Disrupted spermatogenesis in a metabolic syndrome model: the role of vitamin A metabolism in the gut–testis axis

Zhang, Teng; Sun, Peng; Geng, Qi Rong; Fan, Haitao; Gong, Yutian; Hu, Yuxin; Shan, Li-Ying; Sun, Yuanchao; Shen, Wei; Zhou, Yang

doi:10.1136/gutjnl-2020-323347

Cited by 99 publications

(50 citation statements)

References 37 publications

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“…Indeed, the existence of a gut-gonad axis has been demonstrated in Drosophila, wherein the male intestine secretes citrate to the adjacent testes and promotes sperm maturation (Hudry et al, 2019). A similar gutgonad axis was recently described in a sheep model of diet-induced metabolic syndromes (Zhang et al, 2021). In both these models, metabolic perturbations altered spermatogenesis and sperm numbers.…”

Section: Discussionmentioning

confidence: 88%

Nutritional dependence of sperm mitochondrial metabolism and small RNA biogenesis

Ramesh

Skog

Nätt

et al. 2021

Preprint

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A wide spectrum of exogenous factors, including diet, environmental pollutants, stress, and seasonal changes have major impact on sperm quality and function. The molecular basis, however, that explains this susceptibility remains largely unknown. Using a combination of proteomics and small RNA (sRNA) sequencing, we show that Drosophila sperm display rapid molecular changes in response to dietary sugar, both in terms of metabolic/redox proteins and sRNA content, particularly miRNA and mitochondria derived sRNA (mt-sRNA). Thus, results from two independent omics point at the dynamics of mitochondria as the central aspect in rapid metabolic adjustments in sperm. Using specific stains and in vivo redox reporter flies, we show that diet indeed rapidly alters the production of mitochondrial derived reactive oxygen species (ROS). Quenching ROS via supplementation of N acetyl cysteine reduces diet-upregulated miRNA, but not mitochondrial-sRNA. Together, these results open new territories in our search for the mechanistic understanding of sperm health and disease.

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Section: Discussionmentioning

confidence: 88%

Nutritional dependence of sperm mitochondrial metabolism and small RNA biogenesis

Ramesh

Skog

Nätt

et al. 2021

Preprint

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“…Additionally, Akkermansiaceae, especially Akkermansia muciniphila, plays a protective role in diet induced obesity and other diseases [38]. What's more, the relative abundance of speci c genus Ruminococcaceae_NK4A214_group was reduced in HF group, and it was signi cantly correlated with bile acid and Vitamin A levels, which plays a vital role in the treatment of rats with ulcerative colitis [39]. Christensenellaceae_R-7_group as a probiotic is signi cantly negatively correlated with metabolic diseases such as BMI and in ammation, such as IBD and metabolic syndrome [40].…”

Section: Discussionmentioning

confidence: 99%

Microbiome-Metabolome Analyze The Immune Microenvironment of Cecal Contents, Soft and Hard Feces of Hyplus Rabbits

et al. 2021

Preprint

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Background: Intestinal microbiota and its metabolites play a vital role in host growth, development and immune regulation. Methods: This study analyzed the microbial community distribution, cytokines and short chain fatty acids (SCFAs) content of cecal contents (Con) group, soft feces (SF) group and hard feces (HF) group of 140-day-old Hyplus rabbits, and verified the effect of soft feces on cecal immune microenvironment by fasting soft feces.Results: The results showed that there were significant differences in the levels of phylum and genus composition, cytokines and SCFAs among Con group, SF group and HF group. In addition, metabolic pathway enrichment analysis found that there were two significantly up-regulated differential metabolic pathways in SF group and HF group compared with con group, P125-PWY, namely the super pathway of (R, R) - butanediol biosynthesis and P341-PWY, namely the glycolysis V pathway. At the same time, Christensenellaceae_R-7_Group and Lachnoclostridium are significantly enriched in the above two pathways. The correlation analysis of cytokines and SCFAs with differential microbial communities showed that Muribaculaceae and Ruminococcaceae_UCG-014, Ruminococcaceae_NK4A214_group and Christensenellaceae_R-7_Group are closely related to cytokines and SCFAs. After fasting soft feces (CP), the contents of SCFAs and cytokines (IL-4, IL-10) in cecum decreased significantly, while cytokines (TNF-a, IL-1β) increased significantly. The results of multiple immunofluorescence showed that the expression of claudin-1, occludin and ZO-1 related to intestinal immune barrier increased significantly in CP Group. Conclusions: In conclusion, soft feces are not only rich in probiotics and SCFAs, but also play a very important role in improving the immune microenvironment of cecum. This study may provide a valuable reference for the treatment of inflammatory intestinal diseases.

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“…Moreover, it has been suggested that the commensal microbiome may influence the signaling mechanisms of vitamin K and vitamin A, altering biochemical components of the basement membrane and causing infertility in men 73 . It is reasonable to presume that gut microbes and testicular microbes together influence human reproductive function, in conjunction with Zhang et al.’s earlier observation that altered gut microbes affect vitamin A metabolism in a model of metabolic syndrome and ultimately impact sperm development 74 …”

Section: Testicular Microbiotamentioning

confidence: 99%

Exploring the role of gut microbiome in male reproduction

Wang

Zuo-gang

2022

Andrology

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Background The impact of the gut microbiome on the organism has become a growing research focus with the development of 16S rRNA sequencing. However, the effect of the gut microbiome in male reproduction has yet to be investigated. Objective To overview on possible mechanisms by which the gut microbiome could affect male reproduction and therapeutic opportunities related to the gut microbiome Methods Authors searched PubMed/MEDLINE, EMBASE, Web of Science, and Cochrane Library for medical subject headings terms and free text words referred to “male infertility” “testis” “gut microbiome” “insulin resistance” “erectile dysfunction” “therapy” “sex hormones” and “genital diseases” until December 2, 2021. Results Evidence suggests that immune system activation caused by the gut microbiome translocation not only leads to testicular and epididymal inflammation but can also induce insulin resistance together with gastrointestinal hormones such as leptin and ghrelin, which in turn affects the secretion of various sex hormones such as LH, FSH, and T to regulate spermatogenesis. In addition, the gut microbiome can influence spermatogenesis by controlling and metabolizing androgens as well as affecting the blood–testis barrier. It also promotes vascular inflammation by raising trimethylamine‐N‐oxide (TMAO) levels in the blood, which causes erectile dysfunction. The testicular microbiome and gut microbiome can interact to influence male reproductive function. This study discusses therapeutic options such as probiotics, prebiotics, and fecal microbiota transplantation, as well as the challenges and opportunities behind ongoing research, and emphasizes the need for additional research in the future to demonstrate the links and underlying mechanisms between the gut microbiome and male reproduction. Therapeutic options such as probiotics, prebiotics, and fecal microbiota transplantation are potential treatments for male infertility. Discussion and conclusion Gut microbiota may have a causal role in male reproduction health, therapeutic strategies such as supplementation with appropriate probiotics could be undertaken as a complementary treatment. In the future, additional research is needed to demonstrate the links and underlying mechanisms between the gut microbiome and male reproduction.

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Disrupted spermatogenesis in a metabolic syndrome model: the role of vitamin A metabolism in the gut–testis axis

Cited by 99 publications

References 37 publications

Nutritional dependence of sperm mitochondrial metabolism and small RNA biogenesis

Nutritional dependence of sperm mitochondrial metabolism and small RNA biogenesis

Microbiome-Metabolome Analyze The Immune Microenvironment of Cecal Contents, Soft and Hard Feces of Hyplus Rabbits

Exploring the role of gut microbiome in male reproduction

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