Signal Amplification for Highly Sensitive Immunosensing

Ju, Huangxian

doi:10.1007/s41664-017-0008-6

Cited by 31 publications

(12 citation statements)

References 88 publications

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“…Finally, the high surface‐to‐volume ratio of nano/microparticles also makes them attractive for detection purposes. Nano/microparticles can be used as carriers of a large number of reporter molecules, such as fluorophores, colorimetric probes, enzymes, among others, contributing to signal amplification methods [273]. For example, a microfluidic electrochemical immunoassay that uses paramagnetic nanoparticles, each conjugated with ∼90 000 antibodies and ∼200 000 horseradish peroxidase (HRP) labels has been reported to detect the presence of PSA and IL‐6 from serum samples.…”

Section: Protein Biomarkersmentioning

confidence: 99%

Microfluidic systems for the analysis of blood‐derived molecular biomarkers

Chavez-Pineda¹,

Rodriguez‐Moncayo²,

Cedillo-Alcantar³

et al. 2022

Electrophoresis

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Biomarkers are relevant indicators of the physiological state of an individual.Although biomarkers can be found in diseased tissue and different biofluids, sampling from blood plasma is relatively easy and less invasive. Among the molecular biomarkers that can be found circulating in plasma are proteins, metabolites, nucleic acids, and exosomes. Some of these plasma-circulating biomarkers are now employed for patient stratification in a broad range of diseases with high sensitivity and specificity and are useful in early diagnosis, initial risk assessment, and therapy selection. However, there is a pressing need to develop novel approaches for biomarker analysis that can be translated into clinical or other settings without complex methodologies or instrumentation. Microfluidics has been touted as a promising technology to carry out this task because it offers high-throughput, automation, multiplexed detection, and portability, possibly overcoming the bottleneck that prevent the translation of novel biomarkers to the point-of-care (POC). Here, we provide a review of the microfluidic systems that have been engineered to detect circulating molecular biomarkers in blood plasma. We also review the different microfluidic approaches for plasma enrichment, which are now being integrated with microfluidic-based biomarker analyzers. Such integration should lead to

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Section: Protein Biomarkersmentioning

confidence: 99%

Microfluidic systems for the analysis of blood‐derived molecular biomarkers

Chavez-Pineda¹,

Rodriguez‐Moncayo²,

Cedillo-Alcantar³

et al. 2022

Electrophoresis

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“…Although electrochemical biosensors are already highly sensitive, their sensitivity must be further improved for the detection of certain molecules at low concentrations or molecules that are difficult to isolate from biological samples [ 19 , 20 , 21 , 22 ]. In recent years, detection methods for specific interactions between biological recognition elements, such as antibodies, nucleotides, enzymes, and target analytes have been proposed and developed to improve the sensitivity and selectivity of detection systems [ 23 ].…”

Section: Electrochemical Biosensorsmentioning

confidence: 99%

Electrochemical Signal Amplification Strategies and Their Use in Olfactory and Taste Evaluation

Wang

Liu

et al. 2022

Biosensors

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Biosensors are powerful analytical tools used to identify and detect target molecules. Electrochemical biosensors, which combine biosensing with electrochemical analysis techniques, are efficient analytical instruments that translate concentration signals into electrical signals, enabling the quantitative and qualitative analysis of target molecules. Electrochemical biosensors have been widely used in various fields of detection and analysis due to their high sensitivity, superior selectivity, quick reaction time, and inexpensive cost. However, the signal changes caused by interactions between a biological probe and a target molecule are very weak and difficult to capture directly by using detection instruments. Therefore, various signal amplification strategies have been proposed and developed to increase the accuracy and sensitivity of detection systems. This review serves as a reference for biosensor and detector research, as it introduces the research progress of electrochemical signal amplification strategies in olfactory and taste evaluation. It also discusses the latest signal amplification strategies currently being employed in electrochemical biosensors for nanomaterial development, enzyme labeling, and nucleic acid amplification techniques, and highlights the most recent work in using cell tissues as biosensitive elements.

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“…Most importantly, they tend to become more useful and beneficial with their readiness to combine and improve detection platforms such as mass spectra, surface plasmon resonance, electrochemical, Raman spectra, fluorescent, and many more [72] as shown in Table 3. Ju [73] in his review expatiated the use of Fe 3 O 4 nanoparticles-based hybridized materials to enhance peroxidase-like activity, and thus enabling the detection of bio-materials.…”

Section: Functionalized Magnetic Nanoparticlesmentioning

confidence: 99%

Functionalized Inorganic Nanoparticles for the Detection of Food and Waterborne Bacterial Pathogens

Daramola¹,

Torimiro²,

Fadare³

et al. 2020

NNR

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Infections acquired from ingesting contaminated food and water poses an adverse effect on public health and safety, thus affecting nations' economy. Technical approaches developed over years have contributed adequately to microbial detection in food and water, yet, unveiling spaces for more improvement on early and rapid detection of pathogens. This review highlights different strategy assessing bio-functionalized inorganic nanoparticles towards the detection of pathogens in food and water samples. Conjugates of several bio-receptors and inorganic nanoparticles showed rapid, real-time, repeatability, and appreciable limit of detection in targeted pathogens. A patent referenced in this study established the biocompatibility of bio-functionalized inorganic nanoparticles mechanism. Unique attributes exhibited by bio-functionalized inorganic nanoparticles showed potential and improvement of the existing bio-sensing pathogen detection methods. Each of the identified strategies described showed a promising pathway accommodating the development of simple, and even the fabrication of low-cost materials for easy detection of bacterial pathogens in food and water products.

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Signal Amplification for Highly Sensitive Immunosensing

Cited by 31 publications

References 88 publications

Microfluidic systems for the analysis of blood‐derived molecular biomarkers

Microfluidic systems for the analysis of blood‐derived molecular biomarkers

Electrochemical Signal Amplification Strategies and Their Use in Olfactory and Taste Evaluation

Functionalized Inorganic Nanoparticles for the Detection of Food and Waterborne Bacterial Pathogens

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