Distribution of LDH Isozymes in Human Skeletal Muscle

Karlsson, Jan; Frith, Kathleen; Sjödin, B.; Gollnick, P. D.; Saltin, Bengt

doi:10.3109/00365517409082500

Cited by 38 publications

(4 citation statements)

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“…Furthermore, fast (glycolytic) muscle fibers showed hypermethylation of genes that enhance the reliance on aerobic metabolism, such as the slow calcium pump (SERCA2) and the lactate dehydrogenase B (LDH-H). These genes are reported to be less expressed in fast muscle fibers (33,37,58,85,87). Most of these metabolic genes (PFKM, ALDOA, UCP3, SERCA2, and LDH-H) demonstrated hypermethylated CpG sites in gene bodies (Table 3).…”

Section: Cpg Islands 1%mentioning

confidence: 99%

DNA methylation assessment from human slow- and fast-twitch skeletal muscle fibers

Begue

Raue

Jemioło

et al. 2017

Journal of Applied Physiology

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A new application of the reduced representation bisulfite sequencing method was developed using low-DNA input to investigate the epigenetic profile of human slow- and fast-twitch skeletal muscle fibers. Successful library construction was completed with as little as 15 ng of DNA, and high-quality sequencing data were obtained with 32 ng of DNA. Analysis identified 143,160 differentially methylated CpG sites across 14,046 genes. In both fiber types, selected genes predominantly expressed in slow or fast fibers were hypomethylated, which was supported by the RNA-sequencing analysis. These are the first fiber type-specific methylation data from human skeletal muscle and provide a unique platform for future research. This study validates a low-DNA input reduced representation bisulfite sequencing method for human muscle biopsy samples to investigate the methylation patterns at a fiber type-specific level. These are the first fiber type-specific methylation data reported from human skeletal muscle and thus provide initial insight into basal state differences in myosin heavy chain I and IIa muscle fibers among young, healthy men.

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Section: Cpg Islands 1%mentioning

confidence: 99%

DNA methylation assessment from human slow- and fast-twitch skeletal muscle fibers

Begue

Raue

Jemioło

et al. 2017

Journal of Applied Physiology

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“…'Total muscle LDH activity as well as the activity of LDH isozymes in the biopsy material were determined according to Karlsson et al (1974a), respectively. The methodological errors are reported to be less than 20; for determining total LDH activity (Karlsson et a/.…”

Section: Biocheniical and Irisrochentical Ar;alysesmentioning

confidence: 99%

Effect of Physical Training on LDH Activity and LDH Isozyme Pattern in Human Skeletal Muscle

Sjödin

Thorstensson

Frith

et al. 1976

Acta Physiologica Scandinavica

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Total lactate dehydrogenase (LDH) activity and LDH isozyme pattern were studied in muscle biopsies obtained from m. vastus lateralis after 1) "aerobic" training performed as interval and extreme distance running, respectively (3 subjects); and 2) "anaerobic" training for two months, carried out as repeated maximal bursts of approximately 1 min running (6 subjects). After the "anaerobic training" no changes in LDH properties could be detected, although running performance improved. The extreme distance running resulted in a decrease in total LDH activity and an increase in relative activity of the heart specific isozymes. A relationship was also shown between the relative activity of these isozymes and the training distance covered. The relatively more aerobic prevailing during distance running as compared to "anaerobic training" were proposed to decrease muscle specific subunits and/or increase synthesis of heart specific subunits in both muscle fiber types. This suggestion was supported by isozyme analysis of lyophilized and dissected single muscle fibres.

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“…It has been previously reported that the lactate dehydrogenase (LDH-1) subunit, which preferentially catalyzes the conversion of L-lactate to pyruvate, is found in neurons and astrocytes [ 11 , 12 ]. However, LDH-5, which is more highly expressed in astrocytes, preferentially favors the conversion of pyruvate back to L-lactate [ 11 ]; LDH-5 is also present in glycolytic tissues such as skeletal muscles [ 13 , 14 ]. This cell-type distribution of LDH subunits indicates a close link with characteristic features of L-lactate metabolism, which has implications for neuronal development, growth, and survival [ 6 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Participation of L-Lactate and Its Receptor HCAR1/GPR81 in Neurovisual Development

Laroche

Stil

Germain

et al. 2021

Cells

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During the development of the retina and the nervous system, high levels of energy are required by the axons of retinal ganglion cells (RGCs) to grow towards their brain targets. This energy demand leads to an increase of glycolysis and L-lactate concentrations in the retina. L-lactate is known to be the endogenous ligand of the GPR81 receptor. However, the role of L-lactate and its receptor in the development of the nervous system has not been studied in depth. In the present study, we used immunohistochemistry to show that GPR81 is localized in different retinal layers during development, but is predominantly expressed in the RGC of the adult rodent. Treatment of retinal explants with L-lactate or the exogenous GPR81 agonist 3,5-DHBA altered RGC growth cone (GC) morphology (increasing in size and number of filopodia) and promoted RGC axon growth. These GPR81-mediated modifications of GC morphology and axon growth were mediated by protein kinases A and C, but were absent in explants from gpr81−/− transgenic mice. Living gpr81−/− mice showed a decrease in ipsilateral projections of RGCs to the dorsal lateral geniculate nucleus (dLGN). In conclusion, present results suggest that L-lactate and its receptor GPR81 play an important role in the development of the visual nervous system.

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Distribution of LDH Isozymes in Human Skeletal Muscle

Cited by 38 publications

References 0 publications

DNA methylation assessment from human slow- and fast-twitch skeletal muscle fibers

DNA methylation assessment from human slow- and fast-twitch skeletal muscle fibers

Effect of Physical Training on LDH Activity and LDH Isozyme Pattern in Human Skeletal Muscle

Participation of L-Lactate and Its Receptor HCAR1/GPR81 in Neurovisual Development

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