Karyotypic and Molecular Identification of Laboratory Stocks of the South American Fruit Fly &lt;I&gt;Anastrepha fraterculus&lt;/I&gt; (Wied) (Diptera: Tephritidae)

Basso, Alicia; Sonvico, Ariane; Quesada-Allué, Luis A.; Manso, F.

doi:10.1603/0022-0493-96.4.1237

Cited by 10 publications

(2 citation statements)

References 7 publications

(8 reference statements)

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“…Differences in the fragment size were not detected in a previous ITS1 analysis of Argentinian samples [52]. If only the size of the amplified fragment had been considered herein, we would also have found no relevant differences between the samples, but the scenario is quite different when the sequences of such fragments are considered.…”

Section: Discussionmentioning

confidence: 60%

What Can Integrated Analysis of Morphological and Genetic Data Still Reveal about the Anastrepha fraterculus (Diptera: Tephritidae) Cryptic Species Complex?

Prezotto

Perondini

Hernández‐Ortiz

et al. 2019

Insects

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The South American fruit fly Anastrepha fraterculus (Wiedemann) is a complex of cryptic species, the so-called “Anastrepha fraterculus complex”, for which eight morphotypes are currently recognized. A previous analysis of ITS1 in samples of the Anastrepha fraterculus complex, while revealing high distinctiveness among samples from different localities of South America, Central America, and Mexico, no direct association was made between sequence type and morphotype. In the present report, a correlated analysis of morphometry and ITS1 data involved individuals belonging to the same population samples. Although showing a low level of intra-populational nucleotide variability, the ITS1 analysis indicated numerous inter-population sequence type variants. Morphotypes identified by morphometric analysis based on female wing shape were highly concordant with ITS1 genetic data. The correlation of genetic divergence and morphological differences among the tested samples gives strong evidence of a robust dataset, thereby indicating the existence of various taxonomic species within the A. fraterculus complex. However, the data revealed genetic and morphological variations in some regions, suggesting that further analysis is still required for some geographic regions.

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Section: Discussionmentioning

confidence: 60%

What Can Integrated Analysis of Morphological and Genetic Data Still Reveal about the Anastrepha fraterculus (Diptera: Tephritidae) Cryptic Species Complex?

Prezotto

Perondini

Hernández‐Ortiz

et al. 2019

Insects

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show abstract

“…N-banding was obtained as described in Ref. [11]. At least 10 metaphase plates per chromosome spread were analyzed.…”

Section: Methodologiesmentioning

confidence: 99%

Reference Karyotypes and Chromosomal Variability: A Journey with Fruit Flies and the Key to Survival

Abraham¹

2017

Chromosomal Abnormalities - A Hallmark Manifestation of Genomic Instability

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My goal is to present the analysis of concepts on the "normal" karyotype and chromosomal abnormalities through comprehension of chromosomal variation within fruit flies populations, advantages of these insects as models to study genetic polymorphisms and the methodology from field to strains. Cytological preparations were obtained from cerebral ganglion. Staining methods include routine and fluorescent bandings as well as in situ hybridization using DNA probes. We define a more frequent karyotype of each one species and take them as the reference karyotype. The reference as well as the chromosomal variants studied within each species were isolated in different strains. The techniques applied revealed differences among individuals belonging to different strains, thus documenting the mutations into the DNAr cluster, variation in the patterns of heterochromatin, mosaic specimens carrying nuclei with different chromosomal numbers. Hoecht revealed double-minute chromosomes and CG-rich banding marked somatic crossing over between sister chromatids. The most frequent karyotype is the reference karyotype, namely, the normal karyotype. Chromosomal mutations produce variability. In man, a number of these mutants are considered chromosomal abnormalities. We learnt that variation is the key to survival and that many individuals could be in the right place in the wrong moment.

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Phylogenetic relationships of Andean-Ecuadorian populations ofAnastrepha fraterculus(Wiedemann 1830) (Diptera: Tephritidae) inferred from COI and COII gene sequences

Ludeña

Bayas

Pintaud

2010

Annales de la Société entomologique de France (N.S.)

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Abstract. Phylogenetic relationships among Andean-Ecuadorian and other Neotropical populations ofAnastrepha fraterculus and related species have been studied using two regions of mtDNA : 405 base pairs within Cytochrome Oxidase I ( COI) and 224 base pairs within Cytochrome Oxidase II (COII). Phylogenetic relationships were inferred using Maximun Parsimony (MP) method and haplotype networks. Andean-Ecuadorian populations of A. fraterculus are monomorphic at the COI locus and fall within a clade of South-American lowland populations of A. fraterculus. They appear to be unrelated with populations of northern Andes of Colombia and Venezuela also assigned to A. fraterculus, meaning that this species, as currently circumscribed, is not monophyletic and is composed of different biological entities that are little differentiated morphologically. At the COII locus, AndeanEcuadorian populations of A. fraterculus show a major haplotype with a few variants, and form a clade with the lowland populations of southern Brazil an Argentina, but are clearly differentiated from them. Andean-Ecuadorian populations of Anastrepha fraterculus appear to be homogeneous with respect to their mitochondrial genome and thus their identity as members of a single gene pool is confi rmed by these results. Résumé. Relations phylogénétiques entres les populations des Andes d'Equateur d'Anastrepha

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Karyotypic and Molecular Identification of Laboratory Stocks of the South American Fruit Fly <I>Anastrepha fraterculus</I> (Wied) (Diptera: Tephritidae)

Cited by 10 publications

References 7 publications

What Can Integrated Analysis of Morphological and Genetic Data Still Reveal about the Anastrepha fraterculus (Diptera: Tephritidae) Cryptic Species Complex?

What Can Integrated Analysis of Morphological and Genetic Data Still Reveal about the Anastrepha fraterculus (Diptera: Tephritidae) Cryptic Species Complex?

Reference Karyotypes and Chromosomal Variability: A Journey with Fruit Flies and the Key to Survival

Phylogenetic relationships of Andean-Ecuadorian populations ofAnastrepha fraterculus(Wiedemann 1830) (Diptera: Tephritidae) inferred from COI and COII gene sequences

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