Direct nuclear magnetic resonance identification and quantification of geometric isomers of conjugated linoleic acid in milk lipid fraction without derivatization steps: Overcoming sensitivity and resolution barriers

Tsiafoulis, Constantinos G.; Skarlas, Theodore; Tzamaloukas, Ouranios; Miltiadou, Despoina; Gerothanassis, Ioannis P.

doi:10.1016/j.aca.2014.03.010

Cited by 19 publications

(28 citation statements)

References 30 publications

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“…NMR has the ability to serve as an invaluable tool for the compositional analysis of foods, not only due to its ability to provide important information for structural elucidation, but also for its quantitative nature. NMR has been effectively used for the analysis of several classes of compounds including lipids (Dais & Hatzakis, ; Monakhova & Diehl, , ; Siddiqui et al., ; Tsiafoulis et al., ), carbohydrates (Kazalaki et al., ; Merkx, Hong, Ermacora, & van, ; Singh, Kumar, Srivastava, Deepak, & Singh, ; Spraul et al., , ), amino acids (Belton et al., ), alcohols (Hatzakis, Archavlis, & Dais, ; Nilsson et al., ), organic acids (Berregi, del Campo, Caracena, & Miranda, ), polyphenols (Agiomyrgianaki & Dais, , ; Charisiadis, Exarchou, Troganis, & Gerothanassis, ), vitamins (Ackermann et al., ; Dais et al., ; Shumilina, Ciampa, Capozzi, Rustad, & Dikiy, ; Vaysse et al., ), terpenes (Dais, Plessel, Williamson, & Hatzakis, ), phospholipids (Hatzakis, Koidis, Boskou, & Dais, ; Kaffarnik, Ehlers, Gröbner, Schleucher, & Vetter, ), colorants (Englert, ; Scotter, ; Venkatachalam et al., ; Berké, Chèze, Vercauteren, & Deffieux, ), and contaminants (Lachenmeier et al., ) in a variety of foods, such as oils (Dais & Hatzakis, ; Siddiqui et al., ), beverages (Kidrič, ; Ryu, Koda, Miyaka, & Tanokura, ; Santos, Fonseca, Lião, Alcantara, & Barison, ; Zurriarrain et al., 2015), meats (García‐García et al., ), dairy products (Gangwar, Singh, & Deepak, ; Hu, Furihata, Ito‐Ishida, Kaminogawa, & Tanokura, ; Maher & Rochfort, ; Murgia, Mele, & Monduzzi, ; Scano et al., ; Tociu et al., ), and infant formula (Zhao et al., ). For the structural characterization of various food components, the application of 2D techniques is often required.…”

Section: Fundamentals Of Nmr Spectroscopy‐relevance To Food Sciencementioning

confidence: 99%

Nuclear Magnetic Resonance (NMR) Spectroscopy in Food Science: A Comprehensive Review

Hatzakis

2018

Comp Rev Food Sci Food Safe

255

126

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Nuclear magnetic resonance (NMR) spectroscopy is a robust method, which can rapidly analyze mixtures at the molecular level without requiring separation and/or purification steps, making it ideal for applications in food science. Despite its increasing popularity among food scientists, NMR is still an underutilized methodology in this area, mainly due to its high cost, relatively low sensitivity, and the lack of NMR expertise by many food scientists. The aim of this review is to help bridge the knowledge gap that may exist when attempting to apply NMR methodologies to the field of food science. We begin by covering the basic principles required to apply NMR to the study of foods and nutrients. A description of the discipline of chemometrics is provided, as the combination of NMR with multivariate statistical analysis is a powerful approach for addressing modern challenges in food science. Furthermore, a comprehensive overview of recent and key applications in the areas of compositional analysis, food authentication, quality control, and human nutrition is provided. In addition to standard NMR techniques, more sophisticated NMR applications are also presented, although limitations, gaps, and potentials are discussed. We hope this review will help scientists gain some of the knowledge required to apply the powerful methodology of NMR to the rich and diverse field of food science.

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Section: Fundamentals Of Nmr Spectroscopy‐relevance To Food Sciencementioning

confidence: 99%

Nuclear Magnetic Resonance (NMR) Spectroscopy in Food Science: A Comprehensive Review

Hatzakis

2018

Comp Rev Food Sci Food Safe

255

126

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“…In previous studies, the lipid profile of milk and dairy products has been studied by high-resolution NMR spectroscopy for differentiating the animal provenience [20][21][22][23], while other studies reported the presence of different CLA isomers [24][25][26].…”

