Distinct H₂O₂concentration promotes proliferation of tumour cells after transient oxygen/glucose deprivation

Abstract

A solid tumour undergoes ischemia/reperfusion due to deficient vascularization and subsequent formation of new blood vessels. This study investigated the effect of transient oxygen and glucose deprivation (OGD) on proliferation of C6 glioma cells. The cells were subjected to 18 h of OGD followed by reoxygenation in the presence of glucose and different extra-cellular H(2)O(2) concentrations since H(2)O(2) affects cell proliferation. After reoxygenation, the cellular H(2)O(2) concentration was increased returning to control levels within 24 h. Within this period, increase in cell number and MTT-reduction were impaired. Regeneration was completed on the third day of reoxygenation. MTT-reduction increased faster than cell number, indicating an OGD-dependent up-regulation of protein expression. It is concluded that ischemia/reperfusion stress promotes proliferation of tumour cells. An essential factor is a distinct H(2)O(2) concentration. Massive elevation as well as significant reduction of H(2)O(2) concentration impairs the proliferation process.

Genetic oxidative stress variants and glioma risk in a Chinese population: a hospital-based case–control study

“…The overexpression of SOD2 can also induce increased levels of hydrogen peroxide (H2O2) [40,41]. H2O2 is a major intracellular oxidant and induces DNA damage in glioma cells [42,43]. Although it might be difficult to determine the precise mechanisms that are most relevant to the pathologies of the patients in this study, the identification of these two possible mechanisms is consistent with our results.…”

Endogenous Nitric Oxide Generation Linked to Ryanodine Receptors Activates Cyclic GMP / Protein Kinase G Pathway for Cell Proliferation of Neural Stem/Progenitor Cells Derived From Embryonic Hippocampus

“…Besides their well-known toxic effects, ROS/NO could be responsible for the modulation of various cellular functions under normal conditions (26). Recent reports demonstrated that ROS play an important role in proliferation of proliferative cells (27,28). Using ROS scavengers and an NADPH oxidase inhibitor, our previous study showed that proliferative activity is positively regulated by ROS, which are endogenously generated by activation of NADPH oxidase throughout the cell proliferation of embryonic hippocampal NPCs (15).…”

Advances in antitumor nanomedicine based on functional metal–organic frameworks beyond drug carriers

“…Therapeutic treatments based on adjusting the intracellular H 2 O 2 levels have been investigated. [114][115][116] In this subsection, we focus on therapeutic approaches based on MOFs that are responsive to H 2 O 2 in tumors.…”