Directly coupled liquid chromatography/fluorine-19 Fourier transform nuclear magnetic resonance

Analysis of antimycin A by reversed-phase liquid chromatography/nuclear magnetic resonance spectrometry

“…Like many other ternary reactions, the reaction can be used to determine any of the three components involved. Thiols have been the analytes of interest for Nakamura and Tamura (10) and Mopper and Delmas (11). The latter authors also showed that sulfite and sulfide react in the same fashion as thiols.…”

“…Analysis of the antimycin A mixture, using the optimal analytical high-performance liquid chromatography/nuclear magnetic resonance conditions, reveals It to consist of at least 10 closely related components.A direct-linked stopped-flow high-performance liquid chromatography/nuclear magnetic resonance (HPLC/NMR) system was first demonstrated for mixture analysis by Watanabe and Niki in 1976 (1). Subsequently, direct linkage of HPLC flow systems with proton nuclear magnetic resonance spectrometers has been a subject of active research in a number of laboratories (2)(3)(4)(5)(6)(7)(8)(9)(10)(11). Such an on-line technique provides both chromatographic separation and spectral information simultaneously and can be quite valuable, provided the sensitivity limitations of NMR are tolerable.…”

Process NMR spectrometry

“…The major advantage of 19 F NMR, in comparison to 1 H NMR, is that, for the most commonly used solvents, there will be no large solvent peak present in the sample spectrum. 22,51 HPLC- 19 F NMR spectrometry has been used to examine mixtures of p-fluorobenzoate derivatives of simple alcohols and phenols, 51 and has found application in the pharmaceutical industry where many drugs contain fluorine.…”

Hyphenation of high-performance liquid chromatography (HPLC) and other chromatographic techniques (SFC, GPC, GC, CE) with nuclear magnetic resonance (NMR): A review

“…Allen et al [43] described the suitability of F NMR, using mixtures of p-fluorobenzoate derivatives of some alcohols and phenols.…”