Distention ulcer as a model for testing of drugs for ulcerogenic side effects

Szelenyi, Istvan; Thiemer, K

doi:10.1007/bf00351774

Cited by 105 publications

(71 citation statements)

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“…The inner surface of the stomach was examined with a dissecting microscope (Nikon SMZ800) and the number of gastric lesions was counted. The ulcer index was calculated according to the method of Szelenyi & Thiemer (1978).…”

Section: Acute Gastric Mucosa Lesionsmentioning

confidence: 99%

Antiulcerogenic activity of Indigofera truxillensis Kunth

et al. 2006

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Cola-Miranda, M; Barbastefano, V.; Hiruma-Lima, C.A.; Calvo, T.R.; Vilegas, W. and Brito, A.R.M.S. Antiulcerogenic activity of Indigofera truxillensis Kunth. Biota Neotrop. Sep/Dec 2006 vol. 6, no. 3 http://www.biotaneotropica.org.br/v6n3/ pt/abstract?article+bn01006032006 ISSN 1676-0611The genus Indigofera (Fabaceae) is used in folk medicine to treat gastrointestinal pain. In this study, we investigated the antiulcerogenic properties of Indigofera truxillensis Kunth. Oral administration of MeOH extract did not produce any signals of acute toxicity. The antiulcerogenic activity was assessed in different models of acute gastric ulcers (100% ethanol, piroxicam 30 mg.kg -1 , hypothermic restraint stress and pylorus ligature) in mice and rats. The animals were treated with the drugs lanzoprazole (30 mg.kg -1 ) or cimetidine (100 mg.kg -1 ) as positive controls depending on the performed model. In another experiment with ethanol-induced ulcers in rats, N-ethylmaleimide (NEM), a sulfhydryl group blocker, was also used. The MeOH extract, at doses of 250, 500 and 1000 mg.kg -1 , inhibited the gastric lesions in all experiments: a) by 62%, 69% and 32%, respectively, in piroxicam-induced lesions, b) by 43%, 71% and 98%, in ethanol-induced lesions, c) by 69%, 64 and 89%, in hypothermic-restraint stress-induced lesions, d) by 73%, 82% and 84%, in pylorus ligature lesions. Significant changes in the total gastric acid levels were also found after intraduodenal administration of the MeOH extract in the ligated pylorus model. Pre treatment with NEM reduced partially the antiulcerogenic activity of the MeOH extract in ethanolinduced gastric lesions. This result indicates an increase in the levels of non-protein sulfhydryl groups by MeOH extract in the gastric mucosa. These results indicate that the MeOH extract has antisecretory and citoprotective effects that may be related to the presence of flavonoids detected by phytochemical analysis. O gênero Indigofera (Fabaceae) é utilizado na medicina tradicional para distúrbios gastrintestinais. Em nosso trabalho foi investigada a propriedade antiulcerogênica da Indigofera truxillensis Kunth. A administração oral do extrato metanólico (MeOH) não produziu efeitos tóxicos. A atividade antiulcerogênica foi avaliada em diferentes modelos agudos de úlcera gástrica (etanol 100%, piroxicam 30 mg.kg -1 , estresse por retenção e frio e ligadura do piloro) em camundongos e ratos. Os animais foram tratados com lansoprazol (30 mg.kg -1 ) ou cimetidina (100 mg.kg -1 ), que foram utilizados como controle positivo dependendo do modelo testado. Em outro experimento com úlcera induzida por etanol em ratos, N-etilmaleimida (NEM), um bloqueador dos compostos sulfidríla, também foi utilizado. O extrato metanólico, nas doses de 250, 500 e 1000 mg.kg -1 , inibiu significativamente as lesões gástricas em todos os experimentos: a) 62%, 69% e 32%, respectivamente, nas lesões gástricas induzidas por piroxicam, b) 43%, 71% e 98%, nas lesões gástricas induzidas por etanol, c) 69%, 64% e 89%, nas lesões ...

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Section: Acute Gastric Mucosa Lesionsmentioning

confidence: 99%

Antiulcerogenic activity of Indigofera truxillensis Kunth

et al. 2006

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“…Wistar rats (200-250 g) were subjected to an ethanolinduced ulcer assay [12]. These rats were fasted for 24 h (free access to water) before the experiment.…”

Section: Ethanol Ulcerationmentioning

confidence: 99%

“…One hour after treatment with the Tween, lanzoprazole and M. ilicifolia, 1ml of absolute ethanol was orally administered to the rats. Thirty minutes later, the animals were euthanized and their stomachs were removed to determine ulcer index [12].…”

Section: Ethanol Ulcerationmentioning

confidence: 99%

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Anti-ulcerogenic Action Mechanism of <i>Maytenus ilicifolia</i> Detected by mRNA RT-PCR and Free Radical Scavenging Activity Evaluated by ESR

