Diversity of cultivable fungal endophytes in Paullinia cupana (Mart.) Ducke and bioactivity of their secondary metabolites

Abstract

Paullinia cupana is associated with a diverse community of pathogenic and endophytic microorganisms. We isolated and identified endophytic fungal communities from the roots and seeds of P. cupana genotypes susceptible and tolerant to anthracnose that grow in two sites of the Brazilian Amazonia forest. We assessed the antibacterial, antitumor and genotoxic activity in vitro of compounds isolated from the strains Trichoderma asperellum (1BDA) and Diaporthe phaseolorum (8S). In concert, we identified eight fungal species not previously reported as endophytes; some fungal species capable of inhibiting pathogen growth; and the production of antibiotics and compounds with bacteriostatic activity against Pseudomonas aeruginosa in both susceptible and multiresistant host strains. The plant genotype, geographic location and specially the organ influenced the composition of P. cupana endophytic fungal community. Together, our findings identify important functional roles of endophytic species found within the microbiome of P. cupana. This hypothesis requires experimental validation to propose management of this microbiome with the objective of promoting plant growth and protection.

Fungal Endophytic Community Associated with Guarana (Paullinia cupana Var. Sorbilis): Diversity Driver by Genotypes in the Centre of Origin

“…In the present study, the susceptible genotype and, in general, the municipality of Manaus, were shown to have greater diversity. Similar results were observed in the seeds and roots of guarana studied in [ 42 ]. The authors related the richness of the susceptible genotype to the host vulnerability to microbial infections.…”

“…The understanding of the factors guiding the microbial community of guarana is extremely important because it can elucidate how these microorganisms are structured in specific niches. In addition, the symbiotic interactions between endophytic fungi and guarana have been little explored [ 42 , 43 , 44 ]. The aim of this study was to identify the taxonomic composition of endophytes on leaves and seeds of guarana ( Paullinia cupana var.…”

Diversity and antimicrobial activity of endophytic fungi isolated from Securinega suffruticosa in the Yellow River Delta

“…control agents that fight the plant pathogens of S. suffruticosa, but also as new secondary metabolite resources with biological activities [63]. Of the 35 endophytic fungi, C. globosum, Fusarium sp.…”

Bioactive secondary metabolites from new endophytic fungus Curvularia. sp isolated from Rauwolfia macrophylla

“…The last endophytic fungus ( Purpureocillium lilacinum ) was among them specifically studied due to its potent antiprotozoal activity against Leishmania donovani (IC 50 0.174 μg mL -1 ); which was attributed to its production of purpureone, an ergochrome moiety [7]. It should be considered that different factors such as the plant organs, genotypic and geographic location influence the endophytic fungal community structure [8]. Endophytic fungi Curvularia sp.…”