Direct HPLC Analysis of Quercetin and trans-Resveratrol in Red Wine, Grape, and Winemaking Byproducts

Abstract

A simple and fast reversed-phase HPLC method using diode array detection was developed and validated for the simultaneous determination of trans-resveratrol and quercetin in Sicilian red wine from the Nero d'Avola red grape variety. Investigation was also extended to the quantitative determination of resveratrol and quercetin in grape skins and winemaking byproducts obtained from the same cultivar. Samples were eluted using a C18 narrow-bore column under isocratic conditions in less than 20 min. Quantification of trans-resveratrol and quercetin in red wine was performed without any sample pretreatment, whereas the determination of these phenolic compounds in grape skins and wine pomage required a solvent extraction procedure. Linearity was demonstrated over the 0.39-12.5 and 0.45-57.6 microg/mL range for trans-resveratrol and quercetin, respectively. Detection limits in real samples were in the low ppm level (0.07 mg/L for trans-resveratrol and 0.12 mg/L for quercetin). The HPLC-UV/DAD method was applied for the routine analyses of red wine and grape skin and winemaking byproduct extracts to evaluate their trans-resveratrol and quercetin content. In particular, a very high content of quercetin was found in wine pomace, suggesting the use of this wine byproduct as a potential source of this health-promoting phenolic compound.

Analysis of trans-Resveratrol in Grapes by Micro-High Performance Liquid Chromatography

“…[16][17][18]34 In accordance with data published by Okuda and Yokotsuka 18 and Soleas et al, who used the standard HPLC method, 15 we found similar amounts of trans-resveratrol in grapes. For example, the trans-resveratrol content in merlot grapes was 6.77 mg/g fresh weight in our study; it was 7.039 and 5.64 mg/g according to Okuda et 34 Based on our results and others, obviously, different varieties of grapes have different contents of resveratrol, and the same case exists even in the same variety from one vintage to another as well as the developmental stages of grapes.…”

“…High-performance liquid chromatography (HPLC) with UV absorbance detection, fluorometric detection and diode-array detector is the most common method. [16][17][18] However, this method normally takes a long time, which is still not ideal for resveratrol 1020 ANALYTICAL SCIENCES AUGUST 2008, VOL. 24 analysis, considering the unstable property of this compound.…”

HPLC and HPTLC methods for Simultaneous Estimation of Quercetin and Curcumin in Polyherbal Formulation

“…Also a number of validated HPLC methods have been devised for the estimation of QCN both individually and in combination with other compounds [24][25][26][27] . Quantification of CMN has been done by HPTLC technique [28] .…”

Optimization of Quercetin Extraction from Green Tea ( Camellia sinensis ) Using Central Composite Design, and the Pharmacological Activity of the Extract

“…For identification of the extracted quercetin, preparative TLC 12 , RP-HPLC 11,13,14,[20][21][22] , micellar liquid chromatography 23 and GC-MS 24 were used. A traditional approach, well known as the "One Variable At a Time" (OVAT), is commonly applied for investigation of the effects of extraction param- eters on the yield of the desired compounds.…”