Discrimination Between the Immature Stages of Cermatitis capitata and Anastrepha fraterculus (Diopters: Tephritidae) Populations by Random Amplified polymorphic DNA Polymerase Chain Reaction

Abstract

A random amplified polymorphic DNA polymerase chain reaction method using 10-base long commercial random primers was used to identify polymorphisms in Argentine populations of 2 fruit fly pests. A fast and reliable discrimination between Mediterranean fruit fly, Ceratitis capitata (Wiedemann), and Anastrepha fraterculus (Wiedemann) immature or mature stages was obtained using as few as 1 insect per assay. The population of origin of immature individuals can be identified unambiguously using 2 primers.

Genetic Relationships Among <I>Aedes aegypti</I> (Diptera: Culicidae) Populations from Argentina Using Random Amplified Polymorphic DNA Polymerase Chain Reaction Markers

“…Molecular markers generated by RAPD-PCR were useful in determining the geographic origins of a weevil (Williams et al 1994), stored product moth (Dowdy and McGaughey 1996), gypsy moth (Schreiber et al 1997), and fruit flies (Reyes and Onchado 1998), and in estimating gene flow and genetic variability in C. hominivorax (InfanteMalachias et al 1999;Azeredo-Espin and Lessinger 2006) as well as several species of medically-important mosquitoes (de Sousa et al 2001;Gonzáles et al 2007;Hiragi et al 2009). Additionally, inter-and intraspecific differentiation by RAPD-PCR has been applied to the Mediterranean fruit fly (Sonvico et al 1996), triatomine bugs (Garcia et al 1998), and horn flies (Castiglioni et al 2005), with the capability of identifying biotypes or ecotypes/ecoraces of various insect species (Guirao et al 1997;Pornkulwat et al 1998;Saha and Kundu 2006). Here, our objective was to further develop RAPD-PCR for accurate and timely identification of C. hominivorax from other wound inhabiting flies, and to gain insight into the potential of RAPD-PCR to discriminate geographic origin of screwworm samples.…”

Karyotypic and Molecular Identification of Laboratory Stocks of the South American Fruit Fly <I>Anastrepha fraterculus</I> (Wied) (Diptera: Tephritidae)

“…Genomic DNA extractions (30 adults per stock) and RAPDs/PCR reactions were performed according to Sonvico et al (1996). The sequence of the primers (Operon Technologies, Alameda, CA) selected for the deÞnitive RAPD/PCR reactions were: P21 (5Ј-GTGAGGCGTC-3Ј) and P25 (5Ј-GATGACCGCC-3Ј).…”

“…4 and data not shown). These were considered species markers (see Sonvico et al 1996). In addition, the random primers reported here (P21 and P25) also allowed the ampliÞcation of DNA polymorphic sequence size speciÞc for each stock (Fig.…”

“…Previous studies showed variation among and within populations (Basso and Manso 1998, Sonvico et al 1996, Lifschitz et al 1999 and suggested that, at least within the Argentina limits, A. fraterculus comprises a single biological species showing many polymorphisms.…”

Morphological and molecular characterization of three species of Anastrepha Schiner and of Ceratitis capitata (Wiedemann) (Diptera: Tephritidae)

“…In New Zealand, a method for quarantine purposes, for identification of tephritids larvae to the specific level, using a molecular technique based on the analysis of RFLPs of ribosomic DNA amplified by PCR was developed (Armstrong et al 1997). Using the technique of RAPD-PCR with DNA isolated from larvae, Sonvico et al (1996) demonstrated the existence of polymorphism in C. capitata and A. fraterculus. This technique also allowed the separation of two populations of A. fraterculus originated from Argentina.…”