Disposable Reagentless Electrochemical Immunosensor Array Based on a Biopolymer/Sol-Gel Membrane for Simultaneous Measurement of Several Tumor Markers

Wu, Jie; Yan, Feng; Zhang, Xiaoqing; Yan, Yuetian; Tang, Jinhai; Ju, Huangxian

doi:10.1373/clinchem.2007.102350

Cited by 78 publications

(49 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The biosensor exhibited a fast amperometric response (5 s), a good linear response over a wide range of concentrations from 5.0×10 −6 to 1.4×10 −3 M, and a low detection limit of 4.01×10 −7 M with good stability and sensitivity [61]. Furthermore, by immobilization of four different horseradish peroxidase-labeled antibodies modified GNPs on screen-printed carbon electrodes with chitosan/ sol-gel, Ju's group fabricated a disposable reagentless electrochemical immunosensor array, which implemented simultaneous multianalyte testing [75].…”

Section: Gnps Act As Electron Transfer "Electron Wires"mentioning

confidence: 99%

Gold nanoparticle-based biosensors

2010

View full text Add to dashboard Cite

The unique properties of gold nanoparticles have stimulated the increasing interest in the application of GNPs in interfacing biological recognition events with signal transduction and in designing biosensing devices exhibiting novel functions. The optical properties of GNPs provide wide range opportunities for construction optical biosensors. The excellent biocompatibility, conductivity, catalytic properties and high surface-to-volume ratio and high density of GNPs facilitate extensive application of GNPs in construction of electrochemical and piezoelectric biosensors with enhanced analytical performance with respect to other biosensor designs. In this article, the recent advances in construction of GNP-based optical, electrochemical and piezoelectric biosensors are reviewed, and some illustrative examples given, with a focus on the roles GNPs play in the biosensing process and the mechanism of GNPs for improving the analytical performances. Finally, the review concludes with an outline of present and future research for the real-world applications.

show abstract

Section: Gnps Act As Electron Transfer "Electron Wires"mentioning

confidence: 99%

Gold nanoparticle-based biosensors

2010

View full text Add to dashboard Cite

show abstract

“…However, crosstalk can potentially occur due to the diffusion of electroactive product generated at one electrode to a neighboring electrode to produce an interfering amperometric response. Its elimination mainly focuses on making the distance between adjacent electrodes larger than the diffusion distance of electroactive substances [25][26][27][28] or immobilizing electron transfer mediators or electroactive enzyme on sensor surfaces to perform reagentless detection [8,9,29,30].…”

Section: Introductionmentioning

confidence: 99%

“…1). With a sandwich format for immunoassay and capture of AuNP labeled antibodies on the disposable chip, the developed multiplexed immunoassay method avoided the crosstalk and a deoxygenation process that is generally essential in amperometric immunoassays [8][9][10][11]29,30], thus simplifying greatly the detection process. This method could immediately be extended to multianalyte determination for the need of clinical diagnostics.…”

Section: Introductionmentioning

confidence: 99%

Gold nanoparticle as an electrochemical label for inherently crosstalk-free multiplexed immunoassay on a disposable chip

Leng

Lai

Yan

et al. 2010

Analytica Chimica Acta

Self Cite

View full text Add to dashboard Cite

“…The total analysis time of 5 min allowed clinically relevant concentrations of carcinoma, carbohydrate, and carcinoembryonic antigens to be determined. Previous work in this area, leading to the four-element array, has been published [54,55] and a following paper with the same four-element array, including improved validation results, is also available [56].…”

Section: Array Elements Modified With Antibodies or Antigensmentioning

confidence: 99%

Electrochemical Arrays for Bioassay Applications

Cortón

Mikkelsen

2016

Trends in Bioelectroanalysis

View full text Add to dashboard Cite

This chapter presents a survey of developments and bioassay applications of individually addressable electrochemical arrays covering the past two decades and including over 90 references. Many different array designs have been fabricated, from macroscopic devices that have two to four sensing elements to ultramicroelectrode arrays containing as many as 12,000 individually addressable micrometer-sized working electrodes. Some inexpensively fabricated arrays are intended for single sample analysis and are disposable, while others are encapsulated for incorporation into reusable microfluidic systems. Devices and assays are grouped according to the types of modifications done to the sensing elements, and sections are included to consider unmodified elements as well as modifications with enzymes, antibodies or antigens, nucleic acids, in addition to viable cells and tissues. Some trends have been identified, and possible future directions are suggested.

show abstract

Disposable Reagentless Electrochemical Immunosensor Array Based on a Biopolymer/Sol-Gel Membrane for Simultaneous Measurement of Several Tumor Markers

Abstract: BACKGROUND:A reagentless sensor array for simultaneous multianalyte testing (SMAT) may enable accurate diagnosis and be applicable for point-of-care testing. We developed a disposable reagentless immunosensor array for simple immunoassay of panels of tumor markers.

Cited by 78 publications

References 38 publications

Gold nanoparticle-based biosensors

Gold nanoparticle-based biosensors

Gold nanoparticle as an electrochemical label for inherently crosstalk-free multiplexed immunoassay on a disposable chip

Electrochemical Arrays for Bioassay Applications

Contact Info

Product

Resources

About