Trichome Specific Expression: Promoters and Their Applications

Tissier, Alain

doi:10.5772/32101

Cited by 25 publications

(26 citation statements)

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“…mint), the combination of transcriptomics at different stages of trichome development (see below) and of genome-editing or RNA interference technology should be a powerful approach to address the function of candidate genes. Extensive glandular trichome-specific EST resources were generated for a variety of (nonmodel) plant species (Dai et al, 2010;Tissier, 2012b;Soetaert et al, 2013;Chen et al, 2014;Jin et al, 2014;Trikka et al, 2015;Akhtar et al, 2017). Dedicated trichome-related openaccess resources exist that help researchers mine this vast and increasing amount of trichome-related data.…”

Section: A Detailed Understanding Of Glandular Trichome Initiation Anmentioning

confidence: 99%

Plant Glandular Trichomes: Natural Cell Factories of High Biotechnological Interest

2017

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ORCID IDs: 0000-0001-9302-405X (A.H.); 0000-0002-2315-6900 (M.B.); 0000-0002-3688-7614 (C.H.).Multicellular glandular trichomes are epidermal outgrowths characterized by the presence of a head made of cells that have the ability to secrete or store large quantities of specialized metabolites. Our understanding of the transcriptional control of glandular trichome initiation and development is still in its infancy. This review points to some central questions that need to be addressed to better understand how such specialized cell structures arise from the plant protodermis. A key and unique feature of glandular trichomes is their ability to synthesize and secrete large amounts, relative to their size, of a limited number of metabolites. As such, they qualify as true cell factories, making them interesting targets for metabolic engineering. In this review, recent advances regarding terpene metabolic engineering are highlighted, with a special focus on tobacco (Nicotiana tabacum). In particular, the choice of transcriptional promoters to drive transgene expression and the best ways to sink existing pools of terpene precursors are discussed. The bioavailability of existing pools of natural precursor molecules is a key parameter and is controlled by so-called cross talk between different biosynthetic pathways. As highlighted in this review, the exact nature and extent of such cross talk are only partially understood at present. In the future, awareness of, and detailed knowledge on, the biology of plant glandular trichome development and metabolism will generate new leads to tap the largely unexploited potential of glandular trichomes in plant resistance to pests and lead to the improved production of specialized metabolites with high industrial or pharmacological value.

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Section: A Detailed Understanding Of Glandular Trichome Initiation Anmentioning

confidence: 99%

Plant Glandular Trichomes: Natural Cell Factories of High Biotechnological Interest

2017

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“…Overexpression of a PPO from potato ( Solanum tuberosum ) in cultivated tomato yielded transgenic plants that were much more resistant to the bacterial pathogen Pseudomonas syringae [143,144], and in dandelion ( Taraxacum officinale ) suppression of PPO-2 via silencing increased plant susceptibility to P. syringae [145]. Glandular trichomes may also actively secrete proteins, as shown in cultivated tobacco, where proteins can be deposited on the leaf surface through pores that are present in the cuticle of short glandular trichomes [37,146] which are reminiscent of tomato type VII trichomes [147]. These secreted proteins, termed tobacco phylloplanins, inhibited spore germination and leaf infection by the oomycete pathogen Peronospora tabacina [37].…”

Section: Biosynthesis and Function Of Glandular Trichome-produced mentioning

confidence: 99%

“…The preparation of trichome-specific EST databases did not only facilitate the discovery and characterization of genes in trichome biosynthetic pathways, but also made it more feasible to engineer the production of specific biocides in trichomes [147]. Most engineering strategies are not designed to obtain expression of a transgene exclusively in a target tissue, such as a trichome, and/or at specific moments, but make use of a construct in which the transgene is fused behind the general 35S promoter and is expressed either via stable transformation [15] or via virus-induced gene silencing [121,162].…”

Section: Trichome Engineering To Increase Plant Resistancementioning

confidence: 99%

“…Cultivated tobacco and N. tomentosiformis produce both labdanoids and cembranoids, while N. sylvestris only produces the latter group of compounds. These diterpenes are produced in large amounts and, specifically, in the glandular capitate trichomes of the plant, and some of these labdanoids contribute to plant resistance to pests, making tobacco an ideal target for terpenoid metabolic engineering [147]. For example, in cultivated tobacco, downregulation of a trichome-specific CYP450, a CBT-ol hydroxylase, via antisense suppression, led to reduction of CBT-diol levels but promoted the levels of its insecticidal precursor CBT-ol, thereby increasing plant resistance to the red aphid ( Myzus nicotianae ) [164].…”

Section: Trichome Engineering To Increase Plant Resistancementioning

confidence: 99%

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Plant Glandular Trichomes as Targets for Breeding or Engineering of Resistance to Herbivores

Glas¹,

Schimmel²,

Alba³

et al. 2012

IJMS

450

364

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Glandular trichomes are specialized hairs found on the surface of about 30% of all vascular plants and are responsible for a significant portion of a plant’s secondary chemistry. Glandular trichomes are an important source of essential oils, i.e., natural fragrances or products that can be used by the pharmaceutical industry, although many of these substances have evolved to provide the plant with protection against herbivores and pathogens. The storage compartment of glandular trichomes usually is located on the tip of the hair and is part of the glandular cell, or cells, which are metabolically active. Trichomes and their exudates can be harvested relatively easily, and this has permitted a detailed study of their metabolites, as well as the genes and proteins responsible for them. This knowledge now assists classical breeding programs, as well as targeted genetic engineering, aimed to optimize trichome density and physiology to facilitate customization of essential oil production or to tune biocide activity to enhance crop protection. We will provide an overview of the metabolic diversity found within plant glandular trichomes, with the emphasis on those of the Solanaceae, and of the tools available to manipulate their activities for enhancing the plant’s resistance to pests.

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“…, 2009). For more detailed information on trichome‐specific promoters, readers are referred to a recent review on the topic (Tissier, 2012).…”

Section: Glandular Trichomes: Secreting and Producing Glandsmentioning

confidence: 99%

Glandular trichomes: what comes after expressed sequence tags?

2012

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SUMMARYGlandular trichomes cover the surface of many plant species. They exhibit tremendous diversity, be it in their shape or the compounds they secrete. This diversity is expressed between species but also within species or even individual plants. The industrial uses of some trichome secretions and their potential as a defense barrier, for example against arthropod pests, has spurred research into the biosynthesis pathways that lead to these specialized metabolites. Because complete biosynthesis pathways take place in the secretory cells, the establishment of trichome-specific expressed sequence tag libraries has greatly accelerated their elucidation. Glandular trichomes also have an important metabolic capacity and may be considered as true cell factories. To fully exploit the potential of glandular trichomes as breeding or engineering objects, several research areas will have to be further investigated, such as development, patterning, metabolic fluxes and transcription regulation. The purpose of this review is to provide an update on the methods and technologies which have been used to investigate glandular trichomes and to propose new avenues of research to deepen our understanding of these specialized structures.

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Trichome Specific Expression: Promoters and Their Applications

Cited by 25 publications

References 142 publications

Plant Glandular Trichomes: Natural Cell Factories of High Biotechnological Interest

Plant Glandular Trichomes: Natural Cell Factories of High Biotechnological Interest

Plant Glandular Trichomes as Targets for Breeding or Engineering of Resistance to Herbivores

Glandular trichomes: what comes after expressed sequence tags?

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