Pazzini¹

2017

AJHR

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Abstract:Maytenus ilicifolia, known as "espinheira-santa", is used in the popular medicine for peptic ulcer treatment.This work evaluates the preventive and curative actions of M. ilicifolia in animals, the healing activity by EGF expression detected by RT-PCR as well as the anti-oxidative activity by Electron Spin Resonance spectroscopy (ESR). Oral administration of M. ilicifolia (300 and 500 mg/kg, p.o.) one hour before the ulcerogenic agent application prevented the ulcer formation 42 ± 0.98% and 72 ± 1.2% (p <0.05 and p <0.001, respectively), in comparison with the negative control tween 80, preserving the cytoprotection characteristics of the gastric mucosa and assuring the integrity of gastric glands and gastric fossets. The animals treated with tween, had the epithelium and the mucosa layer damaged and accentuated vascularization. The healing activity of M. ilicifolia (500mg/kg, p.o.) was 71 ± 1.4% (p <0.001) in chronic ulcer experiments induced by acetic acid. EGF expression detected by RT-PCR confirmed the healing activity. Histological analysis showed the recovery of the mucosal layer and the epithelium surface harmed by the acetic acid. Studies in vitro by Electron Spin Resonance spectroscopy assessed the anti-oxidative action of M ilicifolia. Experiments with DPPH (2.2-Diphenyl -1-picrylhydrazyl) demonstrated that the polar fraction of M. ilicifolia presents an IC 50 of 0.68±0.09 mg/ml. The value of 9±2 mg/ml was found for IC 50 in experiments with the radical OH, produced by the Fenton Reaction and detected through spin adduct DMPO-OH. Mass spectrometry analysis of crude extracts and fractions from M. ilicifolia was carried out and compounds of pentacyclic triterpenes class were identified.

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“…The ulcerative lesions index (U.L.I.) was calculated according to the methodology described by Szelenyi & Thiemer (1978).…”

Section: Ethanol/hcl-induced Gastric Lesionsmentioning

confidence: 99%

Biological activity of Herissantia crispa (L.) Brizicky

Lima¹,

Costa²,

Matias³

et al. 2009

Rev. bras. farmacogn.

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RESUMO: "Atividade biológica da Herissantia crispa (L.) Brizicky". O extrato metanólico bruto (EMeOH) das partes aéreas da Herissantia crispa (L.) Brizicky, planta rica em flavonóides e que não possui estudos farmacológicos, foi testada para avaliar sua atividade sob os parâmetros comportamentais e determinar a dose letal 50 (DL 50 ) em camundongos; atividade antimicrobiana e antiulcerogênica. O EMeOH (5000 mg/kg, v.o. ou 2000 mg/kg i.p.) não alterou os parâmetros comportamentais e não causou mortes nos animais. A amostra vegetal em estudo inibiu o crescimento bacteriano. O EMeOH (750 mg/kg) apresentou atividade anti-diarréica. O EMeOH (250, 500 e 750 mg/kg) foi capaz de inibir as lesões gástricas induzidas pelo 0,3 M HCl/etanol 60% em camundongos. Desta forma, pode-se concluir que a planta em estudo apresenta atividade antiulcerogênica, entretanto, se faz necessário avaliar tal atividade através de modelos mais específicos e estudar o mecanismo de ação pelo qual a amostra vegetal protege a mucosa gástrica.Unitermos: Herissantia crispa, Malvaecae, avaliação comportamental, atividade antibacteriana, atividade antiulcerogênica. ABSTRACT:The crude methanol extract (EMeOH) of the aerial parts of Herissantia crispa (L.) Brizicky, plant riches in flavonoids and without pharmacological studies, was tested to value its activity under the behaviour parameters and to determine the lethal dose (LD 50 ) in mice; antimicrobial and antiulcerogenic activities. The EMeOH (5,000 mg/kg, v.o. or 2,000 mg/kg i.p.) did not alter the behaviour parameters and there were not mice deaths. The extract inhibited the bacterial growth. The EMeOH (750 mg/kg) showed anti-diarroeal activity. The EMeOH (250, 500 and 750 mg/kg) decreased the gastric lesions induced by 0.3 M HCl/ethanol 60% in mice. In conclusion, the EMeOH presents anti-ulcerogenic activity;, however it is necessary to value the antiulcerogenic activity in more specific models and to study the action mechanism by which the vegetable sample protects the gastric mucosa.

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Distention ulcer as a model for testing of drugs for ulcerogenic side effects

Cited by 105 publications

References 6 publications

Antiulcerogenic activity of Indigofera truxillensis Kunth

Antiulcerogenic activity of Indigofera truxillensis Kunth

Anti-ulcerogenic Action Mechanism of <i>Maytenus ilicifolia</i> Detected by mRNA RT-PCR and Free Radical Scavenging Activity Evaluated by ESR

Biological activity of Herissantia crispa (L.) Brizicky

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Distention ulcer as a model for testing of drugs for ulcerogenic side effects

Cited by 105 publications

References 6 publications

Antiulcerogenic activity of Indigofera truxillensis Kunth

Antiulcerogenic activity of Indigofera truxillensis Kunth

Anti-ulcerogenic Action Mechanism of &lt;i&gt;Maytenus ilicifolia&lt;/i&gt; Detected by mRNA RT-PCR and Free Radical Scavenging Activity Evaluated by ESR

Biological activity of Herissantia crispa (L.) Brizicky

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Anti-ulcerogenic Action Mechanism of <i>Maytenus ilicifolia</i> Detected by mRNA RT-PCR and Free Radical Scavenging Activity Evaluated by ESR