Gold nanoparticle as an electrochemical label for inherently crosstalk-free multiplexed immunoassay on a disposable chip

Leng, Chuan; Lai, Guosong; Yan, Feng; Ju, Huangxian

doi:10.1016/j.aca.2010.03.060

Cited by 80 publications

(53 citation statements)

References 34 publications

(45 reference statements)

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“…Figure 4b shows 96-well UV plates in which the antigen had been gradually and equally added to mAb and pAb solutions. For the pAb experiment, the resulting colour change was observable by the naked eye whereas no colour change was observed for the other Ab [34][35][36][37].…”

Section: The Pab-based Immunonanosensormentioning

confidence: 76%

“…As depicted in Figure 5b, the ligand caused the AuNPs to aggregate, which led to interaction between their individual SPR bands and consequently, to plasmonic coupling. As a result, unlike the reddish mFcL/AuNPs, the mFcL/AuNPs containing the bipodal ligand were purple, exhibiting a 10-nm redshift in their UV-Vis spectrum relative to that of the former (Figure 5c) [34][35][36][37]. Moreover, when 1 mL of a conc.…”

Section: Provoking Nanoparticle Aggregation To Induce An Increase In mentioning

confidence: 87%

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Control of Electron‐transfer in Immunonanosensors by Using Polyclonal and Monoclonal Antibodies

et al. 2016

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The design and operation of biosensors is not trivial. For instance, variation in the output signal during monitoring of analytes can not usually be controlled. Hence, if such control were possible, and could be triggered on demand, it would greatly facilitate system design and operation. Herein, we report the design of two types of voltamperometric immunosensors, in which the magnitude of the current output signal (differential pulse voltammetry [DPV]) can be increased or decreased as needed. The designed systems use monoclonal and polyclonal anti‐human IgG antibodies, conjugated to monopodal ferrocene‐modified gold nanoparticles that are casted onto screen‐printed carbon electrodes (Ab/mFcL/AuNPs/SPCEs). Upon addition of human IgG as antigen, the systems exhibit opposite responses according to the Ab: the current decreases when monoclonal Ab is used, whereas it increases when polyclonal Ab is used. We attributed the former response to inhibition of electron‐transfer (due to the formation of a protein layer), and the latter response, to a global increase in electron transfer (induced by the aggregation of gold nanoparticles). These effects were confirmed by studying a custom‐made lipoic acid‐based bipodal ligand, which confirmed that the increase in current is effectively induced by the aggregation of the modified nanoparticles (pAb/mFcL/AuNPs). Both sensors have large dynamic ranges, although the pAb‐based one was found to be 3.3‐times more sensitive. Tests of selectivity and specificity for ovalbumin, α‐lactalbumin and serum bovine albumin showed that the immunosensors are highly selective and specific, even in the presence of up to 1000‐fold levels of potentially competitive proteins. The limit of detection for human IgG using the pAb/mFcL/AuNP bioconjugate was estimated to be 0.85 ng/mL. The pAb/mFcL/AuNPs‐based biosensor has used to determine amounts of human IgG in real sample.

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Section: The Pab-based Immunonanosensormentioning

confidence: 76%

Section: Provoking Nanoparticle Aggregation To Induce An Increase In mentioning

confidence: 87%

Control of Electron‐transfer in Immunonanosensors by Using Polyclonal and Monoclonal Antibodies

et al. 2016

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“…AuNPs have been utilized as signal probes for electrochemical immunoassays. For example, Ju's group [62] proposed a simple, sensitive and low-cost inherently crosstalk-free multiplexed immunoassay by combining a disposable chip with AuNP as an electrochemical label. The anaytes could be detected by electrooxidization of AuNPs in 0.1 M HCl.…”

Section: Nanomaterials For Immunosensing Of Proteinsmentioning

confidence: 99%

Sensitive biosensing strategy based on functional nanomaterials

2011

Sci. China Chem.

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The first decade of the 21st century has been labeled as "the sensing decade". The functional nanomaterials offer excellent platforms for fabrication of sensitive biosensing devices, including optical and electronic biosensors. A lot of works have focused on the biofunctionalization of different nanomaterials, such as metal nanoparticles, semiconductor nanoparticles and carbon nanostructures, by physical adsorption, electrostatic binding, specific recognition or covalent coupling. These biofunctionalized nanomaterials can be used as catalysts, electronic conductors, optical emitters, carriers or tracers to obtain the amplified detection signal and the stabilized recognition probes or biosensing interface. The designed signal amplification strategies have greatly promoted the development of stable, specific, selective and sensitive biosensors in different fields. This review introduces some novel principles and detection strategies in the area of biosensing, based on functional nanomaterials. The general methods for biofunctionalization of nanomaterials with biomolecules and their biosensing application in immunoassay of protein, DNA detection, carbohydrate analysis and cytosensing are also described.

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“…Furthermore, these nanoparticles can induce the deposition Ag NPs for further amplifying the electrochemical stripping signals. Ju' group [23] reported a simple, sensitive and low-cost multiplexed immunoassay by combining a disposable chip with Au NP as an electrochemical label (Fig. 5).…”

Section: Electrochemical Stripping Analysis Of Nano Labels For Signalmentioning

confidence: 99%

Signal Amplification for Highly Sensitive Immunosensing

2017

J. Anal. Test.

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To dissolve the bottleneck problem of life and biomedical science in detection of biomolecules with low abundance and acquisition of ultraweak biological signals, highly sensitive analytical methods coupling with the specificity of biological recognition events have been quickly developed by the exploring of signal amplification strategies. These strategies have extensively been introduced into the development of highly sensitive immunosensing methods by combining with highly specific immunological recognition. They can be divided into two groups. One group of strategies attempts to transfer the immunological recognition event into large number of reporter probes or signal probes for signal readout by employing nano/micro-materials as vehicles for multi-labeling and/or molecular biological amplification for increasing the abundance of the signal molecules. The other uses nanomaterials or enzyme mimics as catalytic tools/tags to obtain enhanced detection signal. This review focuses on the significant advances in design of signal amplification strategies for highly sensitive immunosensing.

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Gold nanoparticle as an electrochemical label for inherently crosstalk-free multiplexed immunoassay on a disposable chip

Cited by 80 publications

References 34 publications

Control of Electron‐transfer in Immunonanosensors by Using Polyclonal and Monoclonal Antibodies

Control of Electron‐transfer in Immunonanosensors by Using Polyclonal and Monoclonal Antibodies

Sensitive biosensing strategy based on functional nanomaterials

Signal Amplification for Highly Sensitive Immunosensing

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