Bioactive secondary metabolites from new endophytic fungus Curvularia. sp isolated from Rauwolfia macrophylla

Abstract

Over the last decades, endophytic fungi represent a new source of pharmacologically active secondary metabolites based on the underlying assumption that they live symbiotically within their plant host. In the present study, a new endophytic fungus was isolated from Rauwolfia macrophylla , a medicinal plant from Cameroon. The fungus showed a highest homology to Curvularia sp. based on complete nucleotide sequence data generated from the internal transcribed spacer (ITS) of ribosomal DNA region. Large scale fermentation, working-up and separation of the strain extract using different chromatographic techniques afforded three bioactive compounds: 2'-deoxyribolactone ( 1 ), hexylitaconic acid ( 2 ) and ergosterol ( 3 ). The chemical structures of compounds 1–3 were confirmed by 1 and 2D NMR spectroscopy and mass spectrometry, and comparison with corresponding literature data. Biologically, the antimicrobial, antioxidant activities and the acetylcholinesterase inhibitory of the isolated compounds were studied.

Bioactivity Screening and Identification of Secondary Metabolites from Fungal Endophytes of <i>Carica papaya</i> L. Leaves

“…and Curvularia sp. of several medicinal plants 23,25,26 . So, the isolated fungi have potentiality to produce biologically active compounds for antimicrobial agent development as these previous reports.…”

“…and Alternaria sp. of several medicinal plants 23,25,27,28,30 . However, Colletotrichum gloeosporioides isolated from leaves of C. papaya exhibited moderate antioxidant activity in the DPPH assay and moderate cytotoxic activity against cisplatin-sensitive ovarian cancer cell line 2780 (sens) and cisplatin-resistant ovarian cancer cell line 2780 (CisR) 8 .…”

“…2ˈ-deoxyribolactone and hexylitaconic acid isolated from Curvularia sp. of Rauwolfia macrophylla collected in Cameroon exhibited antibacterial and cytotoxic activities25 . 11-α-methoxycurvularin isolated from Curvularia sp.…”

Evaluation of Antiviral, Antibacterial and Antiproliferative Activities of the Endophytic Fungus Curvularia papendorfii, and Isolation of a New Polyhydroxyacid

“…The extract of endophytic fungus Curvularia sp. T12 isolated from Rauwolfia macrophylla had antibacterial activity against E. coli, Micrococcus luteus, Pseudomonas agarici and S. warneri [48].…”

Strategies to Modulate Specialized Metabolism in Mediterranean Crops: From Molecular Aspects to Field

“…Looking at the plant as an holobiont, attention has been also dedicated to evaluating the role of endophytic fungi in alterations of metabolite production and amounts of active compounds in medicinal plants. In addition, endophytic microbial communities associated with medicinal plants have a great potential as producers of bioactive compounds for agricultural, pharmaceutical, and other industrial applications [ 96 , 97 ]. Recently, SM profiles of endophytic fungi isolated from Salvia abrotanoides originated from three geographically distinct sites in Iran have been evaluated.…”