Whole-column imaging-detection techniques and their analytical applications

Wu, Xing-Zheng; Huang, Tiemin; Liu, Zhen; Pawliszyn, Janusz

doi:10.1016/j.trac.2005.02.004

Cited by 35 publications

(31 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[111,112,162]. The 5 cm section of the FS capillary with removed polyimide coating is illumi-nated by the light guided from the light source (deuterium lamp, xenon lamp, He-Ne laser, diode laser) by an optical fiber bundle and focused by cylindrical lens to the capillary.…”

Section: Uv Absorption and Interferometrymentioning

confidence: 99%

“…CIEF with the liquid-core waveguide LIF whole-column detection described in more detail in the above Section 6.1 on UV-absorption detection has been applied for separation and analysis of several different proteins and polypeptides, for microheterogeneity characterization, and pI determination of antibodies, glycoproteins, and viruses [111,112,162].…”

Section: Lifmentioning

confidence: 99%

See 1 more Smart Citation

Recent developments in CE and CEC of peptides

Kašička

2007

Electrophoresis

118

View full text Add to dashboard Cite

The article brings a comprehensive survey of recent developments and applications of high-performance capillary electromigration methods, zone electrophoresis, ITP, IEF, affinity electrophoresis, EKC, and electrochromatography, to analysis, preparation, and physicochemical characterization of peptides. New approaches to the theoretical description and experimental verification of electromigration behavior of peptides and to methodology of their separations, such as sample preparation, adsorption suppression, and detection, are presented. Novel developments in individual CE and CEC modes are shown and several types of their applications to peptide analysis are presented: conventional qualitative and quantitative analysis, purity control, determination in biomatrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid and sequence analysis, and peptide mapping of proteins. Some examples of micropreparative peptide separations are given and capabilities of CE and CEC techniques to provide important physicochemical characteristics of peptides are demonstrated.

show abstract

Section: Uv Absorption and Interferometrymentioning

confidence: 99%

Section: Lifmentioning

confidence: 99%

Recent developments in CE and CEC of peptides

Kašička

2007

Electrophoresis

118

View full text Add to dashboard Cite

show abstract

“…However, by integrating the LC onto microfluidics platform has opened up the opportunity for whole column detection (Wang et al 2010;Chan et al 2014;Al Lawati et al 2014). Since the late 1980s, many reports shown great interest in whole column detection for LC as to help better understanding of the separation process within the stainless steel column (Gelderloos et al 1986;Rowlen et al 1989;Wu et al 2005). Besides that, whole column detections provide more than one result per assay as compared to the conventional post column detection.…”

Section: Introductionmentioning

confidence: 99%

A study of the in-column detection performance for chromatography separation

Leow

Chee

Patel

et al. 2015

Microfluid Nanofluid

View full text Add to dashboard Cite

This paper studies the in-column detection performance for chromatography separation. Numerical modelling was performed to investigate the detection efficiency of the electrodes that are suspended within a packed chromatography column. The results show that the density of the silica beads packed within the channel does not hinder the detection ability of the electrode. The findings have been further validated with experimental works. A 8 in-column electrode array provides the full separation progress traces of the five neurotransmitters and metabolites (adrenalin, dopamine, DOPAC, serotonin and 5-HIAA) within the column. From the in-column detection, better peak resolutions are developed though the separation column as compared to the conventional post column detection. The whole progress of the separation shows that good separation (Rs > 1) can be obtained at E5 (28.7 mm from column inlet), whereas baseline separation could be obtained at E8 (last in-column electrode) with Rs ≥ 1.5 within 60 minutes. The presented results suggest the packing materials within the column does not obstruct the efficiency of the electrodes but able to produce a good baseline separation detection as compared to conventional post column detection.

show abstract

“…The whole-column-imaged CIEF allows direct visualization of dynamic processes of the CIEF. Also, it is much faster than the conventional CIEF because the detection and separation finish at the same time [17]. The whole-column absorbance-imaged CIEF has been commercialized and widely used for pI determination, identification, and separation of proteins and peptides.…”

Section: Introductionmentioning

confidence: 99%

Study of protein–protein binding reaction by whole‐column fluorescence‐imaged CIEF

Asai

Yamaguchi

2009

Electrophoresis

Self Cite

View full text Add to dashboard Cite

Whole-column fluorescence-imaged CIEF was applied to study protein-protein binding reaction. A homemade whole-column fluorescence-imaged CIEF experimental setup was built, and its CIEF performance was evaluated with native fluorescent protein green fluorescence protein and fluorescently labeled proteins (bovine albumin, human albumin, and BSA). pIs, focusing time, detection limits, and linear quantitative range of the proteins were obtained. Furthermore, the method was used to study FITC-protein A-human IgG binding reaction. Experimental results showed that the apparent binding ratio of the FITC-protein A to human IgG was 1:2, and pI of the binding conjugates were about 6.3-6.5. No binding reaction was found between green fluorescence protein and the fluorescent-labeled proteins.

show abstract

Whole-column imaging-detection techniques and their analytical applications

Cited by 35 publications

References 52 publications

Recent developments in CE and CEC of peptides

Recent developments in CE and CEC of peptides

A study of the in-column detection performance for chromatography separation

Study of protein–protein binding reaction by whole‐column fluorescence‐imaged CIEF

Contact Info

Product

Resources

About