Direct gas chromatographic analysis of sulphur compounds in mouth air in man

Tonzetich, J.

doi:10.1016/0003-9969(71)90062-8

Cited by 317 publications

(221 citation statements)

References 13 publications

Supporting

Mentioning

208

Contrasting

Unclassified

Order By: Relevance

“…The odour may reduce with debridement with dentifrice or when the individual breaks their fast. 2 A patient presenting at the dental practice during a fast may show signs of irritability or a degree of cognitive impairment. This has been shown to be associated with the lack of intake of addictive substances such as nicotine and caffeine.…”

Section: Fastingmentioning

confidence: 99%

The management of the Muslim dental patient

Darwish

2005

Br Dent J

View full text Add to dashboard Cite

Section: Fastingmentioning

confidence: 99%

The management of the Muslim dental patient

Darwish

2005

Br Dent J

View full text Add to dashboard Cite

“…Hydrogen sulfide (H 2 S) is a gas with the smell of rotten eggs and is one of the predominant volatile sulfur compounds primarily responsible for halitosis (Tonzetich, 1971). Oral malodour appears to be associated with an increase in the number of H 2 S-producing micro-organisms among tongue biofilm microflora (Washio et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Production of hydrogen sulfide by two enzymes associated with biosynthesis of homocysteine and lanthionine in Fusobacterium nucleatum subsp. nucleatum ATCC 25586

et al. 2010

View full text Add to dashboard Cite

Fusobacterium nucleatum produces a large amount of the toxic metabolite hydrogen sulfide in the oral cavity. Here, we report the molecular basis of F. nucleatum H2S production, which is associated with two different enzymes: the previously reported Cdl (Fn1220) and the newly identified Lcd (Fn0625). SDS-PAGE analysis with activity staining revealed that crude enzyme extracts from F. nucleatum ATCC 25586 contained three major H2S-producing proteins. Two of the proteins with low molecular masses migrated similarly to purified Fn0625 and Fn1220. Their kinetic values suggested that Fn0625 had a lower enzymic capacity to produce H2S from l-cysteine (∼30 %) than Fn1220. The Fn0625 protein degraded a variety of substrates containing βC–S linkages to produce ammonia, pyruvate and sulfur-containing products. Unlike Fn0625, Fn1220 produced neither pyruvate nor ammonia from l-cysteine. Reversed-phase HPLC separation and mass spectrometry showed that incubation of l-cysteine with Fn1220 produced H2S and an uncommon amino acid, lanthionine, which is a natural constituent of the peptidoglycans of F. nucleatum ATCC 25586. In contrast, most of the sulfur-containing substrates tested, except l-cysteine, were not used by Fn1220. Real-time PCR analysis demonstrated that the fn1220 gene showed several-fold higher expression than fn0625 and housekeeping genes in exponential-phase cultures of F. nucleatum. Thus, we conclude that Fn0625 and Fn1220 produce H2S in distinct manners: Fn0625 carries out β-elimination of l-cysteine to produce H2S, pyruvate and ammonia, whereas Fn1220 catalyses the β-replacement of l-cysteine to produce H2S and lanthionine, the latter of which may be used for peptidoglycan formation in F. nucleatum.

show abstract

“…VSCs, composed of hydrogen sulfide, methyl mercaptan, and dimethyl sulfide, have been known as the main factor of halitosis. 11,12 It can be measured either by halimeter easily or gas chromatography from aspirated breath airs. [13][14][15] Among these VSCs, endogenous hydrogen sulfide (H2S) is synthesized from most tissues in mammals, 16,17 for which two enzymes are involved in this synthesis, cystathionine β-synthase (CBS) and cystathionine γ-lyase (CSE).…”

Section: Introductionmentioning

confidence: 99%

Volatile Sulfur Compounds as a Predictor for Esophagogastroduodenal Mucosal Injury

Yoo

Jung²,

Sohn³

et al. 2008

Gut Liver

View full text Add to dashboard Cite

Background/Aims: Halitosis is a symptom that bothers patients more socially than medically and its pathogenic mechanisms are unclear and treatment armamenterium is limited. Clinicians generally ignored active interventions. Since halitosis is closely associated with volatile sulfur compounds (VSCs), we used a Halimeter and gas chromatography to measure VSCs in patients with Helicobacter-pylori (H. pylori)-associated gastric diseases. Methods: We categorized 72 patients with H. pylori infection into two groups based on their endoscopic findings: a nonerosive mucosal group (NE, n=24) and an erosive mucosal group (E, n=48). Halitosis was objectively assessed by applying either a Halimeter to breath air or gas chromatography to gastric juice. Simultaneously, the expression of VSC-generating enzyme was measured with reverse-transcriptase PCR using mRNA isolated from biopsy tissues. Results: The levels of VSCs in exhaled breaths or aspirated gastric juices differed significantly between the NE and E groups (p ＜0.00001), suggesting that VSCs might reflect eroded epithelial damage induced by H. pylori infection. The expressions of cystathionine β-synthase (CBS) and cystathionine γ-lyase (CSE) were broadly consistent with the degree of mucosal injury. Conclusions: Erosive changes in esophagogastroduodenal mucosa were strongly correlated with increased VSC levels, suggesting that halitosis might result from H. pylori-associated erosive lesions. (Gut and Liver 2008;2: 113-118)

show abstract

Direct gas chromatographic analysis of sulphur compounds in mouth air in man

Cited by 317 publications

References 13 publications

The management of the Muslim dental patient

The management of the Muslim dental patient

Production of hydrogen sulfide by two enzymes associated with biosynthesis of homocysteine and lanthionine in Fusobacterium nucleatum subsp. nucleatum ATCC 25586

Volatile Sulfur Compounds as a Predictor for Esophagogastroduodenal Mucosal Injury

Contact Info

Product

Resources

About