1996
DOI: 10.1113/jphysiol.1996.sp021630
|View full text |Cite
|
Sign up to set email alerts
|

Distinct voltage‐dependent gating behaviours of a swelling‐activated chloride current in human epithelial cells.

Abstract: 1. The swelling-activated chloride current is critical in the homeostatic regulatory volume decrease (RVD) of both excitable and non-excitable cells. Although not activated by voltage, it displays kinetic behaviour similar to voltage-gated Shaker-type potassium currents. We have studied the voltage-dependent properties of this current in single T84 human cell line epithelial cells using whole-cell patch clamp methodology. 2. An external anion permeability sequence of I-> Cl-> methanesulphonate (MeSO3) was obse… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

3
12
0

Year Published

1997
1997
2014
2014

Publication Types

Select...
7

Relationship

0
7

Authors

Journals

citations
Cited by 14 publications
(15 citation statements)
references
References 32 publications
(50 reference statements)
3
12
0
Order By: Relevance
“…Biophysical differences include lack of inactivation and a smaller single‐channel conductance. Most I Clswell exhibit voltage‐sensitive inactivation (Jackson & Strange, 1995a; Nilius et al ., 2003), although the rate and degree vary (Jentsch et al ., 2002), and may depend on extracellular Mg 2+ (Braun & Schulman, 1996; Voets et al ., 1997). Microglia lack inactivation, even at strongly depolarized potentials, and with the highest divalent cation concentration used in previous I Clswell studies.…”
Section: Discussionsupporting
confidence: 80%
See 1 more Smart Citation
“…Biophysical differences include lack of inactivation and a smaller single‐channel conductance. Most I Clswell exhibit voltage‐sensitive inactivation (Jackson & Strange, 1995a; Nilius et al ., 2003), although the rate and degree vary (Jentsch et al ., 2002), and may depend on extracellular Mg 2+ (Braun & Schulman, 1996; Voets et al ., 1997). Microglia lack inactivation, even at strongly depolarized potentials, and with the highest divalent cation concentration used in previous I Clswell studies.…”
Section: Discussionsupporting
confidence: 80%
“…;3 Eder et al (1998);4 Jackson et al (1995b); 5Boese et al (1996); 6 Jentsch et al (2002); 7 d'Anglemont de Tassigny et al (2003); 8 Nilius et al (2003); 9 Sardini et al (2003); 10 Lewis et al (1993); 11Schumacher et al (1995);12 Nilius et al (1998);13 Braun et al (1996); 14Voets et al (1997);15 Meyer et al (1996);16 Wang et al (2005). *The negative sign ()0.10) indicates an increase in volume.…”
mentioning
confidence: 99%
“…The estimated values relative to Clwere 1.21 ± 0.09 for iodide, and 0.43 ± 0.07 for aspartate. The estimate for iodide is in good agreement with relative values determined in recent studies on human epithelial cells (1.37, Braun and Schulman 1996) and guinea-pig atrial and ventricular cells (~1.1, calculated from data in Fig. 2 of Duan et al 1999), whereas the estimate for aspartate is similar to the 0.45 value reported for anion current carried by ClC-3 channels expressed in NIH/3T3 cells (Duan et al 1997).…”
Section: Relative Permeability and Conductancesupporting
confidence: 89%
“…Clcurrent through this outwardly-rectifying channel (I Cl,Vol ) inactivates during depolarizations to high positive potentials (Duan et al 1997;Duan et al 1999), a property that has been described for I Cl,Vol in a wide variety of cell types, in-cluding T lymphocytes (Lewis et al 1993), epithelium (Worrell et al 1989;Kubo and Okada 1992;Braun and Schulman 1996), endothelium (Nilius et al 1994), osteoblast-like cells (Gosling et al 1995), and various cancer-cell lines (Anderson et al 1995).…”
Section: Introductionmentioning
confidence: 93%
“…Of note is that NPPB and niflumic acid appeared to produce a voltage-independent block of both outward and inward currents, whereas DIDS appeared to produce predominantly a voltage-dependent block of outward currents only. Such a voltage-dependent block by DIDS has been demonstrated previously for swelling-activated chloride currents in other cell types (Braun and Schulman, 1996;Ehring et al, 1994;Winpenny et al, 1996;Mitchell et al, 1997). We compared the percentage inhibition by each test drug in Figure 4D,E.…”
Section: Chloride Channel Blockers Inhibit the Hypotonically Activatementioning
confidence: 99%