Analysis of antimycin A by reversed-phase liquid chromatography/nuclear magnetic resonance spectrometry

Ha, Steven T. K.; Wilkins, Charles L.; Abidi, S. L.

doi:10.1021/ac00180a005

Cited by 9 publications

(2 citation statements)

References 21 publications

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“…Ha et al [27] analyzed antimycin A, a mixture of closely related streptomyces fermentation products with antibiotic effects. Due to the unstability of these compounds the on-line detection is a great advantage prior to conventional analysis.…”

Section: Pharmaceutical and Clinical Chemistrymentioning

confidence: 99%

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Hyphenation of high-performance liquid chromatography (HPLC) and other chromatographic techniques (SFC, GPC, GC, CE) with nuclear magnetic resonance (NMR): A review

Korhammer

Berndt

1996

Fresenius J Anal Chem

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The development of the hyphenation of highperformance liquid chromatography (HPLC) and other chromatographic methods (supercritical fluid chromatography (SFC), gel-permeation chromatography (GPC), gas chromatography (GC) and capillary electrophoresis (CE)) with nuclear magnetic resonance (NMR) is reviewed. The focus is not only on application (e.g. pharmaceutical chemistry, food chemistry, polymer analysis, fuel chemistry), but also on technical improvements (e.g. flow rate, flow cell, probe, solvent suppression). The increasing importance and overwhelming analytical possibilities of on-line coupling of HPLC with NMR is demonstrated. The literature up to March 1995 is considered.

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Section: Pharmaceutical and Clinical Chemistrymentioning

confidence: 99%

“…Mixtures of peptides were separated and determined in a short time by Spraul [13]. Ha et al [27] analyzed mixtures of three amino acids. Laude et al [10] as well as Laude and Wilkins [38] reported on the separation of some test mixtures (e.g.…”

Section: Other Applicationsmentioning

confidence: 99%

Hyphenation of high-performance liquid chromatography (HPLC) and other chromatographic techniques (SFC, GPC, GC, CE) with nuclear magnetic resonance (NMR): A review

Korhammer

Berndt

1996

Fresenius J Anal Chem

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A novel nanospray capillary zone electrophoresis/mass spectrometry interface

Hsieh

Baronas

Muir

et al. 1999

Rapid Commun. Mass Spectrom.

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The high resolution of capillary zone electrophoresis/mass spectrometry (CZE/MS) offers a promising technique to characterize biomolecules in pharmaceutical and biotechnology industries. A novel capillary zone electrophoresis/electrospray ionization time-of-flight mass spectrometry (CZE/ESI-TOFMS) interface was designed in this study to successfully integrate ESI-TOFMS, nanospray, and CZE for biomolecular identification. The interface offers a novel way to take advantage of the high resolution separation achieved during CZE and the detection sensitivity of nanospray ESI-MS. The results showed mixtures of peptides were highly resolved within a few minutes (each CZE electropherogram of a peptide is 2-3 seconds). The novel CZE/ESI-TOFMS interface may simultaneously provide sensitivity, data acquisition speed, mass range, and mass resolution while maintaining resolution of the CZE separation.

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The antimycin A-sensitive pathway of cyclic electron flow: from 1963 to 2015

2016

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Cyclic electron flow has puzzled and divided the field of photosynthesis researchers for decades. This mainly concerns the proportion of its overall contribution to photosynthesis, as well as its components and molecular mechanism. Yet, it is irrefutable that the absence of cyclic electron flow has severe effects on plant growth. One of the two pathways mediating cyclic electron flow can be inhibited by antimycin A, a chemical that has also widely been used to characterize the mitochondrial respiratory chain. For the characterization of cyclic electron flow, antimycin A has been used since 1963, when ferredoxin was found to be the electron donor of the pathway. In 2013, antimycin A was used to identify the PGRL1/PGR5 complex as the ferredoxin:plastoquinone reductase completing the last puzzle piece of this pathway. The controversy has not ended, and here, we review the history of research on this process using the perspective of antimycin A as a crucial chemical for its characterization.

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Analysis of antimycin A by reversed-phase liquid chromatography/nuclear magnetic resonance spectrometry

Cited by 9 publications

References 21 publications

Hyphenation of high-performance liquid chromatography (HPLC) and other chromatographic techniques (SFC, GPC, GC, CE) with nuclear magnetic resonance (NMR): A review

Hyphenation of high-performance liquid chromatography (HPLC) and other chromatographic techniques (SFC, GPC, GC, CE) with nuclear magnetic resonance (NMR): A review

A novel nanospray capillary zone electrophoresis/mass spectrometry interface

The antimycin A-sensitive pathway of cyclic electron flow: from 1963 to 2015

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