Distribution of Epithelial Membrane Antigen in Eccrine Poroma

Abstract

Using immunohistochemical methods, we investigated the distribution of epithelial membrane antigen (EMA) on the normal eccrine gland, eccrine poroma and hidroacanthoma simplex. Granular membrane-associated reaction of EMA was detected on the outer cells of both the intraepidermal and the upper portion of intradermal eccrine ducts, as well as on the luminal surfaces and intercellular canaliculi of eccrine glands. Clear immunolabeling was also present in the tumor cells of eccrine poroma and hidroacanthoma simplex. Thus it is suggested that the constituent cells of these tumors originate from the outer cells of the intraepidermal and/or the upper portion of the intradermal eccrine ducts. There was no immunolabeling for EMA on the tumor cells of seborrheic keratosis and basal cell carcinoma. Immunohistochemical staining for EMA is a useful tool for the diagnosis of skin appendage tumors.

Malignant Hidroacanthoma Simplex: A Case Report

“…EMA and CEA have been reported to be helpful in diagnosing eccrine poroma (11,12), but this is not supported by the report of Perniciaro et al (10). In our case, EMA was positive in only one focus of tumor cells; the rest were not stained.…”

A Single Lesion Demonstrating Features of Eccrine Poroma and Poroid Hidradenoma

“…Recent immunohistochemical analysis of keratin has revealed that the poroid cells in eccrine poroma are differentiated toward the peripheral cells of dermal eccrine ducts (23). Poroid cells closely resemble the eccrine duct peripheral cells from the lower intraepidermal to uppermost dermal segment (4), which are reported to be immunohistochemically positive for EMA (24). Cuticular cells resemble the eccrine duct luminal cells from the lower intraepidermal to dermal segment (4); these cells are, at least in our case, rich in the poroid hidradenoma region.…”

An immunohistochemical study of the origin of the solid strand in syringoma, using carcinoembryonic antigen, epithelial membrane antigen, and cytokeratin 5

“…Ohnishi and Watanabe 5 reported specifically that EMA was clearly found in the acrosyringium of normal eccrine glands, the luminal cells of the dermal duct, and the luminal surface of conventional syringoma and clear cell syringoma. On the other hand, some papers claimed that the granular membrane‐associated reaction of EMA was detected on the outer cells of both the acrosyringium and upper portion of intradermal eccrine ducts, as well as on the luminal surfaces and intercellular canaliculi of eccrine glands 2,3,20 . Although we did not perform experiments regarding EMA expression in the normal sweat glands for this study, from a total of 31 cases, 30 (96.8%) were stained in the outer cells of the ductal structure of the syringomas, and 29 (93.5%) in the solid strands, whereas only two (6.5%) were stained in the inner cells of the ductal structure.…”

“…On the other hand, some papers claimed that the granular membraneassociated reaction of EMA was detected on the outer cells of both the acrosyringium and upper portion of intradermal eccrine ducts, as well as on the luminal surfaces and intercellular canaliculi of eccrine glands. 2,3,20 Although we did not perform experiments regarding EMA expression in the normal sweat glands for this study, from a total of 31 cases, 30 (96.8%) were stained in the outer cells of the ductal structure of the syringomas, and 29 (93.5%) in the solid strands, whereas only two (6.5%) were stained in the inner cells of the ductal structure. This leads us to believe that the solid strands originate from the intraepidermal duct and from the outer cells of the upper portion of the intradermal sweat duct.…”