Total lactate dehydrogenase (LDH) activity and LDH isozyme pattern were studied in muscle biopsies obtained from m. vastus lateralis after 1) "aerobic" training performed as interval and extreme distance running, respectively (3 subjects); and 2) "anaerobic" training for two months, carried out as repeated maximal bursts of approximately 1 min running (6 subjects). After the "anaerobic training" no changes in LDH properties could be detected, although running performance improved. The extreme distance running resulted in a decrease in total LDH activity and an increase in relative activity of the heart specific isozymes. A relationship was also shown between the relative activity of these isozymes and the training distance covered. The relatively more aerobic prevailing during distance running as compared to "anaerobic training" were proposed to decrease muscle specific subunits and/or increase synthesis of heart specific subunits in both muscle fiber types. This suggestion was supported by isozyme analysis of lyophilized and dissected single muscle fibres.
Distribution of LDH Isozymes in Human Skeletal Muscle. Scand. J. d i n . Lab. Invest. 33,LDH isozyme patterns in human skeletal muscle have been studied in 17 subjects of both sexes and varying age groups (16-55 years). Homogenized muscle biopsy materials have been analysed for total LDH activity (V,,,,) in both directions, as well as enzyme activity attributable to isozymes. In addition, different isozymes have been electrophoretically separated and stained. In human skeletal muscle all (5) LDH isozymes were present, although the relative contribution from the more heart-specific isozymes (LDH 1 plus 2) declined the higher the total LDH activity. In absolute terms. however, this decline corresponded to a 2 to 3 fold increase in the activity of LDH 1 plus 2 when the two extremes with low and high total LDH activity. respectively, were compared. Per cent LDH 1 plus 2 of total LDH activity was found to increase with an increase in per cent slow-twitch fibres. As an increase in total LDH activity was synonymous to an increase in per cent fast-twitch fibres, it was concluded that LDH isozyme pattern in human skeletal muscle was related to the fibre type. Moreover, the slow-twitch fibre had a relatively higher activity of the isozyme fractions corresponding to the more heart-specific isozymes LDH 1 and LDH 2. -och idrottshogskolan. Lidiugiivagen I . S-114 33 Stockholm, Sweden 307-3 12,1974. Scand J Clin Lab Invest Downloaded from informahealthcare.com by Chulalongkorn University on 01/02/15 For personal use only. Scand J Clin Lab Invest Downloaded from informahealthcare.com by Chulalongkorn University on 01/02/15 For personal use only.
Muscle biopsy samples were obtained from arm and leg muscles of endurance and strength trained athletes, respectively. Total LDH activity as well as occurrence and activity of LDH isozymes were determined. Comparing the results from the athletes with those from non-trained subjects with corresponding fibre compositions, it was found that the endurance athletes had a lower total LDH activity, a higher relative activity of the most heart-specific isozymes. LDH (1 + 2), and, on electrophoretic separation, a complete absence of LDH (4 + 5) in both arm and leg muscles. As compared to the untrained material the strength trained athletes tended to have a higher total LDH activity, a similar distribution of relative isozyme activities, and, in the leg muscles, a strong electrophoretic band corresponding to LDH 5, the most skeletal muscle specific isozyme.
Case reportgave her an expected date of delivery of 10 October 1987. The antenatal follow up was uncomplicated until 39 weeks when she developed a mild hypertension with systolic/diastolic blood presure of 160/90 mmHg. She was A 33 year old, healthy Primigravida booked at l1 weeks Of S3 7RE.asymptomatic and there was no proteinuria. Her blood pressure settled with bedrest, but a decision was made to induce labour at term.
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