Distribution of vacA alleles and cagA status of Helicobacter pylori in peptic ulcer disease and non-ulcer dyspepsia

Aydın, Faruk; Kaklıkkaya, Neşe; Özgür, Orhan; Çubukçu, Kıvanç; Kılıç, Ali; Tosun, İlknur; Ertürk, Murat

doi:10.1111/j.1469-0691.2004.00989.x

Cited by 23 publications

(28 citation statements)

References 22 publications

(28 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, in the present study we investigated the cagA prevalence and vacA allelic subtypes retrospectively and evaluated their relationships with clinical outcomes in strains that had been isolated at Hacettepe University Hospital. Table 5 summarises the vacA s and m genotype rates in the present study and in other studies from Turkey (7,(12)(13)(14)(15)(16)(17). It has been known that cagA positivity rates and their association with clinical outcomes differ from region to region.…”

Section: Resultsmentioning

confidence: 92%

Detection of cagA and vacA genotypes of Helicobacter pylori isolates from a university hospital in Ankara region, Turkey

Erdoğdu

Sarıbaş²,

Yilmaz³

2014

Turk J Med Sci

View full text Add to dashboard Cite

The cagA and vacA profiles and their association with clinical findings show a distinct geographical distribution. In the present study, we aimed to determine the cagA status and vacA allelic subtypes in strains isolated from a university hospital in Ankara and to evaluate their associations with histopathological and endoscopic findings. Materials and methods:A total of 120 H. pylori strains from stock cultures positive for the ureA gene were randomly included in the present study. Of these strains, cagA and vacA allelic subtypes (s1a, s1b, s2, m1, m2) were examined by polymerase chain reaction.Results: Of the 120 strains, 64 (53.3%) were cagA-positive. However, no significant relationship was found between clinical outcomes and cagA positivity. There were 38 (33.6%) strains that had vacA m1 and 74 (65.5%) that had vacA m2 region. Overall, 75 (70.1%) samples were classified as vacA s1a, 3 (2.8%) as vacA s1b, and 29 (27.1%) as vacA s2. There was no significant relationship between vacA genotypes and endoscopic findings. The predominant vacA genotypes were s1am2 (35.6%) and s1am1 (33.6%), with almost the same rates. Furthermore, cagA positivity was found to be significantly related with the vacA s1am1 genotype. Conclusion:The cagA and vacA profiles of our study population are consistent with the Middle Eastern profile.

show abstract

Section: Resultsmentioning

confidence: 92%

Detection of cagA and vacA genotypes of Helicobacter pylori isolates from a university hospital in Ankara region, Turkey

Erdoğdu

Sarıbaş²,

Yilmaz³

2014

Turk J Med Sci

View full text Add to dashboard Cite

show abstract

“…The prevalence of vacA s1m1 genotype in our study was 6.6% and 25% of the strains in patients with CG and DU, respectively. The assesment of vacA gene mosaicism found only three out of the four possible combinations, the s2m1 mosaicism being never detected in our series, which was typically observed in strains from Western country including Turkey [18,19,22,24] . In this study, the cagA vacA s1m2 genotype was predominant genotype, and there was no significant relationship between both of the pathologies, indicating that the genotype was common virulence factors of H pylori.…”

Section: Discussionmentioning

confidence: 90%

“…The vacA s1m2 genotype predominant irrespective of the clinical outcome was also predominant in strains from Western countries including Turkey [4,18,22,27] . Aydin F et al [19] reported the vacA s1m1 genotype was the common vacA genotype in Black sea region located in Asian part of Turkey. The prevalence of vacA s1m1 genotype in our study was 6.6% and 25% of the strains in patients with CG and DU, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…The iceA2 positive strains have been reported more prevalant among patients with non-ulcer dyspepsia while H pylori strains possess iceA1 allele have been more prevalant in peptic ulcer disease [5,17] . The previous studies focused on determining the genotype profiles of H pylori isolates in different clinical outcomes of H pylori infection in Turkey were limited and carried out in different geographic regions which has different environmental factors, and nutritional habits, together with different lifestyle [8,[18][19][20] . Therefore, the aims of this study were to characterize and to compare the genotype profiles of H pylori isolates in patients with chronic gastritis and duodenal ulcer in the western part of Turkey.…”

Section: Rapid Communicationmentioning

confidence: 99%

See 1 more Smart Citation

H pylori iceAalleles are disease-specific virulence factors

Caner

Yılmaz²,

Yönetçi³

et al. 2007

WJG

View full text Add to dashboard Cite

AIM:To characterize and compare genotype profiles of H pylori strains isolated from patients with chronic gastritis and duodenal ulcer in western part of Turkey. METHODS:A total of 46 patients [30 chronic gastritis (CG) and 16 duodenal ulcer (DU)] who had undergone endoscopy because of dyspeptic complaints were studied. The antral biopsy specimens were evaluated for the presence of H pylori by rapid urease test and culture, and the genotype profiles were determined by real-time PCR. RESULTS:The cagA gene was observed in 43 (93.5%) isolates. The vacA s1m2 genotype was the predominant subtype, found in 63.3% and 68.7% of isolates in patients with CG and DU, respectively. Twenty (66.6%) isolates from patients with CG were iceA2 positive while the iceA1 was predominant in those with DU (68.8%). In terms of the association of the iceA alleles to other genes, both alleles were significantly associated with the cagA vacA s1m2 genotype. CONCLUSION:The prevalent circulating genotypes in CG and DU were cagA vacA s1m2 iceA2 and cagA vacA s1m2 iceA1 genotype, respectively. It was found that cagA vacA s1m2 genotype seems to be common virulence factors in both CG and DU while iceA alleles show specificity for gastroduodenal pathologies in this study.

show abstract

“…Recent studies have shown that cagA gene-positive (cagA + ) H. pylori strains cause more severe gastric infections (Aydin et al 2004;Yamazaki et al 2005). However, to the best of our knowledge, there is only one study about cagA + in tonsil and adenoid tissues.…”

confidence: 89%