Spatio-temporally resolved detection on a microfluidic chip for monitoring the dynamic processes of molecular events

Bi, Xiaodong; Yu, Jianzhao; Li, Li; Jiang, Hancheng; Huang, Fengliang; Liu, Zhen

doi:10.1039/c2an35650c

Cited by 7 publications

(3 citation statements)

References 40 publications

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“…On the other hand, the k off values for the MIP layers ranged from 10 À5 to 10 À4 s À1 , which are similar to those for other specic binding (10 À4 to 10 À3 s À1 ), 24,25 but lower than those for non-specic binding (10 À2 to 10 À3 s À1 ). [25][26][27] These results suggest that the specic affinity of the MIP layers towards the template was due to the well-fabricated imprinting cavities, rather than non-specic adsorption by the imprinting coating. The binding data shown in Table 1 reveal that even at pH 3, at which boronate affinity interaction was inactivated, the MIP layer still exhibited a strong enough binding strength, reaching the 10 À7 M level.…”

Section: Resultsmentioning

confidence: 97%

Affinity-tunable specific recognition of glycoproteins via boronate affinity-based controllable oriented surface imprinting

et al. 2014

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Section: Resultsmentioning

confidence: 97%

Affinity-tunable specific recognition of glycoproteins via boronate affinity-based controllable oriented surface imprinting

et al. 2014

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“…As a high-performance separation tool, capillary electrophoresis (CE) provides multiple advantages, including high resolution, speed, easy to automation, minute sample amount requirements, and the possibility to work under near-physiological conditions. These benefits make CE a powerful tool for the characterization of biomolecular interactions. − CE has been widely used in investigation of the interactions between small molecules, , drugs and proteins, − DNA and proteins, − and proteins and proteins, as well as between protein and nanoparticle . Although boronic acids have already been frequently used in CE for the separation and detection of sugar or sugar alcohols , and separation of nucleosides and glycoproteins, to the best of our knowledge, there is no particular work using CE to probe the interactions between boronic acids and cis -diol-containing biomolecules.…”

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confidence: 99%

Probing the Interactions between Boronic Acids and cis-Diol-Containing Biomolecules by Affinity Capillary Electrophoresis

et al. 2013

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The affinity of boronic acids to cis-diol-containing biomolecules has found wide applications in many fields, such as sensing, separation, drug delivery, and functional materials. A sound understanding of the binding interactions will greatly facilitate exquisite applications of this chemistry. Although a few analytical tools have been available for the characterization of the interactions, these techniques are associated with some apparent drawbacks, so they are only applicable to a limited range of boronic acids and cis-diol-containing biomolecules. Therefore, a widely applicable method is still greatly needed. In this work, an affinity capillary electrophoresis (ACE) method was established and validated to probe the interactions between boronic acids and cis-diol-containing biomolecules. The method was proven to be applicable to almost all types of cis-diol-containing biomolecules and boronic acids. Based on this method, a quantitative, comparative study on the interactions between 14 boronic acids that have important potentials for application with 5 typical monosaccharides of biological importance was carried out. The findings provided new insights into boronate affinity interactions, particularly the relationship between the binding strength with the molecular structures of the binding species. Besides, effects of pH and temperature on the binding strength were also investigated. This method exhibited several significant advantages, including (1) possibility of simultaneous study of multiple interactions, (2) low requirement on the purity of the binding species, (3) wide applicability, and (4) high accuracy and precision.

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“…Besides, the k off values for the MIP, the cMIP and the anti‐B2M monoclonal antibody ranged from 10 −4 to 10 −3 s −1 , which are similar to those for other specific binding, but lower than those for nonspecific binding (10 −2 to 10 −3 s −1 ) . [ 41‐44 ] These results suggest that the specific affinity of the MIP and cMIP toward the template was due to the well‐fabricated imprinting cavities, rather than nonspecific adsorption by the imprinting coating.…”

Section: Resultsmentioning

confidence: 97%

Controllable Engineering and Functionalizing of Nanoparticles for Targeting Specific Proteins towards Biomedical Applications

et al. 2021

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Nanoparticles have been widely used in important biomedical applications such as imaging, drug delivery, and disease therapy, in which targeting toward specific proteins is often essential. However, current targeting strategies mainly rely on surface modification with bioligands, which not only often fail to provide desired properties but also remain challenging. Here an unprecedented approach is reported, called reverse microemulsion‐confined epitope‐oriented surface imprinting and cladding (ROSIC), for facile, versatile, and controllable engineering coreless and core/shell nanoparticles with tunable monodispersed size as well as specific targeting capability toward proteins and peptides. Via engineering coreless imprinted and cladded silica nanoparticles, the effectiveness and superiority over conventional imprinting of the proposed approach are first verified. The prepared nanoparticles exhibit both high specificity and high affinity. Using quantum dots, superparamagnetic nanoparticles, silver nanoparticles, and upconverting nanoparticles as a representative set of core substrates, a variety of imprinted and cladded single‐core/shell nanoparticles are then successfully prepared. Finally, using imprinted and cladded fluorescent nanoparticles as probes, in vitro targeted imaging of triple‐negative breast cancer (TNBC) cells and in vivo targeted imaging of TNBC‐bearing mice are achieved. This approach opens a new avenue to engineering of nanoparticles for targeting specific proteins, holding great prospects in biomedical applications.

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Spatio-temporally resolved detection on a microfluidic chip for monitoring the dynamic processes of molecular events

Cited by 7 publications

References 40 publications

Affinity-tunable specific recognition of glycoproteins via boronate affinity-based controllable oriented surface imprinting

Affinity-tunable specific recognition of glycoproteins via boronate affinity-based controllable oriented surface imprinting

Probing the Interactions between Boronic Acids and cis-Diol-Containing Biomolecules by Affinity Capillary Electrophoresis

Controllable Engineering and Functionalizing of Nanoparticles for Targeting Specific Proteins towards Biomedical Applications

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