Discrepancies between the fate of myoblast xenograft in mouse leg muscle and NMR label persistency after loading with Gd-DTPA or SPIOs

Baligand, Céline; Vauchez, Karine; Fiszman, Marc; Vilquin, Jean‐Thomas; Pg, Carlier

doi:10.1038/gt.2009.12

Cited by 23 publications

(19 citation statements)

References 46 publications

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“…This imaging signal mimics successful cell engrafting but actually represents cell death. The problem is well recognized, also with other imaging techniques as magnetic cell labeling for MR imaging [76], [77], [78], [79]. Some studies have thus focused on generating a functional imaging signal to detect death of the implanted cells be compartmentalization effects [80].…”

Section: Discussionmentioning

confidence: 99%

Detection of intramyocardially injected DiR-labeled mesenchymal stem cells by optical and optoacoustic tomography

et al. 2017

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The distribution of intramyocardially injected rabbit MSCs, labeled with the near-infrared dye 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricarbo-cyanine-iodide (DiR) using hybrid Fluorescence Molecular Tomography-X-ray Computed Tomography (FMT-XCT) and Multispectral Optoacoustic Tomography (MSOT) imaging technologies, was investigated.Viability and induction of apoptosis of DiR labeled MSCs were assessed by XTT- and Caspase-3/-7-testing in vitro. 2 × 106, 2 × 105 and 2 × 104 MSCs labeled with 5 and 10 μg DiR/ml were injected into fresh frozen rabbit hearts. FMT-XCT, MSOT and fluorescence cryosection imaging were performed.Concentrations up to 10 μg DiR/ml did not cause apoptosis in vitro (p > 0.05). FMT and MSOT imaging of labeled MSCs led to a strong signal. The imaging modalities highlighted a difference in cell distribution and concentration correlated to the number of injected cells. Ex-vivo cryosectioning confirmed the molecular fluorescence signal.FMT and MSOT are sensitive imaging techniques offering high-anatomic resolution in terms of detection and distribution of intramyocardially injected stem cells in a rabbit model.

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Section: Discussionmentioning

confidence: 99%

Detection of intramyocardially injected DiR-labeled mesenchymal stem cells by optical and optoacoustic tomography

et al. 2017

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“…Recent studies on the heart and leg showed that magnetic nanoparticles were not cleared quickly after cell death, with the cellular viability being confirmed by other methods, indicating that in certain cases, the persistent MR signals may be misleading. [160][161][162] Given that macrophages would phagocytize dead or dying SPIO-labeled cells, the endogenous macrophages can be mistaken for transplanted stem cells. There can also be extracellular iron that remains in the tissue instead of being removed or taken up by endogenous cells.…”

Section: Tracing Stem Cellsmentioning

confidence: 99%

The Role of Nanotechnology in Induced Pluripotent and Embryonic Stem Cells Research

Chen¹,

Qiu²,

Li³

2014

j biomed nanotechnol

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This paper reviews the recent studies on development of nanotechnology in the field of induced pluripotent and embryonic stem cells. Stem cell therapy is a promising therapy that can improve the quality of life for patients with refractory diseases. However, this option is limited by the scarcity of tissues, ethical problem, and tumorigenicity. Nanotechnology is another promising therapy that can be used to mimic the extracellular matrix, label the implanted cells, and also can be applied in the tissue engineering. In this review, we briefly introduce implementation of nanotechnology in induced pluripotent and embryonic stem cells research. Finally, the potential application of nanotechnology in tissue engineering and regenerative medicine is also discussed.

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“…However, long-term observation has revealed that SPIO within the tissue and contrast on MRI persists despite the death of transplanted stem cells [96,144,145]. In this context, SPIOs offer robust and specific information about the distribution of labeled cells; however, long-term monitoring of the fate of stem cells requires cautious interpretation.…”

Section: Stem Cell Labels and Tracersmentioning

confidence: 99%

Personalized nanomedicine advancements for stem cell tracking

Janowski¹,

Bulte²,

Walczak³

2012

Advanced Drug Delivery Reviews

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Discrepancies between the fate of myoblast xenograft in mouse leg muscle and NMR label persistency after loading with Gd-DTPA or SPIOs

Cited by 23 publications

References 46 publications

Detection of intramyocardially injected DiR-labeled mesenchymal stem cells by optical and optoacoustic tomography

Detection of intramyocardially injected DiR-labeled mesenchymal stem cells by optical and optoacoustic tomography

The Role of Nanotechnology in Induced Pluripotent and Embryonic Stem Cells Research

Personalized nanomedicine advancements for stem cell tracking

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