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Rao, K. Jagannadha; Kim, Chul Ho; Chung, Bong Hyun; Kim, Myung Kuk; Rhee, Shin Hyung

doi:10.1023/a:1005443306866

Cited by 6 publications

(1 citation statement)

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“…Proteolytic degradation has been an ongoing concern for the production of recombinant proteins in other hosts (Zhou and Zhang 2002), such as P. pastoris (Sinha et al 2005) and S. cerevisiae (Rao et al 1999). On the other hand, a question arises on the stability of plasmid implicated in the production of Xyn2.…”

Section: Validation Of Screening Resultsmentioning

confidence: 99%

Development and validation of a medium for recombinant endo-β-1,4-xylanase production by Kluyveromyces lactis using a statistical experimental design

et al. 2011

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Kluyveromyces lactis is an excellent host for a high cell density culture, which allows high expression levels of recombinant enzymes. Nutrient composition and culture conditions affect the secretion, production level and stability of the recombinant host. Therefore, it is technologically important to formulate a medium that stimulates high cell density and enhances the desired enzyme production using K. lactis GG799. In this study, six media were initially compared, and a Plackett-Burman experimental design was employed to screen for important components and trace elements. Nitrogen sources such as ammonium sulfate and free amino acid (casamino acid) as well as compounds like MgSO 4 •7H 2 O, Na 2 SO 4 , ZnSO 4 •6H 2 O, MnSO 4 •4H 2 O and KH 2 PO 4 affected biomass concentrations (5.67 g/l) and recombinant endo-β-1,4

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Section: Validation Of Screening Resultsmentioning

confidence: 99%