DIVERSITY AND ABUNDANCE OF POTENTIALLY TOXICPSEUDO-NITZSCHIAPERAGALLO IN AVEIRO COASTAL LAGOON, PORTUGAL AND DESCRIPTION OF A NEW VARIETY,P. PUNGENSVAR.AVEIRENSISVAR. NOV.

Churro, Catarina; Carreira, Cátia; Rodrígues, Francisco J. Ojea; Craveiro, Sandra C.; Calado, António J.; Casteleyn, Griet; Lundholm, Nina

doi:10.1080/0269249x.2009.9705782

Cited by 66 publications

(89 citation statements)

References 45 publications

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“…Both species can be mainly differentiated by examination of the number of striae and fibulae, which usually requires analysis by electron microscopy. Besides its original description, which confirmed its occurrence in Europe and Monterey Bay, California (Lundholm et al, 2006), it was also mentioned in a few studies carried out in the Drake Passage (Ferrario et al, 2004), northeast subarctic Pacific (Marchetti et al, 2008), the coasts of Portugal (Churro et al, 2009) and Malaysian Borneo (Lim et al, 2012a). In our study, it was confined to northern shelf-break waters sampled during spring, with temperatures of 8.1-9.7 C and salinities from 33.7 to 33.9.…”

Section: Discussionmentioning

confidence: 64%

Species occurrence of the potentially toxigenic diatom genus Pseudo-nitzschia and the associated neurotoxin domoic acid in the Argentine Sea

et al. 2017

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Keywords:Amnesic shellfish poisoning South-western Atlantic Pseudo-nitzschia diversity P. australis P. brasiliana Domoic acid A B S T R A C TThe marine diatom genus Pseudo-nitzschia, the major known producer of the neurotoxin domoic acid (DA) responsible for the amnesic shellfish poisoning (ASP) syndrome in humans and marine mammals, is globally distributed. The genus presents high species richness in the Argentine Sea and DA has been frequently detected in the last few years in plankton and shellfish samples, but the species identity of the producers remains unclear. In the present work, the distribution and abundance of Pseudo-nitzschia species and DA were determined from samples collected on two oceanographic cruises carried out through the Argentine Sea ($39-47 S) during summer and spring 2013. Phytoplankton composition was analysed by light and electron microscopy while DA was determined by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). The genus Pseudo-nitzschia was recorded in 71 and 86% of samples collected in summer and spring, respectively, whereas DA was detected in only 42 and 21% of samples, respectively. Microscopic analyses revealed at least five potentially toxic species (P. australis, P. brasiliana, P. fraudulenta, P. pungens, P. turgidula), plus putatively non-toxigenic P. dolorosa, P. lineola, P. turgiduloides and unidentified specimens of the P. pseudodelicatissima complex. The species P. australis showed the highest correlation with DA occurrence (r = 0.55; p < 0.05), suggesting its importance as a major DA producer in the Argentine Sea. In the northern area and during summer, DA was associated with the presence of P. brasiliana, a species recorded for the first time in the Argentine Sea. By contrast, high concentrations of P. fraudulenta, P. pungens and P. turgidula did not correspond with DA occurrence. This study represents the first successful attempt to link toxigenicity with Pseudo-nitzschia diversity and cell abundance in field plankton populations in the south-western Atlantic.

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Section: Discussionmentioning

confidence: 64%

Species occurrence of the potentially toxigenic diatom genus Pseudo-nitzschia and the associated neurotoxin domoic acid in the Argentine Sea

et al. 2017

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“…Within P. pungens, both nuclear rDNA internal transcribed spacer (ITS) and plastid-encoded rbcL sequences have revealed the existence of three distinct clades (I-III), which are also distinguishable by subtle but clear differences in frustule ultrastructure. Although these clades are currently recognized as subspecific taxa (varieties), the observed genetic and morphological discontinuities suggest that these clades behave as independent evolutionary lineages (36), hence justifying their recognition as discrete species-lineages within a planktonic superspecies P. pungens (35,37). To avoid confusion, we refer to these three taxa as clades I-III.…”

mentioning

confidence: 99%

Limits to gene flow in a cosmopolitan marine planktonic diatom

Casteleyn

Leliaert

Backeljau

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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204

