Disc electrophoresis. further modifications in the technique of sample introduction

Ka, Narayan; Narayan, Suhasini; Fa, Kummerow

doi:10.1016/0009-8981(66)90092-1

Cited by 11 publications

(3 citation statements)

References 6 publications

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“…Electrophoretic and ultracentrifugal procedures. The protein and lipoprotein patterns in 3.75% polyacrylamide gel were obtained on individual serum samples with a model 12 disk electrophoresis equipment (Canalco, Rockville, Md., U.S.A.) according to procedures previously described (Narayan et al 1966a;Narayan, Narayan & Kummerow, 1966c). The lipoproteins were stained with Sudan Black B and densitometer traces of the gel patterns were obtained by using a Canalco model E microdensitometer.…”

Section: Methodsmentioning

confidence: 99%

Serum lipoproteins during chemical carcinogenesis

Narayan¹

1967

Biochemical Journal

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1. The serum-protein and -lipoprotein patterns of rats, given a purified diet containing 0%, 0.005% or 0.03% 2-acetamidofluorene, have been determined by disk electrophoresis. 2. An abnormal high-density lipoprotein was detected from the first week of the experiment in the serum of the animals given the carcinogen. The low-density lipoproteins also increased on prolonged feeding with the carcinogen.

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Section: Methodsmentioning

confidence: 99%

Serum lipoproteins during chemical carcinogenesis

Narayan¹

1967

Biochemical Journal

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“…Plasma protein distribution of maternal and fetal blood was examined by the electrophoretic method of Narayan (16,17) and total plasma protein of the same samples was determined by the method of Lowry et ol. (13).…”

Section: Electrophoresismentioning

confidence: 99%

Magnesium Deficiency Anemia in the Rat Fetus

et al. 1977

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“…The disc electrophoretic procedure was the same as method C of Narayan et al (19). The acrylamide concentration of the main gel was 3.75% and its length was 1.25 in.…”

Section: Methodsmentioning

confidence: 99%

The effect of dietary supplementation of cholesterol and its subsequent withdrawal on the liver lipids and serum lipoproteins of chickens

Kruski

Narayan

1972

Lipids

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Two groups of male chickens were fed either a control diet (group N) containing a standard poultry ration admixed with 10% corn oil or a cholesterol diet (group C) in which the control diet was supplemented with 1% cholesterol. After 6 weeks on the diets, a negligible amount of very low density lipoprotein (VLDL) was found in the serum from control animals. On the other hand, the serum VLDL from the cholesterol‐fed birds was the predominant lipoprotein and carried 72% of the total serum lipids. Surprisingly this lipoprotein from cholesterol‐fed animals was very low in triglycrides (6%) and high in total cholesterol (77%). While the level of serum low density liporotein was unaffected by the ingestion of cholesterol, the concentration of total lipids and phospholipids in the high density lipoprotein decreased in cholesterol‐fed animals. The greatest change in liver lipids from animals fed cholesterol was found in the cholesterol esters, whereas the unesterified cholesterol, triglyceride and phospholipid varied slightly or remained constant. In normal animals the distribution of cholesterol between the liver and the serum was about equal, whereas in the cholesterol‐fed birds the liver accounted for 80% of the cholesterol found in the liver‐serum pool. In order to determine how the hypercholesterolemic bird responds to the withdrawal of cholesterol from the ration, a diet‐exchange experiment was conducted. In this study the birds that were originally fed the cholesterol diets (group C) for 6 weeks were placed on the control diet (group CN) and the birds fed the control diet (group N) for 6 weeks were given the cholesterol diet (group NC). At periodic intervals, 1, 3, 7 and 14 days following the change of diets, 3–5 animals from each group were sacrificed, and analyses performed on their serum lipoproteins and liver lipids. Within one day after the diet substitution, there was a 31‐fold increase and a 46% decrease, respectively, in the serum VLDL concentration in groups NC and CN as compared with their corresponding steady state values (groups N and group C, respectively). The liver cholesterol increased 4‐fold and decreased 40%, respectively, in the two groups NC and CN as compared with the values obtained before the diet substitution. It is suggested that the concentration of cholesterol in the liver is the principal factor controlling cholesterol metabolism in chickens fed a hypercholesterolemic diet.

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Disc electrophoresis. further modifications in the technique of sample introduction

Cited by 11 publications

References 6 publications

Serum lipoproteins during chemical carcinogenesis

Serum lipoproteins during chemical carcinogenesis

Magnesium Deficiency Anemia in the Rat Fetus

The effect of dietary supplementation of cholesterol and its subsequent withdrawal on the liver lipids and serum lipoproteins of chickens

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