drug screening.…”

Surface Plasmon Resonance Microscopy: From Single‐Molecule Sensing to Single‐Cell Imaging

“…[3] SPRM was derived from surface plasmon resonance (SPR) spectroscopy,w hich has been widely used in studying biomolecular binding kinetics. [5] Although powerful, conventional SPR spectroscopy lacks spatial resolution or imaging capability,hindering its broader applications.W henadigital camera is employed for image acquisition, acquisition of two-dimensional images (termed SPR imaging) is feasible,e ndowing this technique with visualization capability for high-throughput analyses. This label-free optical technique has been commercialized and extensively applied in fundamental biological research, clinical diagnosis,and drug discovery.…”

Label-free, real-time on-chip sensing of living cancer cell via grating-coupled surface plasmon resonance

“…Recently, applications of SPR sensors as label-free and real-time approach for dynamic cellular analysis have been reported with promising results [9]- [11]. Although most application are based on SPR imaging and focused on antigen detection, and live cell dynamic reactions are detectable as angle of resonance of the SPR changes.…”

“…To date, several challenges need to be addressed to promote the application of SPR-based label-free cell analyses, such as the maintenance of cell integrity during the experimental process. Most of commercially available instruments use glass slides coated with gold, that should be installed on the prism before analysis with a careful handling process to reduce cell damage [9]. Moreover, some instruments can tolerate in the microfluidic components only particulates under few microns, thus cell-SPR application would cause clogging [9].…”

“…Most of commercially available instruments use glass slides coated with gold, that should be installed on the prism before analysis with a careful handling process to reduce cell damage [9]. Moreover, some instruments can tolerate in the microfluidic components only particulates under few microns, thus cell-SPR application would cause clogging [9]. We previously developed and optimized a nanostructured sensing platform based on grating-coupling and phase-interrogation [14], [15].…”