Analysis of trans-Resveratrol in Grapes by Micro-High Performance Liquid Chromatography

Abstract

Resveratrol, existing in two isomeric trans-and cis-forms, is a phytoalexin that has pharmacological activities, like anticancer and anti-cardiovascular diseases as well as life-span increasing properties. Grapes are good sources for resveratrol introduction in the human diet not only directly, but also through its products: wine. In the present work, trans-resveratrol determination and the separation of resveratrol isomers were investigated by the use of micro-high performance liquid chromatography. The analysis conditions were optimized. The baseline separation of trans-and cis-resveratrol was obtained. Further, a trans-resveratrol determination of some grapes growing in Shacheng, Huailai, Hebei province, China was carried out with our analytical method. The concentrations were 6.77 ± 0.12 and 3.95 ± 0.18 (mg/g fresh weight) for merlot and cabernet sauvignon grapes, respectively.

Water stress and abscisic acid exogenous supply produce differential enhancements in the concentration of selected phenolic compounds in Cabernet Sauvignon

“…Furthermore, since other compounds present in the matrix may contribute to the absorbance, these methods are characterized by poor selectivity. To date, a number of analytical methods are available for the simultaneous determination of grape and wine phenolics including those based on separation techniques such as Gas and Liquid Chromatography (GC, HPLC) and Capillary Electrophoresis (CE) [27][28][29][30][31][32]. Nevertheless, even the latest chromatography techniques still produce relatively limited results and in many cases structural analysis still remains unclear.…”

Current separative strategies used for resveratrol determination from natural sources

“…The recovery of this procedure only reaches 85.1%, indicating further investigations are required because other factors such as temperature, pH, storage of the sample and oxidation all potentially affect the extraction efficiency and have to be optimized. 11 At this point, it is interesting to mention that according to Karacabey et al, who studied the effect of temperature and ethanol concentration on the extraction of trans-resveratrol, trans-3-viniferin, ferulic acid, and total phenolics from milled grape canes, a higher operation temperature might improve the resolvation of resveratrol in a relatively short time, since the effective diffusivity and the solubility of the extracted compounds increased with increasing temperature. 27 Moreover, different solvents showed variable extraction efficiencies and it turned out that 0.1% HCl acidified methanol and MeOH had a higher extraction efficiency, while the model solution of wine (12% EtOH) and ethyl acetate (EtOAc) extracted only small amounts of trans-resveratrol.…”

“…has recently attracted great interest for resveratrol analysis. 11,46 m-HPLC has the advantages of lowering the detection limits and increasing the method sensitivity because a smaller diameter column improves the sensitivity. A micro-column could also lower solvent consumption because of its low flow rate and improve high temperature compatibility owing to its low thermal mass, thereby improving column efficiency since higher column temperatures increase the mass transfer rate.…”

“…From natural sources, resveratrol has been found in more than 72 plant species, including grapes, Japanese knotweed, peanuts, eucalyptus, spruce, lily, mulberries, and groundnuts. 9 Among them, grapes and their products, wine, are believed to be able to provide an abundant and direct source of this compound for human beings, 10,11 which also constitutes the basis of the famous ''French paradox'' phenomenon, in which a low incidence of cardiovascular disease is reported despite a high-fat diet in France. 12 The concentration of resveratrol varies in different wines, ranging from 660 to less than 0.68 mg mL À1 , and from 100 to less than 0.23 mg mL À1 , in red and white wines, respectively.…”

Ultrasound-assisted extraction of resveratrol from grape leaves and its purification on mesoporous carbon

“…Grape is an everyday plant in China and widely known for its edible fruit. There have been numerous reports on the extraction of polyphenolic antioxidants from grape fruit, seeds, and skin (Beres et al, 2017;Fan et al, 2008;Li et al, 2011;Pascual-Martí et al, 2001;Sant'Anna et al, 2012). While these parts of the grape have been intensively examined, there have been few reports on the use of grape leaves, which are usually discarded.…”