Determination of Plant Hormone Indole‐3‐Acetic Acid in Aqueous Solution

Abstract

An electroanalytical method was developed for the determination of the phytohormone indole-3-acetic acid in water using a glassy-carbon electrode. The differential pulse voltammetry was combined with a purification step which involved the extraction of plant tissue and the separation of plant pigments by passing through column Oasis MCX. Indole-3-acetic acid can be determined under optimum conditions with a limit of quantification (LOQ) 2.7 106 mol L1. The linearity range was from 1.8106 to 6.6104 mol L1. The electroanalytical determination of indole-3-acetic acid is selective, fast and economic. The presence of other phytohormones as abscisic acid and cytokinins as isopentenyladenine, isopentenyladenosine, trans-zeatin, zeatin riboside does not interfere the determination of indole-3-acetic acid in plant sample

Real‐Time Monitoring of Auxin Vesicular Exocytotic Efflux from Single Plant Protoplasts by Amperometry at Microelectrodes Decorated with Nanowires

“…Note that this value is much lower than previously reported (usually over the range of 0.70–0.90 V13b–d, 16) and also lower than that on TiC@C‐QANFAs (Supporting Information, Figure S2), indicating the excellent electrocatalytic properties toward oxidation of IAA. The calculated electron stoichiometry was two, affording a cation of 3‐methyleneindolenine carboxylic acid, in agreement with previous reports 13a–d. The amperometric response of 0.7 μ M IAA at TiC@C/Pt‐QANFAs microsensor is approximately 5‐fold greater than that at TiC@C‐QANFAs (Figure 2 d).…”

“…Figure 2 e shows the amperometric responses of the TiC@C/Pt‐QANFAs microsensor to successive additions of IAA. Furthermore, this sensor displays excellent linear amperometric response over the IAA concentration range of interest (Figure 2 f), with a detection limit of 1.0 n M (S/N=3), being at least 20 times better than those reported previously 13a–d…”

A Novel Electrochemical Sensor Based on Modified Carbon Paste Electrode with ZnO Nanorods for the Voltammetric Determination of Indole‐3‐acetic Acid in Plant Seed Extracts

“…Alternatively, electrochemical methods with the advantages of high sensitivity, low cost, rapid response, and simple apparatus make them suitable alternatives of detection for such compounds. Many electrochemical sensors for the determination of IAA have been used [11][12][13][14][15][16]. Carbon paste electrodes (CPEs) have been applied in various electrochemistry applications because of their advantages over mercuryfree solid electrodes in terms of ease of preparation, speed, stable response, porous surface, renewability, low cost, low ohmic resistance, and no need for the internal solution [17][18][19][20][21][22].…”

Interference-free Determination of Indole-3-Acetic Acid in Two Real Systems Using Second-order Calibration Method Coupled with Excitation-emission Matrix Fluorescence

“…Several analytical methods have been developed for the determination of IAA in these matrices, such as differential pulse voltammetry (DPV), 7 high performance liquid chromatography (HPLC), 8 liquid chromatography-electrospray tandem mass spectrometry (LC-ESI-MS/MS), 9,10 and hollow fiber-based liquid-liquid-liquid microextraction combined with high performance liquid chromatography-ultraviolet detection (HF-LLLME/HPLC-UV). 11 However, these methods are usually accompanied by time-consuming and material-consuming procedures and also are a cause of environmental pollution.…”