Direct Synthesis of Free α-Amino Acids by Telescoping Three-Step Process from 1,2-Diols

Inada, Haruki; Shibuya, Masabumi; Yamamoto, Yoshihiko

doi:10.1021/acs.orglett.8b03910

Cited by 9 publications

(1 citation statement)

References 43 publications

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“…Another substantial species present was Unk3. HSQC and HMBC data revealed an amino acid backbone, however the side chain was revealed via COSY and TOCSY to be a simple ethyl group like homo-alanine (hAla) (Inada et al, 2019). Its presence could not be detected by LC-MS, so this metabolite remains unknown.…”

Section: Excretion Of Organic Compoundsmentioning

confidence: 99%

“Candidatus Hydrogenisulfobacillus filiaventi” strain R50 gen. nov. sp. nov., a highly efficient producer of extracellular organic compounds from H2 and CO2

et al. 2023

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Production of organic molecules is largely depending on fossil fuels. A sustainable alternative would be the synthesis of these compounds from CO2 and a cheap energy source, such as H2, CH4, NH3, CO, sulfur compounds or iron(II). Volcanic and geothermal areas are rich in CO2 and reduced inorganic gasses and therefore habitats where novel chemolithoautotrophic microorganisms for the synthesis of organic compounds could be discovered. Here we describe “Candidatus Hydrogenisulfobacillus filiaventi” R50 gen. nov., sp. nov., a thermoacidophilic, autotrophic H2-oxidizing microorganism, that fixed CO2 and excreted no less than 0.54 mol organic carbon per mole fixed CO2. Extensive metabolomics and NMR analyses revealed that Val, Ala and Ile are the most dominant form of excreted organic carbon while the aromatic amino acids Tyr and Phe, and Glu and Lys were present at much lower concentrations. In addition to these proteinogenic amino acids, the excreted carbon consisted of homoserine lactone, homoserine and an unidentified amino acid. The biological role of the excretion remains uncertain. In the laboratory, we noticed the production under high growth rates (0.034 h−1, doubling time of 20 h) in combination with O2-limitation, which will most likely not occur in the natural habitat of this strain. Nevertheless, this large production of extracellular organic molecules from CO2 may open possibilities to use chemolithoautotrophic microorganisms for the sustainable production of important biomolecules.

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Section: Excretion Of Organic Compoundsmentioning

confidence: 99%

“Candidatus Hydrogenisulfobacillus filiaventi” strain R50 gen. nov. sp. nov., a highly efficient producer of extracellular organic compounds from H2 and CO2

et al. 2023

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Genetic Code Expansion Facilitates Position‐Selective Modification of Nucleic Acids and Proteins

et al. 2020

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Transcription and translation obey to the genetic code of four nucleobases and 21 amino acids evolved over billions of years. Both these processes have been engineered to facilitate the use of non‐natural building blocks in both nucleic acids and proteins, enabling researchers with a decent toolbox for structural and functional analyses. Here, we review the most common approaches for how labeling of both nucleic acids as well as proteins in a site‐selective fashion with either modifiable building blocks or spectroscopic probes can be facilitated by genetic code expansion. We emphasize methodological approaches and how these can be adapted for specific modifications, both during as well as after biomolecule synthesis. These modifications can facilitate, for example, a number of different spectroscopic analysis techniques and can under specific circumstances even be used in combination.

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Cu/Ni-doped sulfated zirconium oxide immobilized on CdFe2O4 NPs: a cheap, sustainable and magnetically recyclable inorgano-catalyst for the efficient preparation of α-aminonitriles in aqueous media

et al. 2020

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Direct Synthesis of Free α-Amino Acids by Telescoping Three-Step Process from 1,2-Diols

Cited by 9 publications

References 43 publications

“Candidatus Hydrogenisulfobacillus filiaventi” strain R50 gen. nov. sp. nov., a highly efficient producer of extracellular organic compounds from H2 and CO2

“Candidatus Hydrogenisulfobacillus filiaventi” strain R50 gen. nov. sp. nov., a highly efficient producer of extracellular organic compounds from H2 and CO2

Genetic Code Expansion Facilitates Position‐Selective Modification of Nucleic Acids and Proteins

Cu/Ni-doped sulfated zirconium oxide immobilized on CdFe2O4 NPs: a cheap, sustainable and magnetically recyclable inorgano-catalyst for the efficient preparation of α-aminonitriles in aqueous media

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