Disposable immunosensors for C-reactive protein based on carbon nanotubes field effect transistors

Justino, Celine I.L.; Freitas, Ana C.; Amaral, Joana D.; Rocha-Santos, Teresa; Cardoso, Susana; Duarte, Armando C.

doi:10.1016/j.talanta.2013.03.007

Cited by 44 publications

(45 citation statements)

References 37 publications

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“…The sensitivity of the device for the same CRP concentration is much higher than various other material based FET devices. 20,21 A comparatively $10 fold higher sensitivity obtained with the PAMAM based device with respect to the recently reported 22 bare SWNT-FET for the same concentration of CRP signifies the role using PAMAM for effective biomolecular immobilization of anti-CRP with enhanced loading and a better probe orientation due to its 3-dimensional polymeric structure on the SWNT device.…”

mentioning

confidence: 66%

“…Multiple control experiments revealed that the anti-CRP immobilized on PAMAM retains its highly specific binding characteristics towards CRP analyte when attached to the SWNT network channel of the FET device. The high sensitivity of the PAMAM modified SWNT-FET device towards CRP than the previously reported 22 sensor based on the bare SWNT device signifies the suitability of PAMAM for biomolecular immobilization with high loading and better probe orientation. These results of high antigen-antibody specificity with high sensitivity over the physiological range of CRP concentration and ease of use with a small sample volume make it superior to traditional immunoassay, suggesting that this device concept may be used as a portable tool to help diagnose disease after a further suitable optimization with real clinical (blood/human serum/plasma) samples.…”

mentioning

confidence: 73%

“…Therefore, the prime novelty of the manuscript is to highlight the significance of using G-4.5 PAMAM for the site specific biomolecular immobilization of protein antibody, anti-CRP, which provides a favorable microenvironment for the immunoreaction for antigen-antibody conjugation with a high output current response than earlier reported FET devices for CRP detection. [20][21][22] This electronic immunosensor has additional advantages of being compatible with a low-cost and scalable fabrication procedure for the label-free detection of CRP biomarker.…”

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confidence: 99%

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High performance dendrimer functionalized single-walled carbon nanotubes field effect transistor biosensor for protein detection

Rajesh

Sharma

Puri

et al. 2016

Applied Physics Letters

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We report a single-walled carbon nanotube (SWNT) field-effect transistor (FET) functionalized with Polyamidoamine (PAMAM) dendrimer with 128 carboxyl groups as anchors for site specific biomolecular immobilization of protein antibody for C-reactive protein (CRP) detection. The FET device was characterized by scanning electron microscopy and current-gate voltage (I-Vg) characteristic studies. A concentration-dependent decrease in the source-drain current was observed in the regime of clinical significance, with a detection limit of ∼85 pM and a high sensitivity of 20% change in current (ΔI/I) per decade CRP concentration, showing SWNT being locally gated by the binding of CRP to antibody (anti-CRP) on the FET device. The low value of the dissociation constant (Kd = 0.31 ± 0.13 μg ml−1) indicated a high affinity of the device towards CRP analyte arising due to high anti-CRP loading with a better probe orientation on the 3-dimensional PAMAM structure.

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confidence: 66%

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confidence: 73%

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confidence: 99%

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High performance dendrimer functionalized single-walled carbon nanotubes field effect transistor biosensor for protein detection

Rajesh

Sharma

Puri

et al. 2016

Applied Physics Letters

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“…Detection of tumor biomarkers for breast cancer [52,53], hepatic carcinoma [54], oral squamous cell carcinoma [55], pancreatic cancer [56], and bladder cancer [57] have also been reported. Moreover, other disease biomarkers related to cardiovascular diseases [58][59][60], thyroid hormone [61], venous thromboembolism [62], Alzheimer [63], and diabetes [64,65] have been detected with very low detection limits. Table 2 evidences the ultrasensitive detection of pathogenic microorganisms associated with human health and food safety, such as Salmonella [66,67] and Escherichia coli [68][69][70][71] bacteria, rotavirus [72] and bacteriophage [69] viruses, and parasitic protozoan [73].…”

Section: Trends In Fet-based Immunosensors Using Nanomaterials As Senmentioning

confidence: 99%

“…Their physical properties are strongly dependent on the way that graphitic sheets are wrapped to form the tubes (chirality), causing them to exhibit metallic or semiconducting characteristics [99]. In particular, SWCNTs have been explored as promising building blocks in the construction of FET-based immunosensors with improved sensibility [58,69,76,[80][81][82]103]. SWCNTs display high electron transfer because all carbon atoms are …”

Section: Carbon Nanotubesmentioning

confidence: 99%

Recent Trends in Field-Effect Transistors-Based Immunosensors

Moraes

Kubota

2016

Chemosensors

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Abstract:Immunosensors are analytical platforms that detect specific antigen-antibody interactions and play an important role in a wide range of applications in biomedical clinical diagnosis, food safety, and monitoring contaminants in the environment. Field-effect transistors (FET) immunosensors have been developed as promising alternatives to conventional immunoassays, which require complicated processes and long-time data acquisition. The electrical signal of FET-based immunosensors is generated as a result of the antigen-antibody conjugation. FET biosensors present real-time and rapid response, require small sample volume, and exhibit higher sensitivity and selectivity. This review brings an overview on the recent literature of FET-based immunosensors, highlighting a diversity of nanomaterials modified with specific receptors as immunosensing platforms for the ultrasensitive detection of various biomolecules.

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Carbon Nanomaterials Applied for the Treatment of Inflammatory Diseases: Preclinical Evidence

Soltani

Guo

Bianco

et al. 2020

Advanced Therapeutics

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In the last decade, graphene‐based nanomaterials and carbon dots have joined the family of carbon materials mainly composed of graphite, diamond, fullerene, and carbon nanotubes. Carbon nanomaterials have been widely exploited in various fields from electronics and materials science to nanomedicine. The studies on their effect on the immune system have revealed that they possess intrinsic anti‐inflammatory properties, reducing the production of proinflammatory cytokines and modulating immune cell maturation. In addition, their large specific surface area associated with high biocompatibility allows their use as carriers for the delivery of anti‐inflammatory agents. They can also contribute to the diagnosis of inflammatory diseases as biosensors for low‐limit detection and quantification of inflammation‐related biomarkers in body fluids and tissues allowing to monitor the concentration of drugs in urine and guide personalized drug usage. Finally, they are used as adsorbents for blood plasma purification in the clinical treatment of sepsis through efficient removal of certain cytokines. This review focuses on the intrinsic anti‐inflammatory properties of carbon nanomaterials. An overview is provided on their use as carriers of anti‐inflammatory drugs, as biosensors, and for blood purification in the context of inflammatory diseases. The potential of carbon nanomaterials for clinical translation is also critically discussed.

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Disposable immunosensors for C-reactive protein based on carbon nanotubes field effect transistors

Cited by 44 publications

References 37 publications

High performance dendrimer functionalized single-walled carbon nanotubes field effect transistor biosensor for protein detection

High performance dendrimer functionalized single-walled carbon nanotubes field effect transistor biosensor for protein detection

Recent Trends in Field-Effect Transistors-Based Immunosensors

Carbon Nanomaterials Applied for the Treatment of Inflammatory Diseases: Preclinical Evidence

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