Section: Introductionmentioning

confidence: 99%

NMR Lipid Profile of Milk from Alpine Goats with Supplemented Hempseed and Linseed Diets

et al. 2020

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The supplementation of goat diets with natural products to obtain milk with nutraceutical components is a common practice. In these last years, the influence of supplementation of specifically designed diets has been studied with different analytical tools in order to explore possible beneficial effects in human consumption of animal milk and milk-derived products. In this study, the lipid fraction of milk from Alpine goats undergoing different dietary regimens was studied by 1H-NMR spectroscopy. Alpine goats were fed with linseed or hempseed supplements, and after 14 weeks of treatment, milk was collected and analyzed. Results showed that feeding diets supplemented with seeds positively affected the fatty acid composition with a pronounced increase in unsaturated fatty acids for both diets compared to a control diet. Specifically, linolenic acid content was more than doubled for linseed diet compared with the hempseed and control groups, while linoleic acid greatly increased only upon hempseed supplementation. However, a number of conjugated linoleic acid (CLA) isomers and higher levels of fatty acids with trans configuration were found in supplemented diets, particularly in the linseed diet.

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“…Among the spectroscopic methods commonly used in the analysis of natural products, NMR spectroscopy is a dominant technique due to the wide range of experimental approaches that are available, the high level of structural information that is provided and the non-destruction of the sample (Novoa-Carballal et al, 2011;Pauli et al, 2012). Recently, significant effort has been given to the development of new NMR methodologies that can be applied to the analysis of various constituents of complex plant extracts without any previous separation or isolation of the individual components including two-dimensional (2D) 1 H-1 H, 1 H-13 C HSQC and 1 H-13 C HMBC NMR experiments (Lewis et al, 2007;Tsiafoulis et al, 2014), DOSY-NMR (Rodriques et al, 2009;Prieto et al, 2016), maximum-quantum (Manjunatha Reddy and Caldarelli, 2011), selective 1D TOCSY (Sandusky and Raftery, 2005;Papaemmanouil et al, 2015), and broadband 1 H homodecoupled (pure shift) NMR (Castañar and Parella, 2015). However, the phytochemical study remains an extremely difficult task due to severe overlapping of resonances in both the aliphatic and aromatic region.…”

Section: Introductionmentioning

confidence: 99%

Determination of Polyphenolic Phytochemicals using Highly Deshielded –OH ¹H‐NMR Signals

Charisiadis

Kontogianni

Tsiafoulis

et al. 2016

Phytochemical Analysis

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Introduction Quantitative analysis of plant extracts is a tedious and time consuming process requiring chromatographic techniques and/or the use of specilised hyphenated spectroscopic methods. Thus, investigation of a rapid method capable for the determination of bioactive constituents is of major importance. Objective To employ high resolution 1H‐NMR spectroscopy of the –OH spectral region, as a rapid method for the simultaneous determination and quantification of polyphenolic phytochemicals in crude oregano and olive leaf extracts without any prior isolation step. Methodology A critical overview of experimental parameters that influence the resolution of the –OH groups is provided with emphasis on the nature of the extraction solvent, effects of dilution, pH, temperature and nature of the NMR solvent. The compounds were assigned with the use of 1H‐NMR, 2D 1H‐13C HMBC NMR and spiking experiments with model compounds. Results The method was applied for the NMR analysis of natural product extracts and the results were found to be in good agreement with those obtained with the use of the time consuming HPLC‐DAD, preparative LC and LC‐SPE‐NMR. Conclusion The method is rapid, selective, with very good analytical performance characteristics, which support the efficiency of the suggested methodology. Copyright © 2016 John Wiley & Sons, Ltd.

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Direct nuclear magnetic resonance identification and quantification of geometric isomers of conjugated linoleic acid in milk lipid fraction without derivatization steps: Overcoming sensitivity and resolution barriers

Cited by 19 publications

References 30 publications

Nuclear Magnetic Resonance (NMR) Spectroscopy in Food Science: A Comprehensive Review

Nuclear Magnetic Resonance (NMR) Spectroscopy in Food Science: A Comprehensive Review

NMR Lipid Profile of Milk from Alpine Goats with Supplemented Hempseed and Linseed Diets

Determination of Polyphenolic Phytochemicals using Highly Deshielded –OH ¹H‐NMR Signals

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Direct nuclear magnetic resonance identification and quantification of geometric isomers of conjugated linoleic acid in milk lipid fraction without derivatization steps: Overcoming sensitivity and resolution barriers

Cited by 19 publications

References 30 publications

Nuclear Magnetic Resonance (NMR) Spectroscopy in Food Science: A Comprehensive Review

Nuclear Magnetic Resonance (NMR) Spectroscopy in Food Science: A Comprehensive Review

NMR Lipid Profile of Milk from Alpine Goats with Supplemented Hempseed and Linseed Diets

Determination of Polyphenolic Phytochemicals using Highly Deshielded –OH 1H‐NMR Signals

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Product

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About

Determination of Polyphenolic Phytochemicals using Highly Deshielded –OH ¹H‐NMR Signals