Distance-based microfluidic quantitative detection methods for point-of-care testing

Tian, Tian; Li, Jiuxing; Song, Yanling; Zhou, Lili; Zhu, Zhi; Yang, Chaoyong

doi:10.1039/c5lc01562f

Cited by 150 publications

(75 citation statements)

References 70 publications

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“…Owing to the advantages over laboratory-based diagnostics, POC diagnostics are used for the prevention and control of infectious diseases and monitoring of health conditions in non-laboratory settings, where well-trained technicians and sophisticated laboratory infrastructure are not available [5, 6]. For example, paper-based POC diagnostics such as glucose strips or pregnancy tests can be used by end-users at home, without requiring formal training.…”

Section: Point Of Care Diagnosticsmentioning

confidence: 99%

Flexible Substrate-Based Devices for Point-of-Care Diagnostics

Wang

Chinnasamy

Lifson

et al. 2016

Trends in Biotechnology

188

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Point-of-care (POC) diagnostics play an important role in delivering healthcare, particularly for clinical management and disease surveillance in both developed and developing countries. Currently, the majority of POC diagnostics utilize paper substrates owing to their affordability, disposability, and mass production capability. Recently, flexible polymer substrates have been investigated due to their enhanced physicochemical properties, potential to be integrated into wearable devices with wireless communications for personalized health monitoring, and ability to be customized for POC diagnostics. Here, we focus on the latest advances in developing flexible substrate-based diagnostic devices, including paper and polymers, and their clinical applications at the POC.

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Section: Point Of Care Diagnosticsmentioning

confidence: 99%

Flexible Substrate-Based Devices for Point-of-Care Diagnostics

Wang

Chinnasamy

Lifson

et al. 2016

Trends in Biotechnology

188

View full text Add to dashboard Cite

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“…In this work, the non‐carcinogenic 3,3’,5,5’‐tetramethyl benzidine (TMB)‐H 2 O 2 was used as chromogenic substance for CEA detection by distance‐based measurement (Figure ). The readout was quantitative, which could be viewed by naked eye and the length of the color band was proportional to analyte concentrations . Two PADs were separately prepared for immunoassay and for distance‐based detection.…”

Section: Applications For Biomarker Detectionmentioning

confidence: 99%

Recent applications of paper‐based point‐of‐care devices for biomarker detection

Suntornsuk

2019

Electrophoresis

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Detection of biomarkers is essential for diagnosis and prognosis of diseases for healthcare teams to provide timely appropriate treatments. Reliable, inexpensive, and sensitive devices are desirable to serve this purpose. Paper‐based analytical devices (PADs) have gained enormous attention during the past decade as point‐of‐care devices for biomarker detection due to their simplicity, portability, and biocompatibility. Importantly, the devices highly comply with the WHO “ASSURED” concept (i.e. Affordable, Sensitive, Specific, User‐friendly, Rapid and Robust, Equipment free, and Deliverable to end‐users). Thus, PADs could be sustainably alternative tools for biomarker detection, especially in resources‐constraint areas where budget, skillful operators, and health infrastructure are limited. First, this review introduces overviews of biomarkers, their detection and brief history of PADs. Second, description of common fabrication and detection techniques in PADs are provided. Then, it highlights recent state of the art technologies and applications of PADs for various biomarker detection published during 2018 to May 2019. Applications for tumor marker, cardiac and coronary heath disease, and metabolic disorder biomarker detection are tabulated and selected examples are described in details. Finally, it discusses the current challenges, advances, and novel applications of PADs. The review would be valuable for healthcare workers using PADs as cost‐effective point‐of‐care devices for biomarker detection and for researchers working on future developments of the devices.

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“…The addition and mixing of reagents can be automated using chambers containing pre‐loaded reagents and designed to produce a turbulent flow while the sample flows through it. Droplet‐generating microfluidic systems can deliver fluids in a complex, automated and specific sequence …”

Section: Sample Preparationmentioning

confidence: 99%

“…Droplet-generating microfluidic systems can deliver fluids in a complex, automated and specific sequence. 13…”

Section: Chemical Techniquesmentioning

confidence: 99%

Innovative technologies for point‐of‐care testing of viral hepatitis in low‐resource and decentralized settings

Duchesne

Lacombe

2017

Journal of Viral Hepatitis

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According to the Global Burden of Diseases, chronic viral hepatitis B and C are one of the most challenging global health conditions that rank among the first causes of morbidity and mortality worldwide. Low- and middle-income countries are particularly affected by the health burden associated with HBV or HCV infection. One major gap in efficiently addressing the issue of viral hepatitis is universal screening. However, the costs and chronic lack of human resources for using traditional screening strategies based on serology and molecular biology preclude any scaling-up. Point-of-care tests have been deemed a powerful potential solution to fill the current diagnostics gap in low-resource and decentralized settings. Despite high interest resulting from their development in recent years, very few point-of-care devices have reached the market. Scaling down and automating all testing steps in 1 single device (eg, sample preparation, detection and readout) is indeed challenging. But innovations in multiple disciplines such as nanotechnologies, microfluidics, biosensors and synthetic biology have led to the creation of chip-sized laboratory systems called "lab-on-a-chip" devices. This review aims to explain how these innovations can overcome technological barriers that usually arise for each testing step while developing integrated point-of-care tests. Point-of-care test prototypes rarely meet the requirements for mass production, which also hinders their large-scale production. In addition to logistical hurdles, legal and economic constraints specific to the commercialization of in vitro diagnostics, which have also participated in the low transfer of innovative point-of-care tests to the field, are discussed.

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Distance-based microfluidic quantitative detection methods for point-of-care testing

Cited by 150 publications

References 70 publications

Flexible Substrate-Based Devices for Point-of-Care Diagnostics

Flexible Substrate-Based Devices for Point-of-Care Diagnostics

Recent applications of paper‐based point‐of‐care devices for biomarker detection

Innovative technologies for point‐of‐care testing of viral hepatitis in low‐resource and decentralized settings

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