Dissipation and gas chromatographic method for the determination of profenofos residues in/on green pea and cucumber

Self Cite

Herein, a novel, rapid, reliable, simple method validation and simultaneous quantification of 11 bioactive compounds (mostly xanthones) have been described. International Conference on Harmonization guidelines were used for the analytical method validation. Good linearity, repeatability, intra‐ and inter‐day precision, accuracy and reliability were well‐illuminated in the method validation procedure. The calibration curves showed a good linear relationship (r > 0.999) within test range. Precision was evaluated by intra‐ and inter‐day tests with relative standard deviation <2.79% and accuracy validation recovery of 74.16%–91.84%. On quantification study, the validated method described the high content of bioactive xanthone derivatives, including 1‐hydroxy‐3, 5‐dimethoxyxanthone (7), 2‐(allyloxy)‐8‐hydroxy‐1, 6‐dimethoxyxanthone (6) 1, 7, 8‐trihydroxy‐3‐methoxyxanthone (9) and Coxanthone E (5) in Codonopsis ovata, which is advantageous given the numerous pharmacological and biological effects associated with these compounds, which mostly exhibit anti‐cancerous, antioxidant, anti‐inflammatory, anti‐mutagenic and anti‐obesity effects. The bulk abundance of these compounds can also be used for further modification to produce better lead molecules for drug discovery with low toxicity and high potency. The proposed method makes it possible to simultaneously determine all bioactive compounds in one run and can be extended to marker‐based standardization of herbal formulations in medicinal and pharmaceutical industries.

Section: Introductionsupporting

confidence: 81%

Development, method validation and simultaneous quantification of eleven bioactive natural products from high‐altitude medicinal plant by high‐performance liquid chromatography

Dar

Raina

Kumar

2022

Self Cite

“…The average per capita daily consumption of vegetables in India is 200 g (Anonymous, 2012), of which the proportion of green chilies in the daily diet is a maximum of 5% (10 g day −1 ). Therefore, the TMRC has been calculated by multiplying the residue level with the daily consumption of chili (Jan et al, 2018, 2022; Wani et al, 2022; Wani, Dar, et al, 2019; Wani, Jan, et al, 2019). During the experimental period, the TMRC values were found to be 0.01212 and 0.01207 mg person −1 day −1 and 0.01953 and 0.01948 mg person −1 day −1 for maximum and average spiromesifen residues at the recommended dose (96 g. a.i.…”

Section: Resultsmentioning

confidence: 99%

Dissipation kinetics, risk assessment, and waiting period of spiromesifen on chili fruits using gas chromatography–electron capture detector

Dudwal

Pathan

Jakhar

et al. 2023

Self Cite

A supervised field trial was designed in Rajasthan Agricultural Research Institute, Durgapura, Jaipur, Rajasthan, to assess the dissipation and persistence of spiromesifen in chili fruits. Spiromesifen (22.9% suspension concentrate) was sprayed two times at an interval of 10 days at the recommended dose (96 g. a.i. ha−1) and double the recommended dose (192 g. a.i. ha−1) with four replications. Sampling was done according to the planned interval of days after the second spray. Extraction and cleanup were performed using the modified QuEChERS (quick, easy, cheap, effective, rugged, and safe) method and the spiromesifen residue was analyzed by GC–electron capture detector and confirmation performed using GC–MS. The average initial deposit of spiromesifen was 1.207 mg kg−1and 1.948 mg kg−1at the recommended and double the recommended dose, respectively. The half‐life values of spiromesifen ranged between 2.7 and 3.2 days at the recommended and double the recommended dose. The safe waiting period was calculated for the respective doses and it was concluded that an average of 7 days is safe for picking. The FSSAI (Food Safety and Standards Authority of India) have set the maximum residue limit of 0.1 mg kg−1 for spiromesifen in green chili. The theoretical maximum residue contribution value of spiromesifen was lower than the maximum permissible intake at both the applications on the 0th day. Hence, there will be no adverse effects on human health after consumption of green chilies.

“…An intensive literature review revealed that numerous methods, like UV spectrophotometry, gas chromatography–mass spectrometry (GC–MS), high‐performance liquid chromatography (HPLC) and high performance thin liquid chromatography, can be employed for the quantification of cypermethrin from natural sources, but such methods are either tedious or lack sensitivity and specificity and are expensive. However, GC appears to be the method of choice, affording the separation and quantification of insecticides (Wani et al, 2022). Its simplicity, ecofriendly and cost‐effectiveness are the advantages of using GC instead of other techniques for the quantification of pesticides from natural sources.…”

Section: Introductionmentioning

confidence: 99%

Risk assessment, development and validation of a GC‐ECD‐based method for the quantification of cypermethrin from green pea

Jan

Dar

Wani

et al. 2022

Self Cite

The present work describes the persistence, dissipation behaviour, half‐life, risk assessment and novel gas chromatography method for the residue estimation of cypermethrin in green pea by spraying cypermethrin 10EC at 50 g a.i. ha−1 at fruiting stage followed by another application at a 10 day interval. The sample extraction and cleanup was followed bya modified quick, easy, cheap, effective, rugged, and safe method, and the residues of cypermethrin were determined using a validated gas chromatography method. The initial deposits were found to be 1.21 mg kg−1 following the application of insecticide at 50 g a.i. ha−1. Cypermethrin residues declined to below the detection limit of 0.05 mg kg−1 after 15 days at the recommended dosage. The half‐life of cypermethrin was 2.66 days at 50 g a.i. ha−1. For risk assessment studies, the waiting period of 15 days is recommended as safe for consumption for the insecticide. The GC‐ECD method was validated according to the SANTE guidelines by various analytical parameters including linearity, accuracy, detection and quantification limits. The developed method is simple, selective and repeatable, and can be used for the standardization of pesticides on fruits and vegetables.