Direct grafting of RGD-motif-containing peptide on the surface of polycaprolactone films

Abstract

Direct surface modification of biodegradable polycaprolactone (PCL) was performed without the necessity of synthesis of functionisable co-polymers. An easy-to-perform three-step procedure consisting of amination, reaction with hetero-bifunctional cross-linkers and conjugation of an RGD-motif-containing peptide was used to modify polymer films and improve the attachment of endothelial cells. The biological activity of modified surfaces was assessed by estimating microvascular endothelial cell attachment. Covalent coating with RGD resulted in an approximately 11-fold increase of endothelial cell attachment on modified PCL surfaces compared with untreated polymer. The specificity of the attachment enhancement was confirmed by using a control peptide. It is concluded that chemical surface modification is an appropriate method of rendering degradable polymers, such as PCL, cell-adhesive.

Molecular Understanding of Endothelial Cell and Blood Interactions with Bacterial Cellulose: Novel Opportunities for Artificial Blood Vessels

“…Additionally, protein adsorption to BC is very low. Much attention has focused on cell adhesion peptide RGD and its derivatives as possible alternative for stimulating reproducible and predictable cell adhesion (D'Souza et al, 1991;Hersel et al, 2003;Walluscheck et al, 1996;Gabriel et al, 2006). Most RGD modifications occur via covalent binding to the material (Massia & Hubbell, 1990).…”

Functionalization of poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) films via surface‐initiated atom transfer radical polymerization: Comparison with the conventional free‐radical grafting procedure

“…The amine quantity obtained (1.9 Â 10 À7 mol/cm 2 ) was in good agreement with results obtained by other authors for the aminolysis of PET 30 and PCL. 31, 32 Because of the hydrophobic nature and high crystallinity of PHBHV, we supposed that the aminolysis cleavage was confined to the surface region of the PHBHV, which ensured that the resulting grafting polymer was restricted to the surface.…”

Radiolabeled gelatin type B analogues can be used for non-invasive visualisation and quantification of protein coatings on 3D porous implants

“…The good solubility of PCL in several organic solvents, its low melting point (59-64°C), low economic cost and exceptional blend-compatibility has stimulated extensive research into its potential application in the biomedical field [3][4][5][6][7][8][9][10][11][12][13][14]. However, a drawback is its hydrophobic nature, the poor cell attachment and poor proliferation rate [15][16][17][18]. Consequently, several surface modification strategies have been investigated in order to enhance cell adhesion and improve the biocompatibility of PCL [15][16][17][18][19][20][21][22][23][24][25][26][27][28][29].…”

“…However, a drawback is its hydrophobic nature, the poor cell attachment and poor proliferation rate [15][16][17][18]. Consequently, several surface modification strategies have been investigated in order to enhance cell adhesion and improve the biocompatibility of PCL [15][16][17][18][19][20][21][22][23][24][25][26][27][28][29]. Most of these surface-modification strategies involve the immobilization of a biomolecule on the surface of the implant [27], which is mostly studied on 2D-polymer films.…”