Direct Imaging of the Stepwise Elution Process in High-Performance Liquid Chromatography

Peak crossover in high-performance liquid chromatography elution monitored using whole-column detection

“…As time elapsed, the moving peak split into another peak with an apparent shoulder. The relationship between the migration time and migration distance in a stage-wise gradient elution could also be predicted using the following equation with chromatographic data obtained by the post-column detector [11,12]:…”

Spatio-temporally resolved detection on a microfluidic chip for monitoring the dynamic processes of molecular events

“…21 It was also found to be useful for investigation of the stepwise elution process in HPLC. 22 Although PCID cannot provide the whole picture of the separation dynamics in the entire column, the dynamic information obtained is still very useful. Whole column image detection (WCID) [23][24][25][26][27][28] is a unique detection scheme particularly invented for CIEF, though it can also be useful for other CE modes.…”