HPLC and HPTLC methods for Simultaneous Estimation of Quercetin and Curcumin in Polyherbal Formulation

Abstract

Baghel, et al.: Chromatographic Analysis of Quercetin and CurcuminFast, sensitive, precise and selective high performance liquid chromatography and high performance thin layer chromatography methods were developed for the simultaneous estimation of curcumin and quercetin in a polyherbal formulation. Validation of these methods were carried out with respect to linearity, range, precision, accuracy and robustness. Both methods were applied to a polyherbal formulation to find the amount of curcumin and quercetin and compared. Curcumin and quercetin were found to be 9.5 mg and 0.613 mg in the polyherbal formulation using the high-performance liquid chromatography method whereas with the high performance thin layer chromatography method, curcumin and quercetin were found to be 9.7 mg and 0.674 mg, respectively. Both methods identified and quantified curcumin and quercetin effectively.

Unusual Salt and pH Induced Changes in Polyethylenimine Solutions

“…The assay was performed on 4.08 mM PEI solutions in 150 mM NaCl at various pH. Secondary amines react with ninhydrin in acidic medium to give iminium salt [ 30 ]. The iminium salt has a characteristic yellow color with optimum UV-Vis absorbance at 440 nm.…”

“…The formation of the iminium salt requires both a transferable electron pair on the amine nitrogen and an acidic medium. The reaction between ninhydrin and a secondary amine to form the iminium salt proceeds in two stages: (1) the lone-pair of electrons from the nitrogen of PEI’s secondary amine is transferred to the ninhydrin complex; and (2) the ninhydrin complex undergoes hydrolysis in the acidic medium to form iminum salt [ 30 ]. Acidic pH decreases iminium salt formation in linear PEI (i.e., absorbance decreases in the ‘weak acidic’ region, Fig 6D ), which can be attributed to the decrease in the number of nitrogen atoms being able to donate lone-pair of electrons as they become protonated.…”

DNA protection activity of a hydroethanol extract and six polyphenol monomers fromMorus alba L. (mulberry) twig

“…Recent discoveries in the field of Morus alba L. phytochemicals revealed that Morus alba L. twig extracts contain polyphenols, which are major effective antioxidants. [26][27][28][29] Meanwhile, monomer polyphenols including oxyresveratrol, resveratrol [30,31] , mulberroside, rutin [32] , quercetin [33] , and morin, [34] which exist in Morus alba L. twig are more extensive, have high antioxidant activity, and are effective scavengers of free radicals. Six polyphenols, in particular, including rutin, quercetin, and morin, have been isolated and shown to possess high free radical scavenging activity [35] , and resveratrol and rutin can protect against DNA damage.…”

Chemometric-Assisted UV-Spectrophotometric Method for Quantification of Novel Anti-Tubercular Liquisolid Formulation

“…According to the literature, numerous methods, including UV spectroscopy [16,17], RP-HPLC [18][19][20], HPTLC [21,22] and others, are available for determining RIF both alone and in conjunction with various drugs in a variety of dose forms. A few methods, including HPLC [23][24][25][26], UV spectroscopy [27] and HPTLC [28][29][30][31], have been taken into consideration for the assessment of QUE both alone and in conjunction with different drugs in different forms of dosage. The previously mentioned chromatographic strategies are broadly utilized and suggested; however, these techniques require mind-boggling and costly instrumentation, arrangement for use and removal of solvents, work escalated test planning methodology and individual aptitudes [32,33].…”