DNA Adducts in Perch (Perca fluviatilis) Living in Coastal Water Polluted with Bleached Pulp Mill Effluents

Remediation of lignin and its derivatives from pulp and paper industry wastewater by the combination of chemical precipitation and ozonation

“…The resinic acids are toxic components with securities of 0.4-1.1 mg/L [15]. Many authors reported the presence of toxic species in fish or toxic effects on fish such as respiratory stress, mixed function oxygenase activity, toxicity and mutagenicity, liver damage, or genotoxic effects, and lethal effects on the fishes exposed to pulp and paper mill wastewaters [25][26][27]. Since described before, in certain cases novel treatment technologies or the combination of different alternative methods for the remediation of contaminated water are necessary [18,19,21,23].…”

Ecotoxicology: A Review of Pesticides Induced Toxicity in Fish

“…Various studies conducted have shown that pollutants cause carcinogenesis (Dunn, 1991;Weishburger and Williams, 1991;El Adlouni et al, 1995;Erickson and Larsson, 2000), teratogenesis, clastogenesis and mutation in fish (Obiakor et al, 2012) which ultimately lead to reduced growth, malignancies, reduction in survival of fish in early life stages as well as adult stage, abnormal development and different deformities of body organs (Akpoilih, 2012). There is rich documented literature witnessing research on molecular level of different fish species showing ill effects of pesticides on genes and DNA levels (Stahl, 1991;Helma et al, 1996;Mitchelmore and Chipman, 1998;White et al, 1998a;White et al, 1998b;Hose and Brown, 1998;Hartmann et al, 1999;Gartiser et al, 2001;Vargas et al, 2001;Çavas and Könen, 2007).…”

Polycyclic Aromatic Hydrocarbons a Constituent of Petroleum: Presence and Influence in the Aquatic Environment

“…It has been shown that DNA adducts persisted in vertebrates species due to the low efficiency of repair systems, representing a parameter for long term exposure [174]. These adducts are very persistent in fish liver [175][176][177][178]. French et al [174] observed a steady increase in DNA adduct levels during a chronic exposure of sole (Pleuronectes vetulus) to PAH contaminated sediment for 5 weeks, which were persistent even after a depuration period.…”

“…Briefly, the assay involves DNA purification, digestion to normal and adduct-modified 3'-mononucleotides, removal of normal nucleotides (via enzymatic digestion, solvent extraction or chromatographic methods), 32 P-postlabelling at the 5' position of adducted nucleotides followed by chromatographic separation, detection and quantification (via autoradiography, scintillation counting or phosphor screen imaging analysis) [182]. Following this assay, PAHs cause the appearance of the diagonal radioactive zone (DRZ) (Figure 10) [173,178,183,184]. MS/MS analysis has recently emerged as a powerful tool in the detection and structure elucidation of DNA adducts as well as for their quantification at very low concentrations (as often present in biological samples).…”