175

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The role of geographic isolation in marine microbial speciation is hotly debated because of the high dispersal potential and large population sizes of planktonic microorganisms and the apparent lack of strong dispersal barriers in the open sea. Here, we show that gene flow between distant populations of the globally distributed, bloom-forming diatom species Pseudo-nitzschia pungens (clade I) is limited and follows a strong isolation by distance pattern. Furthermore, phylogenetic analysis implies that under appropriate geographic and environmental circumstances, like the pronounced climatic changes in the Pleistocene, population structuring may lead to speciation and hence may play an important role in diversification of marine planktonic microorganisms. A better understanding of the factors that control population structuring is thus essential to reveal the role of allopatric speciation in marine microorganisms.allopatric speciation | dispersal | marine cosmopolitan planktonic microorganisms | population structure | microsatellites

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“…Interestingly, the strains belonging to the subtropical California Bay (Santa Cruz, NPU41392 and NPU41262 ) branched off into another lineage with one base pair substitution from the ancestral haplotypes, although these were identified before as P. pungens var. cingulata in previous studies (Churro et al 2009). There is only one base pair substitution between Clade II (NWFSC 004, NWFSC 32 and US-115) and Clade III (DABV01, KBH2, P24, FBA2.A11 and FBA1.B2) strains while five base pair substitutions were revealed between Clade III and Clade I (Cn172).…”

Section: Discussionmentioning

confidence: 99%

“…cingulata and Clade I to the nominal variety of P. pungens, while Clade III differs from the other clades. Churro et al (2009) described Clade III as a new variety, P. pungens var. averiensis.…”

Section: Introductionmentioning

confidence: 99%

“…Molecular tools for describing the phylogeny of this genus provoked the discoveries of high degrees of pseudocryptic diversity and several new species during the last decade (e.g. Lundholm et al 2003Lundholm et al , 2006Amato & Montresor 2008;Churro et al 2009;Quijano-Scheggia et al 2009). More recently, the molecular phylogenetic studies of Pseudo-nitzschia pungens revealed three molecular clades: Clades I, II and III (Casteleyn et al 2008).…”

Section: Introductionmentioning

confidence: 99%

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A study on the phylogeny and phylogeography of a marine cosmopolite diatom from the southern Black Sea

Baytut

2013

Oceanological and Hydrobiological Studies

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A single-cell PCR method was applied to Pseudo-nitzschia pungens strains from the southern Black Sea. Based on the aligment set of the LSU D1-D3 region, a Bayesian molecular phylogeny analysis and a parsimony network analysis were used to investigate phylogenetic clades (Clades I-III) in P. pungens and to determine the ancestral clades. The parsimony network analysis also demonstrated that ancestral haplotypes belonged to Clade II, residing around the northeastern Pacific, while Clade I was distributed globally but antitropicaly. According to the findings of this study, the Black Sea strain (Clade III) shows a global phylogeographic pattern.*

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DIVERSITY AND ABUNDANCE OF POTENTIALLY TOXICPSEUDO-NITZSCHIAPERAGALLO IN AVEIRO COASTAL LAGOON, PORTUGAL AND DESCRIPTION OF A NEW VARIETY,P. PUNGENSVAR.AVEIRENSISVAR. NOV.

Cited by 66 publications

References 45 publications

Species occurrence of the potentially toxigenic diatom genus Pseudo-nitzschia and the associated neurotoxin domoic acid in the Argentine Sea

Species occurrence of the potentially toxigenic diatom genus Pseudo-nitzschia and the associated neurotoxin domoic acid in the Argentine Sea

Limits to gene flow in a cosmopolitan marine planktonic diatom

A study on the phylogeny and phylogeography of a marine cosmopolite diatom from the southern Black Sea

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