The antimycin A-sensitive pathway of cyclic electron flow: from 1963 to 2015

Labs, Mathias; Rühle, Thilo; Leister, Dario

doi:10.1007/s11120-016-0217-2

Cited by 46 publications

(35 citation statements)

References 79 publications

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“…In particular, the AA‐treated leaves displayed a lower rate of CET (Fig. F), consistent with the observation that one of the paths of CET is AA‐sensitive (Joët et al , Sugimoto et al , Shikanai , Labs et al ). Measures of CET are viewed with caution because of the difficulty in quantifying this pathway flux (Johnson ).…”

Section: Discussionsupporting

confidence: 90%

Signaling interactions between mitochondria and chloroplasts in Nicotiana tabacum leaf

Alber

Vanlerberghe

2019

Physiologia Plantarum

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Research has begun to elucidate the signal transduction pathway(s) that control cellular responses to changes in mitochondrial status. Important tools in such studies are chemical inhibitors used to initiate mitochondrial dysfunction. This study compares the effect of different inhibitors and treatment conditions on the transcript amount of nuclear genes specifically responsive to mitochondrial dysfunction in leaf of Nicotiana tabacum L. cv. Petit Havana. The Complex III inhibitors antimycin A (AA) and myxothiazol (MYXO), and the Complex V inhibitor oligomycin (OLIGO), each increased the transcript amount of the mitochondrial dysfunction genes. Transcript responses to OLIGO were greater during treatment in the dark than in the light, and the dark treatment resulted in cell death. In the dark, transcript responses to AA and MYXO were similar to one another, despite MYXO leading to cell death. In the light, transcript responses to AA and MYXO diverged, despite cell viability remaining high with either inhibitor. This divergent response may be due to differential signaling from the chloroplast because only AA also inhibited cyclic electron transport, resulting in a strong acceptor‐side limitation in photosystem I. In the light, chemical inhibition of chloroplast electron transport reduced transcript responses to AA, while having no effect on the response to MYXO, and increasing the response to OLIGO. Hence, when studying mitochondrial dysfunction signaling, different inhibitor and treatment combinations differentially affect linked processes (e.g. chloroplast function and cell fate) that then contribute to measured responses. Therefore, inhibitor and treatment conditions should be chosen to align with specific study goals.

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Section: Discussionsupporting

confidence: 90%

Signaling interactions between mitochondria and chloroplasts in Nicotiana tabacum leaf

Alber

Vanlerberghe

2019

Physiologia Plantarum

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“…5c), suggesting that the change in PSI redox status in Scots pine during the spring-to-summer transition may be linked to the changes in PGR5 abundance. To confirm the contribution of PGR5, the second experiment was performed where the dynamic FL protocol was combined with antimycin A (AA) treatment, which has been extensively characterized as an inhibitor of PGR5dependent CET 31 . Samples from Scots pine and Norway spruce were collected in the field in April, and Y(I) was determined following AA treatment and following a recovery period of 24 h at room temperature (Fig.…”

Section: Resultsmentioning

confidence: 99%

Two dominant boreal conifers use contrasting mechanisms to reactivate photosynthesis in the spring

et al. 2020

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Boreal forests are dominated by evergreen conifers that show strongly regulated seasonal photosynthetic activity. Understanding the mechanisms behind seasonal modulation of photosynthesis is crucial for predicting how these forests will respond to changes in seasonal patterns and how this will affect their role in the terrestrial carbon cycle. We demonstrate that the two co-occurring dominant boreal conifers, Scots pine (Pinus sylvestris L.) and Norway spruce (Picea abies), use contrasting mechanisms to reactivate photosynthesis in the spring. Scots pine downregulates its capacity for CO 2 assimilation during winter and activates alternative electron sinks through accumulation of PGR5 and PGRL1 during early spring until the capacity for CO 2 assimilation is recovered. In contrast, Norway spruce lacks this ability to actively switch between different electron sinks over the year and as a consequence suffers severe photooxidative damage during the critical spring period.

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“…Other important photochemical processes related to photoprotection include a) reduction in light capture through the decrease in amount of antenna components and b) excess energy dissipation via thermal component of non-photochemical quenching (qE), which is dependent on the thylakoid ΔpH, the amount of PsbS protein and the zeaxanthin-violaxanthin balance via violaxanthin de-epoxidase activity (Ruban, 2017). In addition, the equilibrium between the photochemical activities of PSII and PSI and the regulation of cyclic electron flow are also very important as photoprotective mechanisms (Finazzi and Johnson, 2016;Labs et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant protection and PSII regulation mitigate photo-oxidative stress induced by drought followed by high light in cashew plants

Lima

Ferreira‐Silva

Carvalho

et al. 2018

Environmental and Experimental Botany

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We tested the hypothesis that effective antioxidant and photoprotective mechanisms are able to avoid photodamage induced by prolonged water deficit (WD) followed by high light (HL). We employed cashew plants (Anacardium occidentale L.), a semiarid adapted species, as a model plant. WD-plants exposed to HL did not show alterations in maximum quantum efficiency of photosystem II (Fv/Fm), cellular integrity, H 2 O 2 and thiobarbituric acid reactive species (TBARS) contents, evidencing that they did not suffered photoinhibition and oxidative stress. These responses were associated with increases in ascorbate peroxidase (APX) and superoxide dismutase (SOD) activities, glutathione (GSH) oxidation and ascorbate (ASC) synthesis. This effective oxidative protection occurred in parallel to strong decrease in photosystem II and I (PSII and PSI) activities, increase in heat dissipation (qE), which was related to enhancement in cyclic electron flux. These favorable photoprotective changes were associated with efficient water use in response to HL, all contributing to avoid excess energy in chloroplasts of drought-exposed leaves. These protective features were associated with a tight regulation in D1 protein accumulation during HL, contributing to avoid reactive oxygen species over-accumulation and a subsequent effective PSII recovery during darkness. Our results indicate that cashew plants are able to avoid photoinhibition, tolerating extreme conditions of drought combined with HL. The displayed mechanisms involve essentially integrated responses to balance energy input and output, avoiding oxidative stress.

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The antimycin A-sensitive pathway of cyclic electron flow: from 1963 to 2015

Cited by 46 publications

References 79 publications

Signaling interactions between mitochondria and chloroplasts in Nicotiana tabacum leaf

Signaling interactions between mitochondria and chloroplasts in Nicotiana tabacum leaf

Two dominant boreal conifers use contrasting mechanisms to reactivate photosynthesis in the spring

Antioxidant protection and PSII regulation mitigate photo-oxidative stress induced by drought followed by high light in cashew plants

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