Diterpenoids and triterpenoids in hairy roots of Salvia sclarea

Abstract

Hairy root culture of Salvia sclarea L. was established following infection with Agrobacterium rhizogenes LBA 9402. The culture was grown in growth regulator-free half-strength B5 Gamborg medium with 30 g l )1 sucrose and was investigated with respect to its capability of producing diterpenoids and triterpenoids. Four diterpenoids (ferruginol, salvipisone, aethiopinone and 1-oxoaethiopinone) and two ursenetype triterpenoids (2a,3a-dihydroxy-urs-12-en-28-oic acid and 2a,3a,24-trihydroxy-urs-12-en-28-oic acid) were isolated from the hairy roots. The presence of three sterols (b-sitosterol, stigmasterol and campesterol), as well as oleanolic and ursolic acids was also shown by GC-MS analysis. The quantitative and qualitative differences in diterpenoid and triterpenoid production patterns between hairy roots grown in the light and in the dark were described.

Diterpenoid Production in Hairy Root Culture of Salvia sclarea L.

“…The transformed root culture of S. sclarea was obtained through infection of sterile shoots with Agrobacterium rhizogenes (LBA 9402) (see Kuźma et al, 2006). For the time course, the hairy roots were cultured for 50 d, and three flasks were collected every five d during each of three successive subcultures.…”

“…Recently, Agrobacterium rhizogenes-mediated hairy roots of Salvia sclarea were established (Kuźma et al, 2006). The genetically modified root cultures have several advantages.…”

“…They usually demonstrate genetic and biochemical stability, fast growth in hormone-free media and the ability to synthesize root-derived secondary metabolites (Giri and Narasu, 2000). From a S. sclarea hairy root culture, four diterpenoids, namely salvipisone, ferruginol, aethiopinone and 1-oxoaethiopinone, were isolated (Kuźma et al, 2006) (Fig. 1).…”

Establishment of Salvia officinalis L. Hairy Root Cultures for the Production of Rosmarinic Acid

“…The hairy roots were studied for their ability to synthesize RA under light and dark conditions. So far, out of other members of the Salvia genus transformation procedure was used only for S. miltiorrhiza (Chen et al, 1999) and S. sclarea (Kuźma et al, 2005).…”

Trichome Specific Expression: Promoters and Their Applications

“…A number of genes encoding proteins with clear similarities to terpene synthases and enzymes isoprenoid metabolism could be identified, thus providing genes with potentially highly specific pattern of expression, notably restricted to trichome glandular cells. Although transformation and regeneration of transgenic Salvia sclarea plants has not been achieved to date, hairy root cultures were established (Kuzma et al, 2006;Kuzma et al, 2008), and transformation of a related species (Salvia miltiorrhiza) by Agrobacterium tumefaciens could be successfully demonstrated (Yan and Wang, 2007;Lee et al, 2008). These results suggest that trichome specific metabolic engineering of clary sage is technically feasible.…”