Direct determination of indole‐3‐acetic acid in plant tissues by electroanalytical techniques using a carbon paste modified with OV‐17 electrode

Hernández, Pedro; Galán, Fernando; Nieto, Óscar; Hernández, L.

doi:10.1002/elan.1140060708

Cited by 33 publications

(9 citation statements)

References 9 publications

(1 reference statement)

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“…On the other hand some electroanalytical methods have been reported for quantitative determination of IAA using a carbon paste electrode modified with a silicone OV-17 [16], a carbon fibre ultra microelectrode [17], glassy carbon electrode coated with carbon nanotubes film [18], carbon paste electrode modified with sodium dodecylsulfate [19], and graphite-polyurethane composite electrode [20] in plant extracts or in soil samples.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Behavior and Electroanalytical Determination of Indole‐3‐Acetic Acid Phytohormone on a Boron‐Doped Diamond Electrode

Yardım

Erez

2010

Electroanalysis

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In this work, a boron-doped diamond (BDD) electrode was used for the electroanalytical determination of indole-3-acetic acid (IAA) phytohormone by square-wave voltammetry. IAA yielded a well-defined voltammetric response at + 0.93 V (vs. Ag/AgCl) in Britton-Robinson buffer, pH 2.0. The process could be used to determine IAA in the concentration range of 5.0 to 50.0 mM (n = 8, r = 0.997), with a detection limit of 1.22 mM. The relative standard deviation of ten measurements was 2.09 % for 20.0 mM IAA. As an example, the practical applicability of BDD electrode was tested with the measurement of IAA in some plant seeds.

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Section: Introductionmentioning

confidence: 99%

Electrochemical Behavior and Electroanalytical Determination of Indole‐3‐Acetic Acid Phytohormone on a Boron‐Doped Diamond Electrode

Yardım

Erez

2010

Electroanalysis

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“…For measurement of IAA near plant tissue surfaces, in situ electrochemical microsensors overcome many of these problems. However, when used in a traditional concentration mode that measures the amount of a molecule of interest, microsensors suffer from low signal-to-noise ratio, temporal drift, and relatively high detection limits (Hernandez et al, 1994). In contrast, self-referencing (SR) microsensors (Porterfield, 2007) have the potential to overcome these limitations.…”

Section: Introductionmentioning

confidence: 99%

Non-invasive quantification of endogenous root auxin transport using an integrated flux microsensor technique

et al. 2010

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SUMMARYIndole-3-acetic acid (IAA) is a primary phytohormone that regulates multiple aspects of plant development. Because polar transport of IAA is an essential determinant of organogenesis and dynamic tropic growth, methods to monitor IAA movement in vivo are in demand. A self-referencing electrochemical microsensor was optimized to non-invasively measure endogenous IAA flux near the surface of Zea mays roots without the addition of exogenous IAA. Enhanced sensor surface modification, decoupling of acquired signals, and integrated flux analyses were combined to provide direct, real time quantification of endogenous IAA movement in B73 maize inbred and brachytic2 (br2) auxin transport mutant roots. BR2 is localized in epidermal and hypodermal tissues at the root apex. br2 roots exhibit reduced shootward IAA transport at the root apex in radiotracer experiments and reduced gravitropic growth. IAA flux data indicates that maximal transport occurs in the distal elongation zone of maize roots, and net transport in/out of br2 roots was decreased compared to B73. Integration of short term real time flux data in this zone revealed oscillatory patterns, with B73 exhibiting shorter oscillatory periods and greater amplitude than br2. IAA efflux and influx were inhibited using 1-N-naphthylphthalamic acid (NPA), and 2-naphthoxyacetic acid (NOA), respectively. A simple harmonic oscillation model of these data produced a correlation between modeled and measured values of 0.70 for B73 and 0.69 for br2. These results indicate that this technique is useful for real-time IAA transport monitoring in surface tissues and that this approach can be performed simultaneously with current live imaging techniques.

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“…Hernández et al. 11 determined 51.2 µg of IAA in 1 g of Ecballium elaterium L. plant using differential pulse voltammetry with a carbon paste electrode modified with silicone OV‐17. Square wave voltammetry using a graphite‐polyurethane composite electrode 12 reached low limits of quantification ( LOQ 1.3 ⋅ 10 −6 mol L −1 ).…”

Section: Introductionmentioning

confidence: 99%

Determination of Plant Hormone Indole‐3‐Acetic Acid in Aqueous Solution

et al. 2012

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An electroanalytical method was developed for the determination of the phytohormone indole-3-acetic acid in water using a glassy-carbon electrode. The differential pulse voltammetry was combined with a purification step which involved the extraction of plant tissue and the separation of plant pigments by passing through column Oasis MCX. Indole-3-acetic acid can be determined under optimum conditions with a limit of quantification (LOQ) 2.7 106 mol L1. The linearity range was from 1.8106 to 6.6104 mol L1. The electroanalytical determination of indole-3-acetic acid is selective, fast and economic. The presence of other phytohormones as abscisic acid and cytokinins as isopentenyladenine, isopentenyladenosine, trans-zeatin, zeatin riboside does not interfere the determination of indole-3-acetic acid in plant sample

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Direct determination of indole‐3‐acetic acid in plant tissues by electroanalytical techniques using a carbon paste modified with OV‐17 electrode

Cited by 33 publications

References 9 publications

Electrochemical Behavior and Electroanalytical Determination of Indole‐3‐Acetic Acid Phytohormone on a Boron‐Doped Diamond Electrode

Electrochemical Behavior and Electroanalytical Determination of Indole‐3‐Acetic Acid Phytohormone on a Boron‐Doped Diamond Electrode

Non-invasive quantification of endogenous root auxin transport using an integrated flux microsensor technique

Determination of Plant Hormone Indole‐3‐Acetic Acid in Aqueous Solution

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